Steroid Hormone Receptor-Containing Neurons and Differentiation of Neuroendocrine Functions

类固醇激素受体神经元与神经内分泌功能的分化

• 批准号: 09640799
• 负责人: HAYASHI Shinji
• 金额: $ 1.98万
• 依托单位: TOKYO METROPOLITAN INSTITUTE FOR NEUROSCIENCE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09640799/
• 关键词: Estrogen Receptor; Androgen Receptor; Aromatase; Immunohistochemistry; RT-PCR; Gene expression; Hypothalamus; Hippocampus; エストロゲン受容体

Role of neurons that contain steroid hormone receptors, such as estrogen or androgen receptors in the development and differentiation of the brain was analyzed. By using immunohistochemistry (IHC), in situ hybridization histochemistry (ISHH) and reverse transcriptase-polymerase chain reaction (RT-PCR), we have already disclosed that estrogen receptor alpha (ERalpha) is expressed in the preoptic region, the hypothalamus and the amygdala in the rat brain. Moreover, we have disclosed ERalpha is also detected in the hylar region of the hippocampus both by IHC and ISHH.They were located sporadically in the polymorphic neurons of the hylus and granular cells of the cornus. No distinct sex difference was detected. In addition, androgen receptor immunoreactivity was detected selectively in the ventral premammillary nucleus that is located in the posterior region of the hypothalamus. The androgen receptor immunoreactivity in this nucleus increased by administration of androgen. Since no aromatase that converts testosterone into estradiol was detected in this nucleus, direct action of androgen on the neurons of this nucleus was suggested. On the other hand, a new kind of estrogen receptor that was found recently in the rat prostate and named as ERbeta is also detected in the brain. We also confirmed the expression of ERbeta in the rat brain by IHC and ISHH.

分析了含有类固醇激素受体（如雌激素或雄激素受体）的神经元在脑发育和分化中的作用。通过免疫组织化学（IHC）、原位杂交组织化学（ISHH）和逆转录-聚合酶链反应（RT-PCR），我们已经发现雌激素受体α（ER α）在大鼠脑内的视前区、下丘脑和杏仁核中均有表达。此外，我们还发现ER α在海马的hylar区也有表达，它们散在分布于海马hylar区的多态性神经元和海马cornus的颗粒细胞中。未发现明显的性别差异。此外，雄激素受体免疫反应性检测选择性腹前乳头体核，位于下丘脑后部区域。在这个核的雄激素受体的免疫反应性增加的管理雄激素。由于没有芳香化酶，转换睾酮为雌二醇检测在这个核，雄激素的直接作用于这个核的神经元建议。另一方面，最近在大鼠前列腺中发现的一种新的雌激素受体，命名为ER β，也在大脑中检测到。我们还通过IHC和ISHH证实了大鼠脑中ER β的表达。

项目成果

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Hayashi, S. 等人：“生殖的神经控制：生理学和行为”Maeda, K. 和 Tsukamura, H.（编辑）日本协会出版中心，东京，135-152（1997）

Yang,Y.,et al.: "Immunocytochenmical analysis of sex differences in calcitonin gene-related peptide in the rat dorsal root ganglion,with special reference to estrogen and its receptor." Brain Res. 791. 35-42 (1998)

Yang，Y.，et al.：“大鼠背根神经节降钙素基因相关肽性别差异的免疫细胞化学分析，特别是雌激素及其受体。”

Yang, Y., Ozawa, H., Lu, H., Yuri, K., Hayashi, S., Nihonyanagi, K.and Kawata, M.: "Immunocytochemical analysis of sex differences in calcitonin gene-related peptide in the rat dorsal root ganglion, with special reference to estroger and its receptor." Br

Yang, Y.、Ozawa, H.、Lu, H.、Yuri, K.、Hayashi, S.、Nihonyanagi, K. 和 Kawata, M.：“大鼠降钙素基因相关肽性别差异的免疫细胞化学分析

Orikasa, C.and Hayashi, S.: "Studies and problems of estrogen receptors in the regulation of the neuroendocrine system (in Japanese)" Human Science. 10. 48-56 (1998)

Orikasa, C. 和 Hayashi, S.：“雌激素受体在神经内分泌系统调节中的研究和问题（日语）”人类科学。

Yang,Y.,et.al.: "Immunocytochemical analysis of sex differences in calcitonin gene-related peptide in the rat dorsal root ganglion, with special reference to estrogen and its receptor." Brain Res. 791. 35-42 (1998)

Yang,Y.,et.al.：“对大鼠背根神经节降钙素基因相关肽的性别差异进行免疫细胞化学分析，特别是雌激素及其受体。”