Resource Development Core

项目摘要/摘要(资源开发核心) 资源开发核心将专注于开发技术，以帮助肾脏研究人员 认识到定量、大规模显微镜作为了解肾功能和肾功能的工具的潜力 疾病。组织清除方法为研究人员提供了对整个小鼠肾脏进行成像的能力， 提供了在其完整的背景下识别和描述整个细胞群体的机会 立体化的组织和关系。然而，有一些技术挑战阻碍了广泛的- 推广实施大规模、高分辨率显微镜作为一种有效的研究工具。《资源》 开发核心将通过以下方式应对这些挑战：(1)开发独特的多尺度/多尺度 基于一种新型混合Open-OPEN的啮齿动物和人类肾脏大尺度成像分辨率方法 TOP光片显微镜系统(2)自动图像分析前沿方法的发展 以及(3)综合成像和转录数据以进行联合分析的方法的发展。 这里开发的方法将继续纳入分子成像核心和 在出版物、在线储存库和通过IU O‘Brien显微镜工作坊传播。

Project Summary/Abstract (Resource Development Core) The Resource Development Core will focus on developing technologies to help renal investigators realize the potential of quantitative, large-scale microscopy as a tool for understanding kidney function and disease. Methods of tissue clearing provide researchers with the ability to image the entire mouse kidney, presenting the opportunity to identify and characterize the entire population of cells in the context of their full three-dimensional organization and relationship. However, there are technical challenges that hinder the wide- spread implementation of large-scale, high-resolution microscopy as an effective research tool. The Resource Development Core will address these challenges through (1) the development of a unique multi-scale/multi- resolution approach to large-scale imaging of the rodent and human kidney based upon a novel hybrid open- top lightsheet microscope system (2) the development of cutting-edge methods of automated image analysis and (3) the development of methods for integrating imaging and transcriptomic data for combined analyses. Methods developed here will be continuously incorporated into the services of the Molecular Imaging Core and disseminated in publications, online repositories and through the IU O’Brien Microscopy Workshop.