Multi-photon microscope for the Indiana Center for Biological Microscopy

描述（由申请人提供）：本申请用于购买奥林巴斯Fluoview 1000MPE共聚焦/多光子荧光显微镜系统。该仪器将由印第安纳大学医学院和印第安纳大学乔治·m·奥布莱恩高级肾脏显微分析中心的核心设施印第安纳生物显微镜中心（ICBM）进行安置、管理和维护。共聚焦和多光子显微镜是中心的基本服务，该中心有四个共聚焦显微镜，其中两个配备了多光子荧光显微镜。已有11年历史的BioRad MRC1024MP系统占当前显微镜利用率的主要部分，但由于其年龄，将很快失去服务合同支持。我们寻求用新的奥林巴斯Fluoview系统取代这个过时且越来越不可靠的系统。用新的奥林巴斯系统替换BioRad系统将：1 .为用户提供可靠的仪器，该仪器将由持续的制造商服务协议支持；为用户提供具有增强性能和功能的现代系统。所要求的系统对于洲际弹道导弹是绝对必要的，它支持超过68个NIH资助的实验室的研究，对于本提案中列出的主要用户群体尤其重要，他们的研究已经依赖于当前的BioRad系统。除了支持印第安纳大学的研究人员外，该系统也是美国国立卫生研究院资助的奥布莱恩中心的主要显微镜，支持世界各地肾脏研究人员的研究。该系统的丧失将严重影响洲际弹道导弹作为大学核心设施和肾脏显微镜中心的功能。获得新的奥林巴斯系统将确保在ICBM相关性中继续有效地使用共聚焦/多光子显微镜-该仪器是众多生物医学研究人员研究疾病细胞基础的重要工具。特别是，用该仪器进行的活体动物研究对于阐明整个生物体的疾病过程和治疗具有独特的强大作用，使其更好地预测临床结果。

DESCRIPTION (provided by applicant): This request is for funds to purchase an Olympus Fluoview 1000MPE confocal/multiphoton fluorescence microscope system. This instrument will be housed, managed and maintained by the Indiana Center for Biological Microscopy (ICBM), a core facility of the Indiana University School of Medicine and the Indiana University George M. O'Brien Center for Advanced Renal Microscopic Analysis. Confocal and multiphoton microscopy are essential services of the center, which operates four confocal microscopes, two of which are equipped for multiphoton fluorescence microscopy. An eleven-year old BioRad MRC1024MP system accounts for a major fraction of current microscope utilization, but, due to its age, will soon lose service contract support. We seek to replace this dated and increasingly unreliable system with a new Olympus Fluoview system. Replacing the BioRad system with a new Olympus system will, 1. Provide users with a reliable instrument that will be supported by an ongoing manufacturer's service agreement, 2. Provide users with a modern system with enhanced performance and capabilities. The requested system is absolutely essential to the ICBM, which supports the research of over 68 NIH- funded laboratories, and is particularly crucial to the group of major users listed in this proposal, whose research has come to depend upon the current BioRad system. In addition to supporting Indiana University researchers, this system is also the primary microscope of our NIH-funded O'Brien Center, supporting the research of renal investigators throughout the world. The loss of this system will profoundly compromise the ability of the ICBM to function as a university core facility, and as a center of renal microscopy. Acquiring the new Olympus system will ensure the continued productive use of confocal/multiphoton microscopy at the ICBM Relevance - The proposed instrument is a crucial tool in the research of numerous biomedical investigators studying the cellular basis of disease. In particular, studies of living animals conducted with this instrument are uniquely powerful for elucidating disease processes and therapies in the context of the whole organism, making them better predictors of clinical outcomes.