Molecular anatomy resources for postembryonic zebrafish

项目总结 斑马fish是一种应用广泛的生物医学模式生物，在早期得到了广泛的研究。 目前的研究工作越来越多地集中在与理解基础AS相关的后期阶段。 对脊椎动物发育以及人类疾病、出生缺陷和再生潜力的展望。这 该项目将提供一个资源，通过识别特定fic细胞类型的分子标记来促进这类研究。 出现在后期，并绘制这些标记及其在发育中的解剖学中所代表的细胞 幼虫fish.候选标记和细胞类型将使用无偏向单细胞转录进行鉴定 入路和解剖测绘将使用组织学和成像方法完成，所有 这是这个实验室的常规做法。这些资源将使我们调查界的努力- Ing Zebrafish用于生物医学研究，并将为进一步开发该系统用于以下领域提供机会- 坚持和促进人类健康。

PROJECT SUMMARY The zebrafish is a widely used biomedical model organism and has been studied extensively at early stages. Current research efforts focus increasingly on later stages that are relevant to understanding fundamental as- pects of vertebrate development as well as human disease, birth defects and regenerative potential. This project will provide a resource to facilitate such studies by identifying molecular markers for specific cell types present at late stages, and mapping these markers and the cells they represent within the developing anatomy of the larval fish. Candidate markers and cell types will be identified using unbiased single cell transcriptomic approaches, and anatomical mapping will be accomplished using histological and imaging approaches, all of which are routine for this laboratory. These resources will enable efforts by the community of investigators us- ing zebrafish for biomedical research, and will provide opportunities to further exploit this system for under- standing and promoting human health.