Multi-photon microscope for the Indiana Center for Biological Microscopy

描述(申请人提供)：此申请是为购买奥林巴斯FluoView 1000MPE共焦/多光子荧光显微镜系统而提出的资金申请。这台仪器将由印第安纳大学医学院的核心机构印第安纳生物显微镜中心(ICBM)和印第安纳大学乔治·M·奥布莱恩高级肾脏显微分析中心负责管理和维护。共焦显微镜和多光子显微镜是该中心的基本服务，该中心运营着四台共焦显微镜，其中两台配备了多光子荧光显微镜。已有11年历史的BioRad MRC1024MP系统占当前显微镜使用量的一大部分，但由于其年代久远，很快将失去服务合同支持。我们寻求用新的奥林巴斯FluoView系统取代这个过时且越来越不可靠的系统。用新的奥林巴斯系统替换BioRad系统将：1.为用户提供可靠的仪器，该仪器将得到正在进行的制造商服务协议的支持；2.为用户提供性能和功能增强的现代系统。所要求的系统对ICBM是绝对必要的，ICBM支持68个以上由NIH资助的实验室的研究，对本提案中列出的主要用户群体尤其关键，他们的研究已经依赖于当前的BioRad系统。除了支持印第安纳大学的研究人员，该系统也是我们由美国国立卫生研究院资助的奥布莱恩中心的主要显微镜，支持世界各地肾脏研究人员的研究。这一系统的丧失将严重损害ICBM作为大学核心设施和肾脏显微镜中心的能力。收购新的奥林巴斯系统将确保在与洲际弹道导弹相关的情况下继续有效地使用共焦/多光子显微镜--拟议中的仪器是众多生物医学研究人员研究疾病细胞基础的关键工具。特别是，使用该仪器对活体动物进行的研究在阐明整个有机体范围内的疾病过程和治疗方法方面具有独特的强大功能，使它们能够更好地预测临床结果。

DESCRIPTION (provided by applicant): This request is for funds to purchase an Olympus Fluoview 1000MPE confocal/multiphoton fluorescence microscope system. This instrument will be housed, managed and maintained by the Indiana Center for Biological Microscopy (ICBM), a core facility of the Indiana University School of Medicine and the Indiana University George M. O'Brien Center for Advanced Renal Microscopic Analysis. Confocal and multiphoton microscopy are essential services of the center, which operates four confocal microscopes, two of which are equipped for multiphoton fluorescence microscopy. An eleven-year old BioRad MRC1024MP system accounts for a major fraction of current microscope utilization, but, due to its age, will soon lose service contract support. We seek to replace this dated and increasingly unreliable system with a new Olympus Fluoview system. Replacing the BioRad system with a new Olympus system will, 1. Provide users with a reliable instrument that will be supported by an ongoing manufacturer's service agreement, 2. Provide users with a modern system with enhanced performance and capabilities. The requested system is absolutely essential to the ICBM, which supports the research of over 68 NIH- funded laboratories, and is particularly crucial to the group of major users listed in this proposal, whose research has come to depend upon the current BioRad system. In addition to supporting Indiana University researchers, this system is also the primary microscope of our NIH-funded O'Brien Center, supporting the research of renal investigators throughout the world. The loss of this system will profoundly compromise the ability of the ICBM to function as a university core facility, and as a center of renal microscopy. Acquiring the new Olympus system will ensure the continued productive use of confocal/multiphoton microscopy at the ICBM Relevance - The proposed instrument is a crucial tool in the research of numerous biomedical investigators studying the cellular basis of disease. In particular, studies of living animals conducted with this instrument are uniquely powerful for elucidating disease processes and therapies in the context of the whole organism, making them better predictors of clinical outcomes.