Analysis of epitopes for antibodies against oxidized lipoprotein and development of the assay system

虽然有越来越多的证据表明，在人类血清中的抗氧化LDL自身抗体，数据仍然相互矛盾。因此，我们使用凝胶渗透色谱法纯化的氧化LDL检测跟腱黄色瘤患者和对照组之间自身抗体滴度的差异。对15例高血压病合并跟腱黄瘤患者（A+组）和94例高血压病无黄瘤患者（A-组）进行了研究。使用ELISA方法进行抗氧化和抗天然LDL自身抗体的定量。为了计算抗体滴度，我们使用抗氧化和抗天然LDL威尔斯孔的分光光度阅读之间的比率。使用该技术，IgG水平在A+和A-组之间存在显著差异（p <0.01）。相比之下，使用天然和氧化LDL作为抗原，未经FPLC纯化，显示两组之间没有显着差异。此外，IgG自身抗体滴度与年龄、体重指数、总胆固醇、HDL胆固醇、LDL胆固醇或甘油三酯无显著相关性。基于这些结果，IgG自身抗体值似乎与跟腱黄色瘤相关。

Although there is increasing evidence of anti-oxidized LDL autoantibodies in human sera, the data remain contradictory. Thus, we examined the difference in autoantibody titers between patients with Achilles tendon xanthoma and control subjects using oxidized LDL that was purified by gel-permeation chromatography. Fifteen hyperlipidemic patients with Achilles tendon xanthoma (group A+) and 94 hyperlipidemic patients without Achilles tendon xanthoma (group A-) were studied. Quantification of anti-oxidized and anti-native LDL autoantibodies was performed using an ELISA method. To calculate antibody titers, we used the ratio between the spectrophotometric reading of anti-oxidized and anti-native LDL wells. Using this technique, IgG level differed significantly between groups A+ and A- (p < .01). In contrast using native and oxidized LDL as antigens without FPLC purification revealed no significant difference between the two groups. Furthermore, IgG autoantibody titer did not correlate significantly with age, body mass index, total cholesterol, HDL cholesterol, LDL cholesterol, or triglyceride. Based on these results, IgG autoantibody values appear to correlate with Achilles tendon xanthoma.