Analysis of human herpesvirus 6 latent infection in the brain.

人类疱疹病毒6型大脑潜伏感染分析。

• 批准号: 14570263
• 负责人: KONDO Kazuhiro
• 金额: $ 2.5万
• 依托单位: The Jikei University School of Medicine (2003)Osaka University (2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570263/
• 关键词: human herpesvirus 6; HHV-6; latency; latency-associated transcript; reactivation; immediate early gene; Heterogeneous Ribonucleoprotein K; Casein Kinase 2; Heterogeneous Ribonucleoprotein K; マクロファージ; 中枢神経系

Four kinds of latency-associated transcripts of human herpesvirus 6 were identified which were detected only in latently infected cells. Although they were oriented in the same direction as the immediate-early land 2 (IE1/IE2) genes and shared their protein-coding region with IE1/IE2, their transcription start sites and exon(s) were latency associated.Latency-associated transcripts of human herpesvirus 6 (H6LTs) (K.Kondo et al.J.Virol.76:4145-4151, 2002) were maximally expressed at a fairly stable intermediate stage between latency and reactivation both in vivo and in vitro. H6LTs functioned as sources of immediate-early protein 1 at this stage, which up-regulated the viral reactivation.The U3-U7 gene cluster of human herpesvirus 6 (HHV-6) was replaced with an enhanced green fluorescent protein-puromycin gene cassette containing the cytomegalovirus major immediate-early promoter. Neither viral replication in T cells nor latency and reactivation in macrophages was impaired. During HHV-6 latency, the cytomegalovirus promoter used the transcription start sites employed in cytomegalovirus latency.

鉴定了4种潜伏相关的人类疱疹病毒6型转录本，仅在潜伏感染的细胞中检测到。尽管它们与立即早期1和2（IE 1/IE 2）基因的方向相同，并且与IE 1/IE 2共享它们的蛋白质编码区，但它们的转录起始位点和外显子是潜伏相关的。人疱疹病毒6（H6 LT）的潜伏相关转录物（K.Kondo等，J.Virol.76：4145-4151，2002）在体内和体外潜伏和再活化之间的相当稳定的中间阶段最大表达。H6 LTs作为即刻早期蛋白1（immediately early protein 1，IMP 1）的来源，上调病毒的再活化，将人疱疹病毒6型（human herpesvirus 6，HHV-6）的U3-U 7基因簇替换为含有巨细胞病毒主要即刻早期启动子的增强型绿色荧光蛋白嘌呤霉素基因盒。病毒在T细胞中的复制和巨噬细胞中的潜伏期和再活化均未受损。在HHV-6潜伏期，巨细胞病毒启动子使用巨细胞病毒潜伏期中使用的转录起始位点。

项目成果

Kondo K et al.: "Identification of human herpesvirus 6 latency-associated transcripts"The Journal of Virology. 76・8. 4145-4151 (2002)

Kondo K 等人：“人类疱疹病毒 6 潜伏相关转录物的鉴定”病毒学杂志 76・8 4145-4151（2002）。

Nomura M, Kurita-Taniguchi M, Kondo K, et al.: "Mechanism of host cell protection from complement in murine cytomegalovirus (CMV) infection : identification of a CMV-responsive element in the CD46 promoter region"Eur J Immunol. 32・10. 2954-2964 (2002)

Nomura M、Kurita-Taniguchi M、Kondo K 等人：“小鼠巨细胞病毒 (CMV) 感染中补体的宿主细胞保护机制：CD46 启动子区域中 CMV 响应元件的鉴定”Eur J 免疫学杂志 32。 10. 2954-2964 (2002)

K.Kondo et al.: "Identification of human herpesvirus 6 latency-associated transcripts."J.Virol.. 76(8). 4145-1451 (2002)

K.Kondo 等人：“人类疱疹病毒 6 潜伏相关转录物的鉴定。”J.Virol.. 76(8)。

M.Nomura et al.: "Mechanism of host cell protection from complement in murine cytoiegalovirus (CMV) infection : identification of a CHV-responsive element in the CD46 promoter region."Eur J Immunol. 32(10). 2954-2964 (2002)

M.Nomura 等人：“小鼠细胞病毒 (CMV) 感染中补体对宿主细胞的保护机制：CD46 启动子区域 CHV 响应元件的鉴定。”Eur J Nutrition。

K.Shimada, K.Kondo, K.Yamanishi.: "Human Herpesvirus 6 Immediate-Early 2 Protein Interacts with Heterogeneous Ribonucleoprotein K and Casein Kinase 2"Microbiol.Immunol.. (in press).

K.Shimada、K.Kondo、K.Yamanishi.：“人类疱疹病毒 6 立即早期 2 蛋白与异质核糖核蛋白 K 和酪蛋白激酶 2 相互作用”Microbiol.Immunol..（印刷中）。