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Studies on the mechamism of latency and reactivation of beta-herpesviruses

β-疱疹病毒潜伏和再激活机制的研究

基本信息

批准号：
06304026
负责人：
YAMANISHI Koichi
金额：
$ 11.46万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

Herpesviruses (CMV,HHV-6 and HHV-7), which belong to beta-herpesvirus subfamily, infect latently in vivo after the primary infection. However the mechanism of latency and its reactivation is not clear yet. Minamishima et al.reported the seroprevalence of CMV in pregnant women in Japan, suggesting the CMV infection in Japan is still high and different from that in Europe and USA.Hirai et al.reported that the gene products of UL112/113 of CMV,which belong to early gene, are essential for DNA replication, and the appearance of antibody to pp34 correlated with the clinical symptoms after the renal transplantation. Nishiyama et al reported that the H strand of MHC class I was synthesized normally in CMV-infected cells but the transport if MHC from RES to Golgi apparatus was inhibited in infected cells. Therefore, MHC can not reach the cell membrane. Tsutsui et al.have been developing the transgenic mice by transfecting a immediate early gene of mouse CMV and trying to use it as a human model. Ihara et al.have developed humanized monoclonal antibodies which neutrakize HCMV and are going to use them for treatment of CMV infection after the organ transplantation. Shiraki et al.found anti-CMV agents in the extract of medicinal plants. Kurata et al.developed the latent system of HHV-6A in a retinal pigment epithelial cell line. Nii et al.found that HHV-7 infects persistenly in SupT1 cells. Yamanishi et al.compared the detection rate of HHV-6 and HHV-7 in saliva of healthy children by age, suggesting that HHV-7 was highly secreted even in adults. Furthemore, they analyzed an immediate early gene which may an important role for the regulation of HHV-6 gene expression.

疱疹病毒（CMV、HHV-6和HHV-7）属于β-疱疹病毒亚科，初次感染后在体内潜伏感染。然而，潜伏期及其重新激活的机制尚不清楚。 Minamishima等报道日本孕妇CMV血清阳性率，提示日本CMV感染率仍较高，与欧美不同。Hirai等报道CMV的UL112/113基因产物属于早期基因，对DNA复制至关重要，pp34抗体的出现与肾病后临床症状相关。移植。 Nishiyama等人报道，MHC I类的H链在CMV感染的细胞中正常合成，但MHC从RES到高尔基体的运输在感染的细胞中受到抑制。因此，MHC无法到达细胞膜。 Tsutsui等人通过转染小鼠CMV的早期基因来开发转基因小鼠，并尝试将其用作人类模型。 Ihara等人开发了中和HCMV的人源化单克隆抗体，并将用它们来治疗器官移植后的CMV感染。 Shiraki等人在药用植物提取物中发现了抗CMV药物。 Kurata 等人在视网膜色素上皮细胞系中开发了 HHV-6A 的潜在系统。 Nii等人发现HHV-7在SupT1细胞中持续感染。 Yamanishi等人按年龄比较了健康儿童唾液中HHV-6和HHV-7的检出率，表明即使在成人中，HHV-7也高度分泌。此外，他们分析了一个可能在 HHV-6 基因表达调控中发挥重要作用的早期基因。