Establishment of molecular biological diagnostic strategies for periodontal diseases

在这一研究中，我们试图建立一个战略来诊断周期性疾病的分子病理学。基因组聚合酶链反应（PCR）技术使我们能够敏感地和特别地检测卟啉单胞菌（P.G.）Actinobucillus actinomycetemcomitans （A.A.）在地下牙科广场上。Furthermore，实用反向转录（RT）-PCR技术，我们建立了一个用针生物学方法孤立的多细胞因子mRNA的半定量检测系统。然后，我们收集了成人和早期间歇期（EOP）患者在第一次访问、评估和支持期治疗（SPT）的时间内分析的那些样本，并根据分子生物技术描述描述分析。我们观察到了炎症性周期性疾病中一种不同的炎症细胞因子的上调mRNA表达。有趣的是，我们可以统计地将多个细胞分开 ... More 通过聚类分析将OKine mRNA表达概况纳入5组。在具体情况下，大约50%的退化站点从EOP被归类为组显示IL-8和IFN D2 γ D2 mRNA相对较低的表达。在另一项研究中，IgG亚类抗体的血清水平反对P. g。在5年的时间里，对Alveolar骨骼丢失的关系进行了审查。在SPT患者中，IgG 2水平和骨骼损失分数之间的关系出现了统计上显著的积极相关性（p<0.001）。本建议在观察到的持续IgG 2反应后可能与经常或持续的周期破坏有关。在体外研究中，我们演示了银杏叶外延细胞最好由P.G.秘密刺激的IL-8和MCP-1。Sonic Extract。在补充中，我们澄清了淋巴细胞和银杏纤维碎片之间的不良相互作用，并在银杏纤维碎片中诱导了炎症细胞因子表达。这些结果表明，那些居住细胞在炎症周期性疾病中活跃地参与细胞因子网络的结果。Less（低）

In this study, we attempted to establish the strategies to diagnose the molecular pathogenesis of periodontal diseases. Genomic polymerase chain reaction (PCR) technique enabled us to sensitively and specifically detect Porphyromonas gingivalis (P.g.) and Actinobucillus actinomycetemcomitans (A.a.) in subgingival dental plaques. Furthermore, utilizing reverse transcription (RT)-PCR technique, we established the semiquantitative detection system of multiple cytokine mRNA in gingival specimens isolated by needle biopsy method. We then collected subgingival dental plaque, gingival specimens and serum from adult and early-onset periodontitis (EOP) patients at the times of first visit, reevaluation and supportive periodontal therapy (SPT) and analyzed those samples by molecular biological techniques described above. We observed the upregulated mRNA expression of a variety of inflammatory cytokines in inflamed periodontal lesions. Interestingly, we could statistically divide the multiple cyt … More okine mRNA expression profiles into 5 groups by cluster analysis. In particular, approximately 50% of the diseased sites from EOP were categorized into the groups showing relatively low expression of IL-8 and IFNィイD2γィエD2 mRNA.In another study, serum levels of IgG subclass antibodies against P.g. were examined in relation to alveolar bone loss in periodontitis patients receiving SPT for 5 years. Statistically significant positive correlation was seen for the relationship between IgG2 levels and △bone loss score (p<0.001) in the SPT patients. This suggests that the prolonged IgG2 response observed after periodontal treatment may be associated with recurrent or persistent periodontal destruction.In in vitro studies, we demonstrated that gingival epithelial cells preferentially secreted IL-8 and MCP-1 when stimulated with P.g. sonic extract. In addition, we clarified that adhesive interactions between lymphocytes and gingival fibroblasts induced inflammatory cytokine expression in the gingival fibroblasts. These results suggest that those resident cells actively participate in the cytokine network in inflamed periodontal lesions. Less