Phylogenetic Evolution of Vertebrate Mitochondrial DNA

脊椎动物线粒体DNA的系统发育进化

• 批准号: 09640739
• 负责人: HASEGAWA Masami
• 金额: $ 1.86万
• 依托单位: Institute of Statistical Mathematics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09640739/
• 关键词: mitochondrial DNA; protein; molecular phylogeny; ND1; vertebrate; eutheria; lamprey; shark; NDI; ヤツメウナギ; ND1遺伝子; 肺魚; シ-ラカンス; 収斂進化

Several cases of misleading molecular phylogenies estimated from mitochondrial DNA-encoded proteins have been known. In order to clarify the causes of these misleading phylogenies, we conducted several new analyses.1) ND1 gene gives contradictory tree from that estimated from other genes of mitochondria concerning phylogeny among eutherian orders. In order to distinguish stochastic error due to to small species sampling from convergent substitutions due to some adaptive evolution, we sequenced ND1 gene from several eutherian species as well as several outgroup marsupials.It turned out that convergent evolution has occurred in ND1 between primates and rodents or between ferungulates and outgroup.2) By using lamprey as an outgroup in the analysis of mitochondrial proteins, a bizarre tree is obtained in which lamprey groups with lungfish in the vertebrate tree. In order to clarify the reason of this, we sequenced complete mtDNA from shak. With this data as an outgroup, morphologically acceptable tree was obtained, demonstrating that something strange occurred in the lamprey and/or lungflsh lineages.

已经知道几个从线粒体DNA编码的蛋白质估计的误导性分子遗传学的案例。为了阐明这些错误的遗传学解释的原因，我们进行了几个新的分析：1）ND 1基因与线粒体其他基因关于真兽目遗传学的估计结果相矛盾。为了区分小种群抽样引起的随机误差和适应性进化引起的趋同替换，我们对几种真兽目动物和几种外群有袋类动物的ND 1基因进行了测序，结果表明，ND 1基因在灵长类和啮齿类动物之间以及有蹄类动物和外群动物之间发生了趋同进化。得到一个奇异树，其中七鳃鳗组与肺鱼在脊椎动物树中。为了阐明这一现象的原因，我们对该菌株的线粒体DNA进行了全序列测定。以这些数据作为外群，获得了形态学上可接受的树，表明七鳃鳗和/或肺鱼谱系中发生了一些奇怪的事情。

项目成果

Cao,Y., Hasegawa,M.: "Phylogenetic position of guineapigs reuisited" Malecular Biology and Evalution. 14. 461-464 (1997)

曹，Y.，长谷川，M.：“豚鼠的系统发育位置重新定位”男性生物学和评估。

T.Hashimoto, L.Sanchez, T.Shirakura, M.Muller, and M.Hasegawa: "Secondary absence of mitochondria in Giardia lamblia and Trichomonas vaginalis revealed by valyl-tRNA synthetase phylogeny." Proc.Natl.Acad.Sci.USA.95. 6860-6865 (1998)

T.Hashimoto、L.Sanchez、T.Shirakura、M.Muller 和 M.Hasekawa：“缬氨酰-tRNA 合成酶系统发育揭示了贾第鞭毛虫和阴道毛滴虫中线粒体的继发性缺失。”

M.Hasegawa,Y.Cao: "Preponderance of slightly deleterious polymorphism in mitchondrial DNA:Non-synonymous/synonymous rate ratio is higher within species than between species." Mol.Biol.Evol.15. 1499-1505 (1998)

M.Hasekawa，Y.Cao：“线粒体DNA中轻微有害多态性的优势：物种内的非同义/同义比率比物种间更高。”

Y.Cao, M.Hasegawa: "Conflict among individual mitochondorial proteins in resolving the phylogeny of eatherian orders" J. Mol. Evol.47. 307-322 (1998)

Y.Cao，M.Hasekawa：“个体线粒体蛋白在解决亚兽目系统发育方面的冲突”J. Mol。

Y.Cao, P.J.Waddell, N.Okada, and M.Hasegawa: "The complete mitochondrial DNA sequence of the shark Mustelus manazo : Resolving vertebrate phylogeny with mitochondrial genome sequences when all known methods fail completely." Mol.Biol.Evol.15. 1637-1646 (1

Y.Cao、P.J.Waddell、N.Okada 和 M.Hasekawa：“鲨鱼 Mustelus manazo 的完整线粒体 DNA 序列：当所有已知方法完全失败时，用线粒体基因组序列解决脊椎动物系统发育问题。”