DIVERSITY SUPPLEMENT TO DEVELOPMENTAL ORIGINS AND HOMEOSTATIC MECHANISMS UNDERLYING ADULT PHENOTYPES

项目总结

PROJECT SUMMARY Parent award. Mechanisms underlying the origin and maintenance of adult form, and naturally occurring varia- tion in adult form, remain poorly understood. This research program seeks to elucidate how gene activities are translated through cellular behaviors into specific morphological outcomes at adult stages. Such information will contribute to understanding patterning and morphogenetic mechanisms essential for postembryonic devel- opment, with relevance to human genetic disease, birth defects, aging and regenerative medicine. For these efforts, the work uses pigmentation of zebrafish, its close relatives in the genus Danio, and more distantly re- lated teleost fishes. Pigment cells in these animals and other vertebrates arise from embryonic neural crest cells that also contribute to a wide variety of other tissues and organs, including most of the peripheral nervous system and craniofacial skeleton. Defects in neural crest derived lineages generally, and pigment cells specifi- cally, are associated with numerous hereditary pathologies as well as cancers, including melanoma. During normal development, pigment cells that arise either directly from neural crest cells or indirectly through postembryonic stem cell intermediates organize into highly stereotyped, largely two dimensional patterns in the transparent skin. Cell behaviors during pattern formation are readily observed as phenotypes develop, and ge- netic mechanisms are accessible through mutational analyses and other approaches, both in striped zebrafish and in other species having very different adult patterns. The work described here builds on prior effort in this program, and takes an unusually integrative approach to understand pattern and pattern variation, combining manipulative experiments, genetic analysis, high resolution imaging, cutting edge genomics, comparative biol- ogy and behavioral assays. Goals in the coming years are to elucidate: (i) mechanisms by which pigment cell progenitors are specified for different pigment cell types during development, and how diversification of cell types has been achieved evolutionarily; (ii) genetic and cellular mechanisms underlying self-organizing interac- tions among pigment cells that are essential for pattern formation, and how these interactions and permissive factors have changed to generate alternative pattern states among species; (iii) the roles of positional informa- tion in the tissue environment in setting the location of discrete pattern elements that are essential for estab- lishing pattern, and how such information contributes to qualitatively different types of pattern across species. These efforts will provide novel insights into pattern development and cell type diversification over both devel- opmental and evolutionary time. General principles uncovered will likely be applicable to a wide range of traits that depend to varying degrees cell type diversification, self-organizing cellular interactions, and positional in- formation derived from tissue environments. Supplement request. Pilot research is designed to begin answering some basic questions pertaining to thyroid hormone activities during adult pigment pattern formation, with the goal of uncovering mechanisms of TH ef- fects at cellular and transcriptomic levels, while providing a graduate trainee new skills and professional devel- opment towards initiating his dissertation research and pursuing an NRSA F31 individual fellowship. 1