Influence of herpesvirus in infection/reactivation to AIDS

研究了人类免疫缺陷病毒（HIV）血清阳性母亲所生227例儿童中人类疱疹病毒6 （HHV6）感染的作用。41名hiv未感染婴儿中，3名（7%）在出生后第一个月HHV6 DNA阳性。提示可能有宫内感染。hiv感染儿童6个月和12个月时HHV6的累积感染率（分别为11%和33%）显著低于未感染儿童（28%和78%）。分别;P <措施)。HHV6感染前CD4+细胞数量高（约15%）与HHV6感染率高存在相关性。研究了22名被疾病控制和预防中心分类为N1或N2期的HIV婴儿HHV6感染与HIV疾病进展的关系。其中10名婴儿患有HHV6病毒，12名没有。在未感染HHV6病毒的婴儿中，有5名（42%）在1岁前未出现HIV疾病进展；然而，在所有10名感染HHV6病毒的儿童中，HIV疾病都有进展。研究结果表明，HHV6感染与纵向HIV感染儿童的HIV疾病进展有关（P< 0.05）。(J.Infect。调查了泰国有或无皮肤疾病的人类免疫缺陷病毒1型（HIV1）携带者和HIV1型阴性人群卡波西肉瘤相关疱疹病毒（KSHV）或人类疱疹病毒8 （HHV8）的血清阳性率。在有或无皮肤疾病的hiv - 1携带者中，KSHV/HHV8裂解抗原血清阳性率分别为25%和7.4%，而在hiv - 1阴性个体中无抗体。在CD4/CD8比值较低的HIV阳性个体中，KSHV/HHV8抗原的血清阳性率较高，提示KSHV/HHV8在免疫抑制状态下被重新激活。我们的研究结果表明，KSHV/HHV8在HIV携带者中与HIV共存，HHV8的存在可能与皮肤特征有关。用KSHV/HHV8病毒vIRF特异性抗体对vIRF （K9）基因及其产物进行了鉴定。vIRF是早期在细胞核中表达的基因，在功能上与细胞IRF1起作用，提示vIRF可能参与卡波西肉瘤的肿瘤形成。对HHV7 R2重复序列进行表征和分析，验证其传播状态。R2重复数被证明是鉴定传播源的良好标记。另一方面，糖蛋白H （gH）和糖蛋白B （gB）在15株分离株中表现出较高的相似性，表明这些结构基因不适合作为亚型标记。少

The role of human herpesvirus 6 (HHV6) infection in 227 children born to human immunodeficiency virus (HIV)-seropositive mothers was investigated. Of 41 HIV-unifected infants, 3(7%) were positive for HHV6 DNA in the first morth of life. suggesting possible intrauterine infection. The cumulative infection rates of HHV6 at 6 and 12 months of age were significantly lower in HIV-infected children (11% and 33%, respectively) than in uninfected children (28% and 78%. respectively ; P<.001). There was an association between high CD4+ cell numbers (>15%) before HHV6 infection and high HHV6 infection rate. Twenty two infants with HIV classed as Centers for Disease Control and Prevention stages N1 or N2 were studied for an association of HHV6 infection with progression of HIV disease. Ten of the infants had HHV6, and 12 did not. In 5 of the infants without HHV6 (42%), HIV disease had not progressed by 1 year of age ; however, HIV disease had progressed in all 10 children with HHV6 infection. the … More ses results suggest an association of HHV6 infection and progression of HIV disease in the study children with vertical HIV1 infection (P<.05). (J.Infect. Disease, 180 : 50-55, 1999)The seroprevalence to Kaposi's sarcoma-assocciated herpesvirus (KSHV) or human herpesvirus8 (HHV8) was surveyed in human immunodeficiency virus type 1 (HIV1) carriers with or without skin diseases, and also in HIV1 negative individuals in Thailand. With IFA, the seropositive rates to lytic antigens of KSHV/HHV8 in HIV1 carriers with or without skin diseases were 25% and 7.4%, respectively, but none of HIV1 negative individuals ahd antibody. The seroprevalence to KSHV/HHV8 antigens was high in HIV positive individuals with low CD4/CD8 ratio, suggesting that KSHV/HHV8 is reactivated during the immunosuppressive state. Our results suggested that KSHV/HHV8 co-existed with HIV in HIV1 carriers and the existence of HHV8 may be associated with features in the skin.vIRF (K9) gene and its product was characterized by using a specific antibody against vIRF of KSHV/HHV8. vIRF was an early gene expressed in the nucleus and functionally worked against the cellular IRF1, suggesting that vIRF may be involved in Kaposi's sarcoma tumor formation.R2 repeat of HHV7 was characterized and analyzed to verify the transmission status. R2 repeat number was proved to be a good marker for the identification of the origin of transmission. On the other hand, glycoprotein H (gH) and glycoprotein B (gB) showed high similarity among fifteen isolates, suggesting that these structural genes were not suitable for the subtype marker. Less