Establishment of evidence based antibacterial chemotherapy

建立循证抗菌化疗

• 批准号: 13671651
• 负责人: OKADA Hiroshi
• 金额: $ 2.3万
• 依托单位: Teikyo University (2003-2004)Kobe University (2001-2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671651/
• 关键词: UTI; Susceptihility; Bacteria; 薬剤感受性試験; MIC; 細菌数定量測定; 尿路感染症; フローサイトメトリー; 抗菌化学療法; 尿中細菌数; 自動測定; 定量測定; 尿中赤血球; 尿中白血球

A new automated flow cytometry based urine bacteria analyzer, UBA, was developed. We assessed the UBA for linearity of measurement, reproducibility of results, carryover rate, and correlation of the measured results with those determined by urine culture. We also evaluated for its ability to screen urine samples for significant bacteriuria.The UBA showed excellent linearity and minor carryover rate. Results from the UBA were highly reproducible, and in between-run precision assays, the coefficients of variation for UBA results were smaller than those by the urine culture.Two hundred and seventy-three urine specimens from patients attending outpatient clinic of university based hospital were examined. The results from the UBA were compared with those from urine culture.The UBA detected significant bacteria with sensitivity 96.6%, specificity 79.9%, positive predictive value 57.0%, negative predictive value 98.8%, false positive rate 15.8%, false negative rate 0.7%, and accuracy 83.5%. These results were comparable to or better than those obtained with previously reported screening procedures.The UBA can perform accurate enumeration of bacterial cells automatically in 90 seconds, and can be used for the screening of significant bacteriuria. Moreover, the UBA can be used to develop a new device to determine the susceptibilities of bacteria in urinary tract infections.

研制了一种新型尿细菌自动分析仪乌巴。我们评估了乌巴的测量线性、结果重现性、携带率以及测量结果与尿培养测定结果的相关性。我们还评价了它筛查尿中细菌的能力，乌巴显示出良好的线性和轻微的携带污染率。乌巴结果重复性好，批间精密度试验中，乌巴结果的变异系数小于尿培养结果。乌巴与尿培养结果比较，乌巴检出有意义细菌的敏感性为96.6%，特异性为79.9%，阳性预测值为57.0%，阴性预测值为98.8%，假阳性率为15.8%，假阴性率为0.7%，准确性为83.5%。这些结果与以前报道的筛选程序相当或更好。乌巴可以在90秒内自动进行准确的细菌细胞计数，并可用于筛选重要的菌尿。此外，乌巴可用于开发一种新的装置，以确定尿路感染中细菌的亲和性。

项目成果

Enumeration of bacterial cell numbers and detection of significant bacteriuria by a new flow cytometry-based device.

通过新型流式细胞术设备计数细菌细胞数并检测显着菌尿。

• 期刊: Am.J.Clin.Pathol. (Submitted for publication)
• 作者: Hiroshi Okada;Toshiro Shirakawa;Katsumi Shigemura;Kazushi Tanaka;Soichi Arakawa;Akinobu Gotoh;Yukio Hamaguchi;Sadao Kamidono
• 通讯作者: Sadao Kamidono

Enumeration of bacterial cell numbers and detection of significant bacteriuria by new flow cytometry-based device.

通过基于流式细胞术的新型装置对细菌细胞数进行计数并检测显着菌尿。

• 期刊: Am.J.Clin.Pathol. (Submitted for publication)
• 作者: Hiroshi Okada;Toshiro Shirakawa;Katsumi Shigemura;Kazushi Tanaka;Soichi Arakawa;Akinobu Gotoh;Yukio Hamaguchi;Sadao Kamidono
• 通讯作者: Sadao Kamidono

Hiroshi Okada: "Evaluation of the Sysmex UF-50"Am.J.Clin Pathol.. 115. 605-610 (2001)

Hiroshi Okada：“Sysmex UF-50 的评估”Am.J.Clin Pathol.. 115. 605-610 (2001)