Study on pathogenesis of Human herpesvirus 6 and 7 infection

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1) Analysis of HHV-6 and HHV-7 DNA ; About 90% of total HHV-6 DNA (HST strain) and 10% of HHV-7 DNA (KHR strain) was sequenced during this period. When their sequences were compared with those of HHV-6A and human cytomegalovirus which were already published elsewhere by computer, 95% of DNA homology between HHV-6A and B was found, but less homologous regions were also observed. Next, when the homology search was done in regions of major capsid antigen of HHV-6 and HHV-7, the homology was approximately 65%. But it was more homologous than other human herpesviruses.2) Analysis of HHV-6 and 7 proteins ; we have been collecting monoclonal antibodies against HHV-6 and HHV-7, and we could obtain more than 50 antibodies to HHV-6A and B,and 42 monoclonal antibodies to HHV-7. Although they were virus specific ones, and some have neutralizing activities, others cross-reacted with HHV-6 and HHV-7.3) Latency and reactivation of HHV-6 ; we developed in vitro latency system, and HHV-6 infects latently in monocytes/macrophages. We also found that HHV-6 was reactivated during other virus infection such as HHV-7 or dengue virus.