Developmental origins and homeostatic mechanisms underlying adult phenotypes: multispectral sorting of pigment cells from zebrafish and non-traditional model species

成体表型的发育起源和稳态机制：斑马鱼和非传统模型物种色素细胞的多光谱分选

• 批准号: 10799015
• 负责人: DAVID M PARICHY
• 金额: $ 25万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10799015
• 关键词: Adult; Award; Biological Models; Breeding; Cell Communication; Cells; Congenital Abnormality; Dedications; Development; Distant; Equipment; Fishes; Genetic Diseases; Goals; Homeostasis; In Vitro; Laboratories; Modeling; Parents; Pattern; Pattern Formation; Phenotype; Pigments; Principal Investigator; Public Health; Regenerative Medicine; Research; Sorting; Testing; Tissues; Work; Zebrafish; cell type; fluorescence activated cell sorter device; genetic resource; genome resource; innovation; instrument; marine; mutant; novel strategies; parent project; repaired; self organization; single-cell RNA sequencing

Contact PD/PI: PARICHY, DAVID M SUMMARY OF SUPPLEMENT REQUEST The parent project focuses on elucidating mechanisms of pigment cell type and pigment pattern diversification in the fishes of the genus Danio, which includes the zebrafish D. rerio, as well as more distantly related species. This equipment supplement request seeks purchase of a Cytek Aurora CS fluorescence activated cell sorter that will greatly enhance our ability to meet the original goals of the award, which require extensive sin- gle cell RNA-sequencing analyses of pigment cells from wild-type and mutant backgrounds of zebrafish and other danios, an approach that is proving to be technically intractable and financially infeasible with the instru- ment currently available. The Cytek Aurora CS will also permit us to take an innovative, in vitro approach to elucidating mechanisms of cell-cell interactions during pattern formation in zebrafish, while uniquely allowing us to test new hypotheses pertaining to evolutionary diversification of cell types using marine fishes (e.g., the wrasse Cirrhilabrus finfenmaa) and other species (e.g., rasbora Trigonostigma heteromorpha) that cannot be bred in the laboratory and that lack the genetic and genomic resources of zebrafish. 1 Project Summary/Abstract Page 6

联系PD/PI：PARICHY，大卫M 申请摘要 母项目的重点是阐明色素细胞类型和色素模式多样化的机制 在Danio属的鱼类中，包括斑马鱼D。rerio，以及更远的亲戚 物种本设备补充申请寻求购买Cytek Aurora CS荧光激活细胞 分拣机，这将大大提高我们的能力，以满足奖励的原始目标，这需要广泛的罪恶- 斑马鱼野生型和突变型背景色素细胞的gle细胞RNA测序分析， 其他danios，一种被证明在技术上难以处理和财政上不可行的方法， 目前可用。Cytek Aurora CS还将使我们能够采用创新的体外方法， 阐明了斑马鱼模式形成过程中细胞间相互作用的机制，同时独特地允许 我们使用海洋鱼类测试有关细胞类型进化多样性的新假设（例如，的 丝隆头鱼（Cirrhilabrus finfenmaa）和其它物种（例如，三角柱鱼），不能 在实验室中培育，缺乏斑马鱼的遗传和基因组资源。 1 项目摘要/摘要第6页