Influence of herpesvirus in infection/reactivation to AIDS

疱疹病毒对艾滋病感染/再激活的影响

• 批准号: 09041183
• 负责人: YAMANISHI Koichi
• 金额: $ 5.95万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09041183/
• 关键词: HIV; HHV6; HHV7; HHV8; reactivation; latentinfection; IFA; vIRF(K9); BC3; HIV; BCBL1; LANA; IFA; ヒトヘルペスウイルス6(HHV-6); ヒトヘルペスウイルス7(HHV-7); ヒトヘルペスウイルス8(HHV-8); ヒト免疫不全ウイルス(HIV); 先天性HIV感染; 血清疫学; 皮膚疾患; EBウイルス(EBV); 抗HHV-8抗体

The role of human herpesvirus 6 (HHV6) infection in 227 children born to human immunodeficiency virus (HIV)-seropositive mothers was investigated. Of 41 HIV-unifected infants, 3(7%) were positive for HHV6 DNA in the first morth of life. suggesting possible intrauterine infection. The cumulative infection rates of HHV6 at 6 and 12 months of age were significantly lower in HIV-infected children (11% and 33%, respectively) than in uninfected children (28% and 78%. respectively ; P<.001). There was an association between high CD4+ cell numbers (>15%) before HHV6 infection and high HHV6 infection rate. Twenty two infants with HIV classed as Centers for Disease Control and Prevention stages N1 or N2 were studied for an association of HHV6 infection with progression of HIV disease. Ten of the infants had HHV6, and 12 did not. In 5 of the infants without HHV6 (42%), HIV disease had not progressed by 1 year of age ; however, HIV disease had progressed in all 10 children with HHV6 infection. the … More ses results suggest an association of HHV6 infection and progression of HIV disease in the study children with vertical HIV1 infection (P<.05). (J.Infect. Disease, 180 : 50-55, 1999)The seroprevalence to Kaposi's sarcoma-assocciated herpesvirus (KSHV) or human herpesvirus8 (HHV8) was surveyed in human immunodeficiency virus type 1 (HIV1) carriers with or without skin diseases, and also in HIV1 negative individuals in Thailand. With IFA, the seropositive rates to lytic antigens of KSHV/HHV8 in HIV1 carriers with or without skin diseases were 25% and 7.4%, respectively, but none of HIV1 negative individuals ahd antibody. The seroprevalence to KSHV/HHV8 antigens was high in HIV positive individuals with low CD4/CD8 ratio, suggesting that KSHV/HHV8 is reactivated during the immunosuppressive state. Our results suggested that KSHV/HHV8 co-existed with HIV in HIV1 carriers and the existence of HHV8 may be associated with features in the skin.vIRF (K9) gene and its product was characterized by using a specific antibody against vIRF of KSHV/HHV8. vIRF was an early gene expressed in the nucleus and functionally worked against the cellular IRF1, suggesting that vIRF may be involved in Kaposi's sarcoma tumor formation.R2 repeat of HHV7 was characterized and analyzed to verify the transmission status. R2 repeat number was proved to be a good marker for the identification of the origin of transmission. On the other hand, glycoprotein H (gH) and glycoprotein B (gB) showed high similarity among fifteen isolates, suggesting that these structural genes were not suitable for the subtype marker. Less

本文对227例HIV阳性母亲所生儿童进行了人类疱疹病毒6型（HHV 6）感染的调查。在41例未感染HIV的婴儿中，3例（7%）在出生后第一个月内HHV 6 DNA阳性。可能是宫内感染在6个月和12个月时，HIV感染儿童的HHV 6累积感染率（分别为11%和33%）显著低于未感染儿童（28%和78%）。P<0.001）。HHV 6感染前CD 4+细胞数高（>15%）与HHV 6感染率高有关。研究了22名被疾病控制和预防中心归类为N1或N2期的HIV感染婴儿的HHV 6感染与HIV疾病进展的相关性。其中10名婴儿有HHV 6，12名没有。在5名没有HHV 6的婴儿（42%）中，HIV疾病在1岁时没有进展;然而，在所有10名HHV 6感染的儿童中，HIV疾病都有进展。的 ...更多信息 研究结果表明，在垂直HIV 1感染的研究儿童中，HHV 6感染与HIV疾病进展相关（P<0.05）。（J.J.第180章：50-55，1999）在泰国，在有或没有皮肤病的人类免疫缺陷病毒1型（HIV 1）携带者中，以及在HIV 1阴性个体中，调查了卡波西肉瘤相关疱疹病毒（KSHV）或人类疱疹病毒8（HHV 8）的血清阳性率。用IFA法检测有皮肤病和无皮肤病的HIV-1携带者KSHV/HHV-8裂解抗原的阳性率分别为25%和7.4%，而HIV-1阴性者无一阳性。KSHV/HHV 8抗原的血清阳性率在具有低的CD 4/CD 8比率的HIV阳性个体中是高的，这表明KSHV/HHV 8在免疫抑制状态期间被重新激活。结果表明，KSHV/HHV 8与HIV在HIV 1携带者中共存，HHV 8的存在可能与皮肤特征有关。vIRF是一个在细胞核中表达的早期基因，其功能与细胞内的IRF 1相反，提示vIRF可能参与了Kaposi肉瘤的形成。R2重复数是鉴定传播源的良好标记。另一方面，糖蛋白H（gH）和糖蛋白B（gB）在15个分离物中表现出较高的相似性，表明这些结构基因不适合作为亚型标记。少

项目成果

Nishimoto S, Inagi R, Yamanishi K et al.: "Prevalence of human herpesvirus-8 in skin lesions." Br J Dermatol.137(2). 179-184 (1997)

Nishimoto S、Inagi R、Yanishi K 等人：“皮肤损伤中人类疱疹病毒 8 型的流行情况。”

Kositanont, U., Wasi, C., Wanprapar, N., Bowonkiratikachorn, P., Chokephaibulkit, K., Chearskul, S., Chimabutra, K., Sutthent, R., Foongladda, S., Inagi, R., Kurata, T., and Yamanishi, K.: "Primary infection of human herpesvirus 6 in children with vertica

Kositanont, U.、Wasi, C.、Wanprapar, N.、Bowonkiratikachorn, P.、Chokephaibulkit, K.、Chearskul, S.、Chimabutra, K.、Sutthent, R.、Foongladda, S.、Inagi, R.,

Mukai.T, Hata.A, Isegawa.Y et al.: "Characterization of glycoprotein H and L of human herpesvirus 7." Microbiol Immunol.41(1). 43-50 (1997)

Mukai.T、Hata.A、Isekawa.Y 等人：“人类疱疹病毒 7 型糖蛋白 H 和 L 的表征。”

R.Inagi, et al.: "Identification and characterization of human herpesvirus 8 (HHV-8) ORF K9 (viral interferon regulatory factor: vIRF) by a monoclonal antibody." J.Human Virol.(in press).

R.Inagi 等人：“通过单克隆抗体对人疱疹病毒 8 (HHV-8) ORF K9（病毒干扰素调节因子：vIRF）进行鉴定和表征。”