The Mechanism for Formation and Maintenance of Synapses in the Prefrontal Cortex by Biogenic Amines

5-HT 2A受体介导5-HT在发育和成熟脑中形成和维持突触的功能。我们制备了抗5-HT_（2A）受体蛋白的特异性抗体。用该抗体检测了5-HT_（2A）受体在大鼠大脑皮层的分布。5-HT_（2A）受体免疫反应位于大脑皮层弥漫性分布。几乎所有的大脑皮质神经元都有免疫反应，尤其是体细胞和顶树突有较强的免疫反应。用GFAP抗体鉴定的星形胶质细胞显示强的5-HT_（2A）受体免疫反应。虽然存在的5-HT 2A受体在小脑之间的常规形态和功能的研究差异，我们的免疫组织化学研究表明，在浦肯野细胞的强烈的免疫反应。通过使用免疫电镜技术，5-HT 2A受体位于不对称突触的突触后膜厚度之下，可能是突触能的。为了阐明5-HT对谷氨酸能受体的调节机制，在6周龄大鼠大脑皮层中检测了5-HT耗竭一周后谷氨酸受体结合的变化。AMPA受体，特别是GluR 2上调。5-HT对突触可塑性的调节可能有两种途径：通过改变Quill受体亚型的组成来调节突触的数目和Ca ~（2+）的通透性。

5-HT2A receptor has been shown to mediate the function of 5-HT for formation and maintenance of synapses in the developing and mature brain. We raised specific antibodies against receptor protein of 5-HT2A receptor. By using the antibodies distribution pattern of 5-HT2A receptor was examined in the cerebral cortex of the rat. Immunoreaction of 5-HT2A receptor was located diffusely in the cerebral cortex. Virtually all the neurons in the cerebral cortex had the immunoreactivity, and specially somal profiles and apical dendrites had strong immunoreaction. Astrocyte identified by using an antibody GF AP showed a strong immunoreaction of 5-HT2A receptors. Although discrepancies in terms of exsistence of 5-HT2A receptors in the cerebellum between conventional morphological and functional studies, our immunohistochemical study has shown a strong immunoreaction in the Purkinje cells. By using an immuno- electron microscopical technique 5-HT2A receptor was located just beneath the postsynaptic membrane thicknings of asymmetrical synapses, possibly glutamatergic ones. To clarify regulatory mechanism for glutamatergic receptors by 5-HT changes in receptor bindings of glutamate were examined in the cerebral cortex 6 week-old rat after 5-HT depletion for a week. AMPA receptor, specially GluR2 was upregulated. 5-HT appears to regulate synaptic plasticity into two way : regulating the number of synapses and Ca permeability by changing composition of Quill receptor suntypes.