Microscopy Core

显微镜核心

• 批准号: 10155471
• 负责人: KENNETH W DUNN
• 金额: $ 17.12万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-06 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10155471
• 关键词: 20 year old; Anesthesia procedures; Animals; Basic Science; Biological; Biological Assay; Biosensor; Brain; Budgets; Cell physiology; Cellular biology; Collection; Confocal Microscopy; Contract Services; Data Storage and Retrieval; Development; Diabetes Mellitus; Ensure; Fluorescence; Fostering; Functional disorder; Goals; Grant; Hour; Human; Image Analysis; In Vitro; Indiana; Individual; Infrastructure; Islets of Langerhans Transplantation; Kidney; Knowledge; Laboratories; Liver; Manufacturer Name; Metabolic Diseases; Methods; Microscope; Microscopy; Mission; Modeling; Monitor; Mus; Muscle; Neurons; Operative Surgical Procedures; Pancreas; Performance; Physiology; Proteins; Reagent; Research; Research Personnel; Savings; Scanning; Schedule; Scientist; Services; System; Techniques; Three-Dimensional Image; Time; Training; Training Support; Translational Research; Validation; Viral Vector; Work; base; biological systems; capsule; cost; design; digital; digital imaging; discount; experience; fluorescence lifetime imaging; imaging approach; imaging modality; interest; intravital microscopy; islet; light microscopy; medical schools; microscopic imaging; multiphoton microscopy; novel; protein function; research study; technique development; tissue resource; two-dimensional; vector

ABSTRACT (MICROSCOPY CORE) The overall goal of the Microscopy Core is to provide IDRC investigators with effective access to advanced methods of light microscopy, particularly the powerful new techniques of intravital microscopy developed during the previous grant period. The Microscopy Core utilizes the extensive facilities of the Indiana Center for Biological Microscopy (ICBM), the School of Medicine's 20 year-old core microscopy facility. The Core is equipped with three point-scanning confocal/multiphoton excitation microscopes, as well as a spinning-disk confocal microscope and multiple digital workstations supporting quantitative two- and three-dimensional image analysis. The Core is fully equipped to support intravital microscopy, providing investigators with space in a surgical suite with all necessary supplies for surgery, anesthesia, and animal monitoring. The Microscopy Core benefits not only from the extensive facilities of the ICBM, but also the reliable performance of systems that are fastidiously maintained by ICBM staff and continuously supported with manufacturers' service contracts. Specifically, the Core will apply and adapt methods of intravital microscopy that the ICBM has developed over the past 12 years to studies of the pathophysiology of diabetes and will develop and implement novel assays of protein and cell function based upon fluorescent protein biosensors, applying a combined approach of spectral and time-resolved fluorescence quantification. The Microscopy Core is Directed by Dr. K. Dunn, with Dr. R. Day serving as Associate Director. The Core leverages the expertise of a staff scientist and technician, and interfaces closely with the Islet & Physiology Core of the IDRC to ensure that tissue resources and surgical expertise are tightly integrated. The Aims of the Microscopy Core include: (1) Implementation of methods of quantitative intravital microscopy. (2) Continued development of intravital microscopy methods that will be applied to biological systems of interest to IDRC investigators, including approaches for studies of muscle and neurons in the brain. (3) Implementation of fluorescent protein biosensors for intravital and in vitro studies, including the optimization of biosensors for confocal and multiphoton microscopy, the design of viral vectors, validation of biosensor performance, optimized methods of image collection and quantitative analysis. (4) Training in the methods of microscopy and image analysis developed by the core for investigators whose needs exceed the capacity of the core. (5) Technical support for laboratories lacking microscopy expertise to utilize additional challenging techniques; e.g., fluorescent biosensor-based in vitro studies, fluorescence lifetime microscopy).

摘要（显微镜核心） 显微镜核心的总体目标是为IDRC研究人员提供有效的先进的 光学显微镜的方法，特别是强大的活体显微镜新技术的发展 在上一个补助期。显微镜核心利用印第安纳州中心的广泛设施， 生物显微镜（ICBM），医学院的20岁的核心显微镜设施。核心是 配备了三个点扫描共聚焦/多光子激发显微镜，以及旋转盘 共聚焦显微镜和多个数字工作站支持定量二维和三维 图像分析Core配备齐全，可支持活体显微镜检查，为研究人员提供空间 在手术室里，有所有必要的手术用品，麻醉和动物监测。显微镜 核心利益不仅来自洲际弹道导弹的广泛设施，而且还来自系统的可靠性能 由洲际弹道导弹工作人员精心维护，并由制造商提供持续的服务 合同.具体来说，核心将应用和调整洲际弹道导弹所具有的活体显微镜方法。 在过去的12年中发展到糖尿病的病理生理学研究，并将制定和实施 基于荧光蛋白生物传感器的蛋白质和细胞功能的新测定， 光谱和时间分辨荧光定量的方法。显微镜核心由K博士指导。 邓恩和R博士日担任副主任。核心利用了一名科学家的专业知识， 技术人员，并与IDRC的胰岛和生理学核心密切合作，以确保组织资源 和外科专业知识紧密结合显微镜核心的目标包括： (1)活体定量显微镜检查方法的实施。 (2)继续发展活体显微镜方法，将应用于生物系统， IDRC研究人员的兴趣，包括肌肉和神经元在大脑中的研究方法。 (3)荧光蛋白生物传感器在活体和体外研究中的应用，包括 共聚焦和多光子显微镜的生物传感器的优化，病毒载体的设计， 生物传感器性能，优化的图像采集和定量分析方法。 (4)核心小组为调查人员开发的显微镜和图像分析方法培训 其需求超出了核心的容量。 (5)为缺乏显微镜专业知识的实验室提供技术支持， 技术;例如，基于荧光生物传感器的体外研究，荧光寿命显微术）。