Comparative study of human and chimpanzee chromosomes by fluorescence in situ hybridization
荧光原位杂交对人类和黑猩猩染色体的比较研究
基本信息
- 批准号:07640949
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Human chromosome 2 is the only obvious exception from the chromosomal homologies between the human karyotype and that of the great apes (Wienberg et al.1990,1992). Human chromosome 2 has been described that it arose out of the fusion of two ancestral ape chromosomes on the basis of comparisons of chromosome banding patterns between human and great apes. This has been confirmed by molecular data ; human 2q12-q14 region is homologous with chimpanzee (Pan troglodytes, PTR) 12p and 13p by comparative chromosome mapping with DNA microlibraries. Furthermore, the telomere-to-telomere fusion point has been conjectured to be localized in chromosomal band 2q13. Recently a high resolution cytogenetic map of human chromosome 2 with 434 cosmid makers was constructed by Takahashi et al. (1995). The present report describes the physical order of cosmid makers in the region 2q12-14 using FISH.The cosmids were labeled with either biotin-14-dATP or digoxigenin-11-dUTP by nick-translation. Two-color FISH for ordering was performed on metaphase chromosomes decondensed by actinomycin D and on DNA fibers. Preparation of DNA fibers was our modifications of the standard protocol (Suto et al., 1996). Totally 36 cosmids were localized on human and chimpanzee chromosomes. Seventeen human chromosome 2-originated markers were located to PTR 12 short arm, and nineteen to PTR 13 short arm. The order of clones on chimpanzee chromosomes was identical to that of human chromosome 2. The results suggest that human chromosome 2 arose out of tandem fusion between two ancestral ape-type chromosomes, and that the organization of the ancestral genome has been considerably conserved. Comparative mapping of this region among great apes may contribute to the dating of chromosomal reorganization in evolution.
人类 2 号染色体是人类核型与类人猿染色体核型之间唯一明显的例外(Wienberg 等,1990,1992)。根据人类和类人猿染色体带型的比较,人类 2 号染色体被描述为由两条祖先猿染色体融合而成。这已被分子数据证实;通过与 DNA 微文库进行比较染色体作图,人类 2q12-q14 区域与黑猩猩 (Pan troglodytes,PTR) 12p 和 13p 同源。此外,端粒到端粒的融合点被推测位于染色体带 2q13 中。最近,Takahashi 等人构建了具有 434 个粘粒标记的人类 2 号染色体的高分辨率细胞遗传学图谱。 (1995)。本报告使用 FISH 描述了 2q12-14 区域粘粒制造者的物理顺序。通过切口平移用生物素-14-dATP 或地高辛-11-dUTP 标记粘粒。对放线菌素 D 解凝的中期染色体和 DNA 纤维进行双色 FISH 排序。 DNA 纤维的制备是我们对标准方案的修改(Suto 等人,1996)。共有36个粘粒定位于人和黑猩猩的染色体上。 17 个人类 2 号染色体起源标记位于 PTR 12 短臂,19 个位于 PTR 13 短臂。黑猩猩染色体上的克隆顺序与人类 2 号染色体的顺序相同。结果表明,人类 2 号染色体是由两条祖先猿型染色体之间的串联融合产生的,并且祖先基因组的组织相当保守。类人猿这一区域的比较绘图可能有助于确定进化过程中染色体重组的年代。
项目成果
期刊论文数量(21)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
M.Hirai: "Topics in the studies of human chromosome evolution (in Japanese)" Anthrop.Science. 104. 355-364 (1996)
M.Hirai:“人类染色体进化研究主题(日语)”Anthrop.Science。
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M.Hirai: "Assignment of human ADP ribosylation factor(ARF)genes ARF1 and ARF3 to chromosome 1q42 and 12q13,respectively." Genomics,. vol.34. 263-265 (1996)
M.Hirai:“人类 ADP 核糖基化因子 (ARF) 基因 ARF1 和 ARF3 分别分配到染色体 1q42 和 12q13。”
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- 影响因子:0
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M.Hirai and Y.Suto: "Comparative mapping and human chromosome evolution (in Japanese)." Protein, Nuc.Acid, Enz.41. 285-293 (1996)
M.Hirai 和 Y.Suto:“比较作图和人类染色体进化(日语)”。
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- 影响因子:0
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Y.Suto: "Chromosomal localization of the human natural killer cell class 1 receptor family genes to 19q13.4 by fluorescence in situ hybridization." Genomics. vol.35. 270-274 (1996)
Y.Suto:“通过荧光原位杂交将人类自然杀伤细胞 1 类受体家族基因染色体定位到 19q13.4。”
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- 影响因子:0
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Y.Suto: "Visual demonstration of the human complement C4 and 21-hydroxylase genes by high-resolution fluorescence in situ hybridization." Genomics. vol.33. 321-324 (1996)
Y.Suto:“通过高分辨率荧光原位杂交直观展示人类补体 C4 和 21-羟化酶基因。”
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- 影响因子:0
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HIRAI Momoki其他文献
HIRAI Momoki的其他文献
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{{ truncateString('HIRAI Momoki', 18)}}的其他基金
Population Cytogenetic and Anthropological Research on Emvironmental Pollution in Manila.
马尼拉环境污染的人口细胞遗传学和人类学研究。
- 批准号:
13575016 - 财政年份:2001
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of 5'-end Sequences of Full-length cDNAs obtained from Chimpanzee Skin Tissues.
对从黑猩猩皮肤组织获得的全长 cDNA 的 5 端序列进行分析。
- 批准号:
13554035 - 财政年份:2001
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular cytogenetic study of species-specific chromosomal regions
物种特异性染色体区域的分子细胞遗传学研究
- 批准号:
10554051 - 财政年份:1998
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Population Genetic Study of Indigenous Peoples of Wallacea
华莱士原住民的群体遗传学研究
- 批准号:
10041158 - 财政年份:1998
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular Cytogenetic Study of the Tarsier
眼镜猴的分子细胞遗传学研究
- 批准号:
09440282 - 财政年份:1997
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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