Trace Elements (TE)

微量元素 (TE)

• 批准号: 10165517
• 负责人: Brian P Jackson
• 金额: $ 2.11万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10165517
• 关键词: Animal Organ; Arsenic; Beverages; Biological; Blood; Budgets; Cancer Center; Cancer Center Support Grant; Carcinogens; Catchment Area; Cells; Centers for Disease Control and Prevention (U.S.); Cereals; Chemicals; Chromatography; Coupled; Data; Data Analytics; Elements; Ensure; Exposure to; Fishes; Funding; Hair; Health Policy; Human; Individual; Inductively Coupled Plasma Mass Spectrometry; Infant; Inorganic Chemistry; Institution; International; Invertebrates; Iron; Link; Malignant Neoplasms; Measures; Methods; Monitor; NCI Center for Cancer Research; Norris Cotton Cancer Center; Nutrient; Placenta; Platinum; Play; Population Sciences; Preparation; Protocols documentation; Public Health; Quality Control; Research; Research Personnel; Resource Sharing; Resources; Role; Running; Sampling; Selenium; Services; Soil; Structure of nail of toe; Technology; Testing; Therapeutic Effect; Therapeutic Uses; Tissues; Trace Elements; Urine; Water; Work; analytical method; animal tissue; base; bioimaging; case-by-case basis; early life exposure; granite; instrument; instrumentation; member; nanoparticle; programs; quality assurance

Project Summary/Abstract: Trace Elements The chemistry of inorganic elements is linked inextricably with the study of cancer. This is especially true for our catchment area, given the high level of inorganic element exposures typical of “the Granite State”. Whether studying known carcinogens, such as arsenic, the potential antagonistic effects of elements, such as selenium, or the therapeutic uses of platinum or iron nanoparticles, there is a clear need for both routine and advanced trace element analytical methods to support NCCC research. The Trace Elements (TE) Shared Resource provides access to low-level inorganic elemental analysis to all four research programs within the Cancer Center. TE uses state-of-the-art ICP-MS instruments to quantify multiple elements and, when coupled with chromatography, multiple contaminant compounds (species) in a variety of biological and environmental media. Cutting-edge ICP-MS instrumentation (e.g., triple-quadrupole ICP-QQQ) provides rapid, interference- free analysis of established and emerging contaminants and macro- and micro-nutrient elements. The breadth of sample types handled by TE spans water, soil, sediment, invertebrates, fish and animal tissues and organs, cell lysates, human hair, toenail, blood, urine, placenta, foodstuffs, and beverages. The ability to handle such a broad array of sample types is a unique feature of TE. In some cases, the sample mass available for analysis is <10 mg, and methods often are adapted to account for small sample masses on a case-by-case basis. Analytical data must be collected according to accepted protocols for sample preparation, analysis, and quality control. TE has established protocols and a Quality Assurance program, uses methods based on those of the CDC, FDA, EPA, and USGS; and participates in national and international proficiency testing programs to ensure accurate, precise, and defensible data. TE has very strong links to the Cancer Population Sciences (CPS) Program. TE is an internationally-recognized center for low-level analysis of chemical elements and their compounds in biological tissues. TE has provided services for 27 other US academic institutions over the past funding cycle and fulfills a unique niche in providing state-of-the-art analytical services in trace elemental analysis. Over the last funding cycle, TE has run 6,721 samples for 12 NCCC Members from all 4 NCCC Programs (CPS [1], CBT [1], ICI [2], and TEC [8]). Funded NCCC Members have represented 15% (10) of Total Users, and we are requesting only 3% of the Total TE Budget from CCSG support. One focus of the CPS Program is early life exposure to arsenic, and TE is integral to the determination and speciation of arsenic for this project. This work has directly informed state and national public health policy. For example, the US FDA announced a proposed limit for inorganic arsenic in infant cereal of 100 ppb in 2016. For the TEC Program, TE provided a method for measuring iron nanoparticles in animal tissue. TE has contributed to CBT investigators monitoring the therapeutic effects of platinum. TE is demonstrably an essential, efficient and high-impact resource that continues to play a pivotal role in NCCC research.

项目概要/摘要：微量元素 无机元素的化学与癌症的研究密不可分。更是如此 鉴于“花岗岩州”典型的无机元素暴露水平高，我们的集水区。是否 研究已知的致癌物，如砷，元素的潜在拮抗作用，如硒， 或铂或铁纳米颗粒的治疗用途，显然需要常规和先进的 支持NCCC研究的微量元素分析方法。微量元素（TE）共享资源 为癌症研究中心的所有四个研究项目提供低水平无机元素分析 中心TE使用最先进的ICP-MS仪器对多种元素进行定量， 色谱，多种生物和环境中的多种污染物化合物（物种） 媒体尖端的ICP-MS仪器（例如，三重四极ICP-QQQ）提供快速、干扰- 免费分析现有和新出现的污染物以及大量和微量营养元素。广度 TE处理的样品类型涵盖水、土壤、沉积物、无脊椎动物、鱼类和动物组织和器官， 细胞裂解物、人头发、脚趾甲、血液、尿液、胎盘、食品和饮料。处理这样一个 广泛的样品类型是TE的独特之处。在某些情况下，可用于分析的样品质量 <10 mg，并且方法通常根据具体情况进行调整，以说明小样本质量。 分析数据必须根据公认的样品制备、分析和质量协议收集 控制TE已制定了协议和质量保证计划，并使用基于 CDC、FDA、EPA和USGS;并参与国家和国际能力验证计划， 确保数据的准确性、精确性和可靠性。TE与癌症人口科学有着密切的联系 (CPS)程序. TE是国际公认的化学元素低水平分析中心， 它们的化合物在生物组织中。TE已为27个其他美国学术机构提供服务， 过去的资金周期，并在提供最先进的痕量元素分析服务方面实现了独特的利基 分析.在上一个资助周期中，TE为所有4个NCCC的12个NCCC成员运行了6，721个样本 程序（CPS [1]、CBT [1]、ICI [2]和TEC [8]）。受资助的NCCC成员占15%（10） 总用户，我们只要求CCSG支持总TE预算的3%。CPS的一个重点 项目是生命早期砷暴露，TE是砷的测定和形态分析的组成部分， 这个项目这项工作直接为州和国家公共卫生政策提供了信息。例如，美国FDA 在2016年宣布了婴儿谷物中无机砷的建议限量为100 ppb。对于TEC计划，TE 提供了一种用于测量动物组织中的铁纳米颗粒的方法。TE为CBT调查人员做出了贡献 监测铂的治疗效果事实证明，TE是一个必不可少的、高效的和高影响力的 资源，继续发挥在NCCC研究的关键作用。