Multi-photon microscope for the Indiana Center for Biological Microscopy

描述（由申请人提供）：本申请旨在获得购买Olympus Fluoview 1000 MPE共焦/多光子荧光显微镜系统的资金。该仪器将由印第安纳州生物显微镜中心（ICBM）容纳、管理和维护，该中心是印第安纳州大学医学院和印第安纳州大学医学院的核心设施。乔治·M。奥布莱恩高级肾脏显微镜分析中心。共聚焦和多光子显微镜是该中心的基本服务，该中心拥有四台共聚焦显微镜，其中两台配备了多光子荧光显微镜。已有11年历史的BioRad MRC 1024 MP系统占当前显微镜使用的主要部分，但由于其年龄，将很快失去服务合同支持。我们试图用新的Olympus Fluoview系统替换这一过时且越来越不可靠的系统。用新的Olympus系统替换BioRad系统将：1.为用户提供一个可靠的仪器，将支持一个正在进行的制造商的服务协议，2.为用户提供具有增强性能和功能的现代化系统。所要求的系统对洲际弹道导弹来说绝对是必不可少的，因为洲际弹道导弹支持着超过68个由国家卫生研究院资助的实验室的研究，而且对本提案中所列的主要用户群体来说尤其重要，因为他们的研究已经依赖于目前的BioRad系统。除了支持印第安纳州大学的研究人员，该系统也是我们的NIH资助的奥布莱恩中心的主要显微镜，支持世界各地的肾脏研究人员的研究。这个系统的损失将严重损害洲际弹道导弹作为大学核心设施和肾脏显微镜中心的能力。获得新的奥林巴斯系统将确保在洲际弹道导弹上继续有效地使用共聚焦/多光子显微镜-拟议的仪器是许多生物医学研究人员研究疾病细胞基础的重要工具。特别是，使用该仪器进行的活体动物研究对于阐明整个生物体背景下的疾病过程和治疗具有独特的功能，使其成为临床结果的更好预测者。

DESCRIPTION (provided by applicant): This request is for funds to purchase an Olympus Fluoview 1000MPE confocal/multiphoton fluorescence microscope system. This instrument will be housed, managed and maintained by the Indiana Center for Biological Microscopy (ICBM), a core facility of the Indiana University School of Medicine and the Indiana University George M. O'Brien Center for Advanced Renal Microscopic Analysis. Confocal and multiphoton microscopy are essential services of the center, which operates four confocal microscopes, two of which are equipped for multiphoton fluorescence microscopy. An eleven-year old BioRad MRC1024MP system accounts for a major fraction of current microscope utilization, but, due to its age, will soon lose service contract support. We seek to replace this dated and increasingly unreliable system with a new Olympus Fluoview system. Replacing the BioRad system with a new Olympus system will, 1. Provide users with a reliable instrument that will be supported by an ongoing manufacturer's service agreement, 2. Provide users with a modern system with enhanced performance and capabilities. The requested system is absolutely essential to the ICBM, which supports the research of over 68 NIH- funded laboratories, and is particularly crucial to the group of major users listed in this proposal, whose research has come to depend upon the current BioRad system. In addition to supporting Indiana University researchers, this system is also the primary microscope of our NIH-funded O'Brien Center, supporting the research of renal investigators throughout the world. The loss of this system will profoundly compromise the ability of the ICBM to function as a university core facility, and as a center of renal microscopy. Acquiring the new Olympus system will ensure the continued productive use of confocal/multiphoton microscopy at the ICBM Relevance - The proposed instrument is a crucial tool in the research of numerous biomedical investigators studying the cellular basis of disease. In particular, studies of living animals conducted with this instrument are uniquely powerful for elucidating disease processes and therapies in the context of the whole organism, making them better predictors of clinical outcomes.