Lactic Acid Production from Kitchen Refuses for Biodegradable Plastics Production

利用厨房垃圾生产乳酸用于生产可生物降解塑料

• 批准号: 09450306
• 负责人: SHIRAI Yoshihito
• 金额: $ 8.26万
• 依托单位: Kyushu Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09450306/
• 关键词: kitchen refuses; lactic acid; biodegradable plastics; non-sterilized system; simulation; polyhydroxyalkanoate; Lactobacillus plantarum; Lactobacillus brevis; 開放系; シュミレーション; Lactobacillus plantarun; 乳酸菌; 混合培養; L.plantarum; L.hrevis; 大腸菌; クロ

The purpose of this project is to clarify the fundamentals for producing lactic acid from kitchen refuses by lactic acid bacteria who stay in the kitchen refuses under non-sterilized conditons. Lactic acid thus produced is to be used for the production of biodegradable plastics including polyhydroxyalkanoate (PHA) and poly-lactate. Lactic acid was efficiently produced under above conditions with a high electivity over 89% by adjusting pH at 7 intermittently every 12 hours ; the culture pH was swiftly dropped to 4 and returned rapidly to 7 by adding alkarine solution. It was suggested that the other bacteria than lactic acid bacteria contributed to the Ictic acid production by digesting starch to yield glucose and by supplying bitamines and other cofactors which assist the lactic acid bacterial growth. On the other hand, the harmful bacteria such as E-coli were ruined by intermittent p1-I drops. Lactobacillus plantarum and Lactobacillus brevis were found to be dominant bacteria. This was confirmed by a computer simulation.

本课题旨在阐明在非灭菌条件下，生活在厨房垃圾中的乳酸菌产乳酸的基本原理。由此生产的乳酸用于生产生物可降解塑料，包括聚羟基链烷酸酯（PHA）和聚乳酸酯。在上述条件下，通过每隔12小时间歇调节pH值至7，乳酸的产酸率可达89%以上，通过加入碱性溶液，pH值迅速降至4，又迅速恢复至7。结果表明，乳酸菌以外的细菌通过消化淀粉产生葡萄糖并通过提供氨和其他辅助因子来帮助乳酸菌生长，从而促进了乳酸的产生。另一方面，大肠杆菌等有害细菌被间歇性的p1-I下降所破坏。植物乳杆菌和短乳杆菌是优势菌。这一点得到了计算机模拟的证实。