Molecular anatomy resources for postembryonic zebrafish

胚胎后斑马鱼的分子解剖资源

• 批准号: 10402832
• 负责人: DAVID M PARICHY
• 金额: $ 8.08万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-10 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10402832
• 关键词: Adult; Anatomy; Animal Model; Atlases; Biomedical Research; Cells; Communities; Congenital Abnormality; Data; Databases; Development; Disease; Embryo; Event; Fishes; Health; Human; In Situ Hybridization; Investigation; Laboratories; Larva; Location; Messenger RNA; Molecular; Morphogenesis; Natural regeneration; Organ; Organogenesis; Pattern; Phenotype; Process; Research; Research Personnel; Resources; Spatial Distribution; System; Tissues; Work; Zebrafish; candidate marker; cell type; diagnostic biomarker; histological image; human disease; imaging approach; molecular marker; mutant; regeneration potential; single-cell RNA sequencing; transcriptomics; zebrafish development

PROJECT SUMMARY The zebrafish is a widely used biomedical model organism and has been studied extensively at early stages. Current research efforts focus increasingly on later stages that are relevant to understanding fundamental as- pects of vertebrate development as well as human disease, birth defects and regenerative potential. This project will provide a resource to facilitate such studies by identifying molecular markers for specific cell types present at late stages, and mapping these markers and the cells they represent within the developing anatomy of the larval fish. Candidate markers and cell types will be identified using unbiased single cell transcriptomic approaches, and anatomical mapping will be accomplished using histological and imaging approaches, all of which are routine for this laboratory. These resources will enable efforts by the community of investigators us- ing zebrafish for biomedical research, and will provide opportunities to further exploit this system for under- standing and promoting human health.

项目摘要 斑马鱼是一种广泛使用的生物医学模式生物，并在早期阶段进行了广泛的研究。 目前的研究工作越来越多地集中在与理解基础知识相关的后期阶段， 脊椎动物发育以及人类疾病、出生缺陷和再生潜力的前景。这 该项目将通过识别特定细胞类型的分子标记物，提供促进此类研究的资源 并绘制这些标记物和它们在发育中的解剖结构中所代表的细胞 鱼的幼虫。候选标志物和细胞类型将使用无偏单细胞转录组学方法鉴定艾德。 方法和解剖标测将使用组织学和成像方法完成，所有 这是本实验室的常规程序这些资源将使我们的调查人员能够作出努力- 将斑马鱼用于生物医学研究，并将为进一步利用这一系统提供机会， 维护和促进人类健康。