Interface Ecology: How do macroalgae control the quantity and composition of bacterial epibioses at their surfaces?

界面生态学：大型藻类如何控制其表面细菌表生的数量和组成？

• 批准号: 86566400
• 负责人: Professor Dr. Martin Wahl, Ph.D.
• 金额: --
• 依托单位: GEOMAR Helmholtz-Zentrum für Ozeanforschung Kiel
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/86566400?language=en
• 关键词: Interface; Ecology; How; do; macroalgae

Epiphytic bacterial communities have the proven potential to profoundly modify the functional interface between macroalgae and their environment. Pilot studies strongly indicate that biofilms on cooccurring macroalgal species differ fundamentally even when they originate from the same colonizer pool. However, the specific composition of biofilms on different hosts or the algal capacity to control its biofilm is still largely unknown. The proposed research will fill this gap for three different macroalgae of great ecological and economical importance: Fucus vesiculosus, Gracilaria vermiculophylla, Ulva compressa. We aim to analyse specificity, structure and variability of biofilms on these macroalgae and the host’s chemical control over these biofilm characteristics. The epibacterial communities will be characterized by 16S rDNA analysis (degenerating gel gradient electrophoresis and sequencing) followed by sequencing. Using a novel technique which permits to simulate the continuous excretion process on algal surfaces, natural surface extracts of macroalgae will be tested for their capacity to control the formation, composition and dynamics of natural marine biofilms.

附生细菌群落已被证明具有深刻改变大型藻类与其环境之间功能界面的潜力。初步研究强烈表明，共存的大型藻类物种上的生物膜存在根本差异，即使它们源自同一定殖池。然而，不同宿主上生物膜的具体组成或藻类控制其生物膜的能力仍然很大程度上未知。拟议的研究将填补三种具有重大生态和经济重要性的不同大型藻类的空白：墨角藻、江蓠、石莼。我们的目标是分析这些大型藻类生物膜的特异性、结构和变异性以及宿主对这些生物膜特征的化学控制。表细菌群落将通过 16S rDNA 分析（简并凝胶梯度电泳和测序）进行表征，然后进行测序。使用允许模拟藻类表面连续排泄过程的新技术，将测试大型藻类的天然表面提取物控制天然海洋生物膜的形成、组成和动态的能力。