Study on utilization technology of oxygen-tolerant denitrifying microorganisms in sewage and wastewater treatment to prevent global warming

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In this research project, we developed an effective method to monitor the behavior of nitrous oxide reductase gene (nosZ) in nitrification and denitrification processes by using quantitative PCR (realtime PCR) method. After the development, Pseudomonas stutzeri TR2 that was known as an oxygen tolerant bacterium was introduced into nitrification and denitrification systems, and its survival and denitrification activity were investigated. New denitrifying bacteria were isolated and selected under aerobic and nitrite-existing conditions from activated sludge obtained from a wastewater treatment plant. Among the 20 bacterial isolates, some of them were exhibited high denitrification capability with no emission of nitrous oxide under existing dissolved oxygen. These isolates also show high growth capability with nitrous oxide as a sole source of electron acceptor. Therefore, these isolates originated from activated sludge were considered to be applicable to the technology for reduction of nitrous oxide emission.