Biomedical National Elemental Imaging Resource (BNEIR)

生物医学国家元素成像资源 (BNEIR)

• 批准号: 10614502
• 负责人: Brian P Jackson
• 金额: $ 46.42万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10614502
• 关键词: Ablation; Acceleration; Aluminum; Alzheimer' s Disease; Antibodies; Area; Arsenicals; Basic Science; Biochemical; Biological Markers; Biological Process; Biology; Brain; Cell Cycle Regulation; Cell physiology; Cells; Centers of Research Excellence; Chemicals; Coenzymes; Collaborations; Communities; Computer software; Consult; Contrast Media; Copper; Custom; Cytometry; Data Analyses; Detection; Diagnosis; Disease; Doctor of Philosophy; Drug Delivery Systems; Drug Targeting; E-learning; Elements; Ensure; Fees; Fluorescence; Fostering; Foundations; Funding; Gadolinium; Goals; Health; Hepatolenticular Degeneration; Homeostasis; Human; Human Resources; Human body; Image; Imaging Techniques; Immunity; Immunohistochemistry; Inductively Coupled Plasma Mass Spectrometry; Infrastructure; Institution; International; Internet; Iron; Isotopes; Knowledge; Laboratories; Lasers; Life; Lifting; Light; Link; Location; Magnetic Resonance Imaging; Metals; Mission; Molecular; National Institute of General Medical Sciences; Online Systems; Organ; Organism; Performance; Persons; Platinum Compounds; Prevention; Process; Proteins; Reproduction; Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Roentgen Rays; Role; Science; Scientist; Selenium; Senile Plaques; Services; Source; Spatial Distribution; Spectrometry, Mass, Secondary Ion; Spottings; Synchrotrons; System; Techniques; Technology; Therapeutic; Time; Tissues; Trace Elements; Training; Training Programs; United States National Institutes of Health; Vacuum; Visit; Work; Zinc; base; beamline; bioimaging; biological systems; biomedical facility; biomedical resource; cancer therapy; cofactor; college; data acquisition; data handling; detection limit; diagnostic tool; disease diagnosis; drug efficacy; experience; experimental study; human disease; improved; industry partner; innovation; instrumentation; mass spectrometer; medical schools; meetings; metalloenzyme; nanoparticle; programs; repaired; response; software development; success; synchrotron radiation; webinar

Up to 60 chemical elements can be detected in the human body, about 25 of which are essential to life. Elements such as iron (Fe), zinc (Zn), copper (Cu), and selenium (Se) are constituents of enzyme cofactors required for a wide range of processes from cell cycle regulation, reproduction, structural repair, and immunity. Dysregulation of these elements is a hallmark of disease. For instance, aluminum (Al), Fe, Cu and Zn accumulation in amyloid plaques in the brain are associated with Alzheimer’s disease and dysregulation of Cu causes Menkes and Wilson’s disease. Chemical elements are also diagnostic tools and chemotherapeutics; gadolinium (Gd) is the most common magnetic resonance imaging contrast agent, metal-base nanoparticles show promise for targeted drug delivery and platinum (Pt) and arsenic (As) compounds are used in cancer treatment. Invariably, knowing the spatial distribution of an element in an organ, tissue or cell is essential to fully investigate mechanisms of disease or the efficacy of drug delivery. Hence, elemental imaging at the micron scale is essential to further the NIGMS goal to support basic research that increases our understanding of biological processes and lays the foundation for advances in disease diagnosis, treatment, and prevention. Current analytical resources for biomedical elemental imaging are either over-subscribed (synchrotron X-ray fluorescence), cover only a limited range of elements, are performed under a vacuum or not readily scalable to imaging at the mm scale. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a laboratory-based elemental imaging (elemental imaging) technique that it is increasingly being applied to biomedical applications with comparable or lower detection limits than other elemental imaging techniques and, potentially, easier access and operability. To date there are no elemental imaging LA-ICP-MS user resources in the US. The Dartmouth Trace Element Analysis Core is an established and highly regarded shared resource that supports researchers throughout the US, is staffed by experienced researchers in elemental imaging and ICP-MS, uses state-of-the-art instrumentation and has excellent links to industry partners. Our aims are to expand upon our current ad hoc elemental imaging service to establish an elemental imaging user facility; the Biomedical National Elemental Imaging Resource (BNEIR), which will accelerate and simplify access for biomedical researchers to instrumentation, expertise, web-based and in-person training, after-visit support, software and will foster a dynamic community for elemental imaging users. We will actively promote the elemental imaging within the larger NIGMS community of scientists through webinars, seminars, attendance at national and international meetings and actively seeking out NIH researchers currently pursuing relevant research projects.

在人体内可以检测到多达60种化学元素，其中约25种是生命所必需的。铁(Fe)、锌(Zn)、铜(Cu)和硒(Se)等元素是从细胞周期调节、生殖、结构修复和免疫等广泛过程中所需的酶辅助因子的组成部分。这些元素的失调是疾病的一个标志。例如，铝(Al)、铁、铜和锌在大脑淀粉样斑块中的积累与阿尔茨海默病有关，而铜的调节失调会导致门克斯和威尔逊病。化学元素也是诊断工具和化疗药物；格拉德(Gd)是最常见的磁共振成像造影剂，金属基纳米颗粒显示出靶向药物输送的前景，铂(铂)和砷(As)化合物被用于癌症治疗。一如既往，了解器官、组织或细胞中元素的空间分布对于全面研究疾病机制或药物输送的有效性是至关重要的。因此，微米级的元素成像对于推进NIGMS的目标至关重要，NIGMS的目标是支持基础研究，以增加我们对生物过程的理解，并为疾病诊断、治疗和预防方面的进步奠定基础。目前用于生物医学元素成像的分析资源要么供过于求(同步加速器X射线荧光)，只覆盖有限范围的元素，要么在真空中进行，要么难以扩展到毫米级的成像。激光烧蚀电感耦合等离子体质谱(LA-ICPMS)是一种以实验室为基础的元素成像(元素成像)技术，它正越来越多地被应用于生物医学应用，其检测下限与其他元素成像技术相当或更低，并且潜在地更容易获得和操作。到目前为止，美国还没有基本的成像LA-ICP-MS用户资源。达特茅斯痕量元素分析核心是一个成熟和备受推崇的共享资源，支持全美的研究人员，拥有元素成像和电感耦合等离子体质谱领域经验丰富的研究人员，使用最先进的仪器，并与行业合作伙伴建立了良好的联系。我们的目标是扩展我们目前的特别基本成像服务，以建立一个基本成像用户设施；生物医学国家基本成像资源(BNEIR)，它将加速和简化生物医学研究人员获取仪器、专业知识、基于网络的和面对面培训、参观后支持和软件的途径，并将为基本成像用户培养一个充满活力的社区。我们将通过网络研讨会、研讨会、出席国内和国际会议以及积极寻找目前正在从事相关研究项目的NIH研究人员，在更大的NIGMS科学家社区内积极促进元素成像。