Immunological Study on the Establishment of Human Chronic Periapical Periodontitis

最近的观察表明，人类根尖周病变的发展作为一个结果，免疫病理反应的连续抗原刺激感染根管。本研究的目的是阐明免疫机制参与根尖周病变的发病机制。首先，我们调查的属性感染根管内容物从临床的角度来看，在抗原性。提示根管内容物的湿润状态、黑色色素沉着、恶臭等性质与根尖周病变的存在密切相关。这些结果表明，细菌可能是至关重要的抗原物质在运河内容物。因此，根管中的微生物进行了调查。结果表明，包括类杆菌在内的革兰氏阴性杆菌与根尖周病变的存在密切相关。因此，厌氧性革兰氏阴性杆菌在根尖周发育中作为抗原刺激物是重要的 ...更多信息 其次，我们用免疫组织化学方法对根尖周病变中的免疫活性细胞进行了表征，并研究了它们与临床表现的相关性。结果表明，这些免疫活性细胞的比例与一些临床表现有关。最后，我们采用酶联免疫吸附法（ELISA）检测了感染根管根尖周渗出液中免疫球蛋白和白细胞介素-1（IL-1）的含量，并分析了这些免疫组分与临床表现的相关性。结果表明，根尖周渗出液中IL-1和免疫球蛋白的检测与临床有关。此外，随着尖周病变的愈合，IL-1水平升高，IL-1、IgG和伊加水平降低。因此，根尖周病变的免疫反应因病变的不同而不同，提示免疫反应在根尖周病变的发病中起重要作用。少

Recent observations suggested that human periapical lesions developed as a result of immunopathological responses to continuous antigenic stimuli from infected root canals. The purpose of this study was to clarify the involvement of immune mechanisms in the pathogenesis of periapical lesions.First, we investigated the property of infected root canal contents from a clinical point of view in terms of antigenicity. It revealed that some qualities of root canal contents, namely wet condition, black pigmentation and foul odors, were closely related to existence of periapical lesions. These findings suggested that bacteria might be the crucial antigenic substance in the canal contents. Therefore microorganisms in the root canals were investigated. Results showed anaerobic gram-negative rods including bacteroides species had closely relation to the existence of periapical lesions. Consequently, anaerobic gram-negative rods were important as the antigenic stimuli in development of periapical … More lesions.Second, we characterized the immunocompetent cells in periapical lesions by using immunohistochemical methods, and examined their correlation with clinical findings. Results showed the proportion of these immunocompetent cells related to some clinical findings. These observations suggested that immune responses closely related to the pathosis of periapical lesions.Finally, we measured the concentration of immunoglobulins and Interleukin-1(IL-1) in the periapical exudates of infected root canals by enzyme-linked immunosorbent assay and examined the correlation of these immune components to clinical findings. Results showed that IL-1 and immunoglobulines detected in periapical exudates were related with some clinical findings. Moreover, as the healing of periapical lesions, an increase of the level of IL-1 and a decrease of the levels of IL-1 , IgG and IgA were observed. Consequently immune responses in periapical lesions varied according to the pathosis of periapical lisions.Above findings suggested immune responses played a important role in the pathogenesis of the periapical lesions. Less