Role of Heat Shock Protein against Protozoan Infection

在HSP家族中，HSP 90已经给予了很大的关注。这种蛋白质是由形成复合体组成的细胞功能，包括许多细胞蛋白质，如激酶、磷酸酶、核激素受体、活性素、管蛋白和蛋白质组。水果油炸与HSP 90基因的同源突变无法存活，而那些与异质突变经常显示形态学异常现象。HSP 90的过度表达增强了该年酿酒酵母在老鼠中的病毒。我们研究了寄生虫的表达机制。HSP 90及其与疟疾寄生虫病毒的相关性HSP 90在P. Yoelii的L股感染的寄生虫中强烈地表达出来，并且只在L股感染的老鼠中略微地表达出来。有趣的是，没有在用抗CD 4和抗TcR-α或抗IFN-γ单克隆抗体中检测到HSP 90表达，但没有在用抗CD 8和抗TcR-治疗的寄生虫中检测到寄生虫中检测到。 ... More γδ或抗IL-4抗体。By using SCID mice transferred with CD4 y D1-y D1 or CD8 y D1 splenic cells of BALB/c， we demonstrated again that CD4 y D1+ y D1T cells but not CD8 y D1 + y D1T cells were indispensable for the expression of parasite HSP90.免疫电子显微镜分析证实，大规模HSP 90信号被表达在核分裂体和胶质细胞中，但仅略微在这些寄生虫中的细胞系细胞系，以及在甲状腺肿和胶质细胞中。反应性氧化物物种（ROS）和硝酸氧化物（NO），但不含IFN-γ治疗增加了HSP 90表达在寄生性文化中的表达。HSP 90表达被认为是明显地在较后一个阶段的感染与L型应变有关，可能是因为感染期间的免疫抑制。来自免疫成分老鼠的寄生虫比来自SCID老鼠的寄生虫感染性高，建议寄生虫表达HSP 90比寄生虫表达HSP 90的寄生虫感染性高。最后，P. Yoelii HSP 90的氨基酸序列是从cDNA序列中提取出来的。在融合中，寄生性HSP 90是一个由主机免疫反应诱导的病毒因素。在简短中，我们的结果表明，HSP 90似乎在宿主-寄生性交互中扮演关键角色。Less（低）

Among HSP families, HSP90 has been given much attention. This protein is involved in cellular functions by forming complexes with many cellular proteins such as kinases, phosphatases, nuclear hormone receptors, actin, tubulin, and the proteasome. Fruit flies with homozygous mutations of HSP90 gene cannot survive and those with heterozygous mutation frequently show morphological abnormalities. Over-expression of HSP90 enhances the virulence of the yeast Saccharomyces cerevisiae in mice. We have studied the expression mechanism of parasite. HSP90 and its correlation with virulence of murine malaria parasite. HSP90 was strongly expressed in parasite from B6 mice infected with the L strain of P. yoelii and only slightly expressed in parasite from mice infected with the L strain. Interestingly, HSP90 expression was not detected in parasites from SCID mice treated with anti-CD4 and anti-TcR-αβ or anti-IFN-γmonoclonal antibodies, but not in parasite from those treated with anti-CD8, anti-TcR- … More γδ or anti-IL-4 antibody. By using SCID mice transferred with CD4ィイD1-ィエD1 or CD8ィイD1-ィエD1 splenic cells of BALB/c, we demonstrated again that CD4ィイD1+ィエD1 T cells but not CD8ィイD1+ィエD1 T cells were indispensable for the expression of parasite HSP90. Immunoelectron microscopic analysis confirmed that massive HSP90 signal was expressed in nuclei of schizonts and merozoits but only slightly in cytoplasma of these parasites and in trophozoits and gametocytes. Reactive oxygen species (ROS) and nitric oxide (NO) but not IFN-γ treatment increased HSP90 expression in parasite cultured in vitro. HSP90 expression was decreased significantly at later period of infection with the L strain, possibly because of immune-suppression during the infection. Parasite from immune-component mice was highly infective than that from SCID mice, suggesting that parasites expressing HSP90 have higher infectivity than parasites not expressing HSP90. Finally, the amino acid sequence of P. yoelii HSP90 was deduced from the cDNA sequence. In conclusion, parasite HSP90 is a virulent factor and induced by host immune response.In brief, our results show that HSPs appear to play crucial roles in host-parasite interaction. Less