Monitoring method of human cytomegalovirus latent infection and viral reactivation

人巨细胞病毒潜伏感染和病毒再激活的监测方法

• 批准号: 09557028
• 负责人: KONDO Kazuhiro
• 金额: $ 7.49万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557028/
• 关键词: cytomegalovirus; latency; reactivation; NASBA; 潜伏感染; 骨髄移植; 臓器移植; mRNA; AIDS

Human cytomegalovirus (HCMV) is a significant cause of morbidity and mortality in immunocompromised patients, such as organ or bone marrow transplant (BMT), AIDS patients and developing neonates. Some antiviral drugs, such as ganciclovir and foscarnet, are useful for treating HCMV diseases, but these drugs must be used carefully because of their side effects. Therfore, it is important to establish a sensitive system by which to monitor HCMV infection.In the diagnosis of HCMV infection, it is very important to distinguish symptomatic from asymptomatic infection. The nucleic acid sequence-based amplification (NASBA) technique was compared with single and nested polymerase chain reaction (PCR) methods.For NASBA detection, the beta 2.7 transcript was chosen as a target because of its abundant active HCMV-specific expression. Of 20 pediatric BMT recipients, 8 developed HCMV-related clinical symptoms. The clinical sensitivities and specificities were 50% and 100% for single PCR, 100% and 67% for nested PCR, and 100% and 83% for NASBA, respectively. Follow-up of HCMV infections in pediatric BMT recipients showed that NASBA could both detect viral transcript prior to the onset of clinical symptoms and reflect clinical improvement due to antiviral therapy. These data suggest that NASBA should be useful for both predicting HCMV disease development and monitoring the effect of antiviral therapy.

人类巨细胞病毒 (HCMV) 是导致器官或骨髓移植 (BMT) 等免疫功能低下患者、艾滋病患者和发育中新生儿发病和死亡的重要原因。一些抗病毒药物，例如更昔洛韦和膦甲酸，可用于治疗 HCMV 疾病，但由于其副作用，必须谨慎使用这些药物。因此，建立一个敏感的HCMV感染监测系统非常重要。在HCMV感染的诊断中，区分有症状和无症状感染非常重要。将基于核酸序列的扩增 (NASBA) 技术与单一和巢式聚合酶链式反应 (PCR) 方法进行比较。对于 NASBA 检测，选择 β 2.7 转录物作为靶标，因为其丰富的活性 HCMV 特异性表达。在 20 名儿科 BMT 接受者中，8 名出现了 HCMV 相关的临床症状。单一 PCR 的临床敏感性和特异性分别为 50% 和 100%，巢式 PCR 的临床敏感性和特异性分别为 100% 和 67%，NASBA 的临床敏感性和特异性分别为 100% 和 83%。对儿科 BMT 接受者 HCMV 感染的随访表明，NASBA 既可以在临床症状出现之前检测到病毒转录本，又可以反映抗病毒治疗带来的临床改善。这些数据表明 NASBA 对于预测 HCMV 疾病发展和监测抗病毒治疗的效果应该有用。

项目成果

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