Lactic Acid Production from Kitchen Refuses for Biodegradable Plastics Production

本项目的目的是澄清在未经消毒的条件下留在厨房垃圾中的乳酸菌从厨房垃圾中产生乳酸的基本原理。由此产生的乳酸将用于生产生物降解塑料，包括聚羟基烷酸酯（PHA）和聚乳酸。在上述条件下，通过每隔12 h间歇调节pH 7，乳酸的高效产率达到89%以上；加入碱性溶液后，培养液pH迅速降至4，又迅速恢复到7。结果表明，除乳酸菌外，其他细菌通过消化淀粉生成葡萄糖和提供辅助乳酸菌生长的双胺和其他辅助因子来促进乳酸菌的生成。另一方面，间歇性滴入p1-I可破坏大肠杆菌等有害细菌。植物乳杆菌和短乳杆菌为优势菌。计算机模拟证实了这一点。

The purpose of this project is to clarify the fundamentals for producing lactic acid from kitchen refuses by lactic acid bacteria who stay in the kitchen refuses under non-sterilized conditons. Lactic acid thus produced is to be used for the production of biodegradable plastics including polyhydroxyalkanoate (PHA) and poly-lactate. Lactic acid was efficiently produced under above conditions with a high electivity over 89% by adjusting pH at 7 intermittently every 12 hours ; the culture pH was swiftly dropped to 4 and returned rapidly to 7 by adding alkarine solution. It was suggested that the other bacteria than lactic acid bacteria contributed to the Ictic acid production by digesting starch to yield glucose and by supplying bitamines and other cofactors which assist the lactic acid bacterial growth. On the other hand, the harmful bacteria such as E-coli were ruined by intermittent p1-I drops. Lactobacillus plantarum and Lactobacillus brevis were found to be dominant bacteria. This was confirmed by a computer simulation.