Analysis of epitopes for antibodies against oxidized lipoprotein and development of the assay system

尽管有越来越多的证据表明在人类血清中存在抗氧化LDL自身抗体，但数据仍然相互矛盾。因此，我们使用凝胶渗透层析纯化的氧化LDL检测跟腱黄瘤患者和对照组之间自身抗体滴度的差异。研究了15例高脂血症合并跟腱黄瘤（A+组）和94例无跟腱黄瘤的高脂血症（A-组）。采用ELISA法定量检测抗氧化和抗天然LDL自身抗体。为了计算抗体滴度，我们使用抗氧化和抗天然LDL孔的分光光度读数之比。A+组和A-组血清IgG水平差异有统计学意义（p < 0.01）。相比之下，未经FPLC纯化的天然LDL和氧化LDL作为抗原，两组之间没有显着差异。此外，IgG自身抗体滴度与年龄、体重指数、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇或甘油三酯没有显著相关性。基于这些结果，IgG自身抗体值似乎与跟腱黄瘤相关。

Although there is increasing evidence of anti-oxidized LDL autoantibodies in human sera, the data remain contradictory. Thus, we examined the difference in autoantibody titers between patients with Achilles tendon xanthoma and control subjects using oxidized LDL that was purified by gel-permeation chromatography. Fifteen hyperlipidemic patients with Achilles tendon xanthoma (group A+) and 94 hyperlipidemic patients without Achilles tendon xanthoma (group A-) were studied. Quantification of anti-oxidized and anti-native LDL autoantibodies was performed using an ELISA method. To calculate antibody titers, we used the ratio between the spectrophotometric reading of anti-oxidized and anti-native LDL wells. Using this technique, IgG level differed significantly between groups A+ and A- (p < .01). In contrast using native and oxidized LDL as antigens without FPLC purification revealed no significant difference between the two groups. Furthermore, IgG autoantibody titer did not correlate significantly with age, body mass index, total cholesterol, HDL cholesterol, LDL cholesterol, or triglyceride. Based on these results, IgG autoantibody values appear to correlate with Achilles tendon xanthoma.