EQUIPMENT SUPPLEMENT REQUEST: Cellular Interactions Underlying Establishment and Implementation of Zebrafish Adult Pigment Pattern

设备补充请求：斑马鱼成体色素模式建立和实施的细胞相互作用

• 批准号: 9273884
• 负责人: DAVID M PARICHY
• 金额: $ 13.25万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9273884
• 关键词: Address; Adult; Affect; Behavior; Binding; Biological; Cell Transplantation; Cells; Cellular biology; Communication; Complex; Congenital Abnormality; Defect; Development; Disease; Drosophila genus; Embryo; Event; Genes; Genetic; Genital system; Goals; Growth; Health; Homeostasis; Human; Invaded; Knowledge; Light; Location; Logic; Maintenance; Malignant Neoplasms; Melanophores; Molecular; Morphogenesis; Natural regeneration; Neoplasm Metastasis; Neural Crest; Neural Crest Cell; New Territories; Oranges; Organogenesis; Outcome; Pattern; Pattern Formation; Phenotype; Pigments; Population; Proteins; Public Health; Research; Role; Semaphorins; Signal Pathway; Signal Transduction; Specific qualifier value; System; Testing; Time; Tissues; Transgenic Organisms; Translating; Variant; Wound Healing; Zebrafish; Zinc; basonuclin; cell behavior; cell motility; cell type; embryo tissue; innovation; insight; malformation; melanocyte; melanoma; mutant; notch protein; progenitor; prospective; response; tissue regeneration

 DESCRIPTION (provided by applicant): The development of boundaries between cells and tissues is a critical feature of pattern formation and these same boundaries must be maintained during later adult homeostasis. The mechanisms of boundary establishment and implementation are thus of fundamental biological significance and have profound implications for understanding birth defects as well as cancers, in which boundary constraints are lost, and regeneration, in which boundaries must be formed anew. The goal of this project is to understand the genetic and cellular mechanisms underlying an especially tractable example of boundaries in a post-embryonic vertebrate, the adult pigment stripes of zebrafish. Previous efforts demonstrated that interactions between different classes of pigment cells are essential for stripe development and maintenance, though the particular genes and cell behaviors involved have been elusive. In Aim 1, innovative hypotheses to explain the specification of stripes and their dynamic reiteration will be tested using mutational and transgenic approaches, cell transplantation and analyses of a naturally occurring pattern variant. In Aim 2, efforts will focus on mechanisms of subsequent pat- tern implementation, testing roles for specific cellular behaviors and signaling pathways in refining a striped pattern once it has been established, and how these factors have changed to generate a pattern that lacks stripes. These studies will provide valuable new insights into pigment cell boundary formation, with relevance to human health and development, and promise to shed light on the logic of boundary forming mechanisms more generally.

 描述（由申请人提供）：细胞和组织之间边界的发展是模式形成的关键特征，这些相同的边界必须在成年后的体内平衡中保持。因此，边界建立和实施的机制具有基本的生物学意义，并对理解出生缺陷和癌症具有深远的影响，在这些疾病中，边界约束丢失，再生，必须重新形成边界。这个项目的目标是了解遗传和细胞机制的基础上，特别是在胚胎后脊椎动物，斑马鱼的成年色素条纹边界的例子。以前的研究表明，不同种类的色素细胞之间的相互作用对条纹的发育和维持至关重要，尽管所涉及的特定基因和细胞行为一直难以捉摸。在目标1中，将使用突变和转基因方法、细胞移植和分析自然发生的模式变体来测试解释条纹规格及其动态重复的创新假设。在目标2中，工作将集中在后续模式实施的机制上，测试特定细胞行为和信号通路在条纹模式建立后的细化中的作用，以及这些因素如何改变以产生缺乏条纹的模式。这些研究将为色素细胞边界形成提供有价值的新见解，与人类健康和发育相关，并有望更普遍地阐明边界形成机制的逻辑。