Study on pathogenesis of Human herpesvirus 6 and 7 infection

人类疱疹病毒6型和7型感染发病机制的研究

• 批准号: 04404033
• 负责人: YAMANISHI Koichi
• 金额: $ 16万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04404033/
• 关键词: HHV-6; HHV-7; HCMV; immediate early; Herpesvirus; Glycoprotein; latency; reactivation; 前初期タンパク; US22family; クローニング; モノクローン抗体; DNA; シークエンス; 初期タンパク; PCR

1) Analysis of HHV-6 and HHV-7 DNA ; About 90% of total HHV-6 DNA (HST strain) and 10% of HHV-7 DNA (KHR strain) was sequenced during this period. When their sequences were compared with those of HHV-6A and human cytomegalovirus which were already published elsewhere by computer, 95% of DNA homology between HHV-6A and B was found, but less homologous regions were also observed. Next, when the homology search was done in regions of major capsid antigen of HHV-6 and HHV-7, the homology was approximately 65%. But it was more homologous than other human herpesviruses.2) Analysis of HHV-6 and 7 proteins ; we have been collecting monoclonal antibodies against HHV-6 and HHV-7, and we could obtain more than 50 antibodies to HHV-6A and B,and 42 monoclonal antibodies to HHV-7. Although they were virus specific ones, and some have neutralizing activities, others cross-reacted with HHV-6 and HHV-7.3) Latency and reactivation of HHV-6 ; we developed in vitro latency system, and HHV-6 infects latently in monocytes/macrophages. We also found that HHV-6 was reactivated during other virus infection such as HHV-7 or dengue virus.

1)HHV-6和HHV-7 DNA分析：在此期间，约90%的HHV-6 DNA（HST株）和10%的HHV-7 DNA（KHR株）被测序。与已发表的HHV-6A和人巨细胞病毒（HCMV）的DNA序列比较，HHV-6A和B的同源性达95%，但同源性较低。其次，当在HHV-6和HHV-7的主要衣壳抗原的区域中进行同源性搜索时，同源性约为65%。2）HHV-6和HHV-7蛋白的分析：我们一直在收集抗HHV-6和HHV-7的单克隆抗体，目前已经获得了50多株抗HHV-6A和B的单克隆抗体，以及42株抗HHV-7的单克隆抗体。3）HHV-6的潜伏和再激活：我们建立了体外潜伏系统，HHV-6在单核/巨噬细胞中潜伏感染。我们还发现HHV-6在其他病毒感染如HHV-7或登革病毒时被重新激活。

项目成果

SP.Adler et al: "Antibodies induced by a primary sytomegalovirus infection react with human herpesvirus 6 prptein" J. Infect. Dis.168. 1129-1126 (1993)

SP.Adler 等人：“原发性巨细胞病毒感染诱导的抗体与人疱疹病毒 6 蛋白发生反应”J. Infect。

K.Yamanishi et al: "Human herpesvirus 6 (HHV-6) infection in the central nervous system" Acta. Paediatr.34. 337-343 (1992)

K.Yamanishi 等人：“中枢神经系统中的人类疱疹病毒 6 (HHV-6) 感染”Acta。

T.Nakagami et al: "Antiviral activity of a bile pigment, biliverdin,against human herpesvirus 6 (HHV-6) in vitro" Microbiol. Immunol.36. 381-390 (1992)

T.Nakagami 等人：“胆汁色素胆绿素对人疱疹病毒 6 (HHV-6) 的体外抗病毒活性”微生物学。

S.Sato et al: "Identification of human herpesvirus 6 uracil-DNA glycosylase gene" J.Gen.Virol.75. 2349-2354 (1994)

S.Sato 等人：“人疱疹病毒 6 尿嘧啶-DNA 糖基化酶基因的鉴定”J.Gen.Virol.75。

K.Tanaka et al: "Human herpesvirus 7 : another causal agent for roseola (exanthem subitum)" J. Pediatr.125. 1-5 (1994)

K.Tanaka 等人：“人类疱疹病毒 7：玫瑰疹（急疹）的另一种病原体”J. Pediatr.125。