Histology

组织学核心：项目总结/摘要 科赫研究所的成员使用脊椎动物模式生物作为研究已知或假定的 癌症基因的发展和致瘤性;研究肿瘤发生，发展的机制 和转移;评估基质和免疫反应在肿瘤发生中的作用;并评估疗效。 药物、纳米材料和设备在治疗和诊断方面的应用。因此，中心必须 成员能够正确诊断发育和肿瘤表型，评估潜在的 分子事件，并准确和定量地评估药物或疫苗输送，局部组织反应， 和总体治疗反应。科赫研究所组织学核心是一个共享资源，提供国家- 最先进的组织学服务来支持这些研究。这包括组织方面的援助和/或培训， 切片、玻片制备、染色和分析，以及获得 国际公认的兽医病理学家Roderick Bronson博士，负责肿瘤和组织的诊断 表型。 在本报告所述期间，组织学核心的使用率从67%上升到77%， 包括来自所有三个项目的研究人员。为了满足中心成员的需求，组织学核心 扩展服务和仪器。这包括扩大免疫组织化学和 特殊染色和独立切片的研究者培训。核心还获得了新的数字幻灯片 扫描能力和量化软件，并对现有的关键仪器进行了升级。因此，在本发明中， 这种共享资源对于科赫研究所使命的成功至关重要。 在即将到来的时期，组织学核心致力于提供广泛的最先进的组织学 支持中心成员的研究计划。计划采取的举措包括： 整合基因组学和生物信息学核心，建立在基因表达图谱的方法， 组织内的单细胞水平;开发专业知识并提供使用自动AI驱动的指导 用于组织学切片分析的分析包（如QuPath）;评估仪器， 确定荧光数字载玻片扫描的社区效益;扩大组织学培训， 包括更多实际操作讲习班机会。这一共享核心对CCSG具有特殊价值，因为 科赫研究所成员占核心服务使用量的95%。值得注意的是， 第49年比本期（第48年）预算少6.8%。

Histology Core: Project Summary/Abstract Koch Institute Members use vertebrate model organisms as a key tool to study the role of known or putative cancer genes in development and tumorigenicity; investigate the mechanisms of tumor initiation, progression and metastasis; evaluate the role of stroma and immune responses in tumorigenesis; and assess the efficacy of drugs, nanomaterials and devices in therapeutic and diagnostic applications. Thus, it is essential that Center Members are able to correctly diagnose developmental and tumor phenotypes, evaluate the underlying molecular events, and accurately and quantitatively assess drug or vaccine delivery, localized tissue response, and overall therapeutic response. The Koch Institute Histology Core is a Shared Resource that provides state- of-the-art histological services to support these studies. This includes assistance and/or training in tissue sectioning, slide preparation, staining and analysis, and access to the consultative services of an internationally recognized Veterinary Pathologist, Dr. Roderick Bronson, for diagnosis of tumor and tissue phenotypes. During the current period, usage of the Histology Core increased from 67% to 77% of Center Members, and included investigators from all three Programs. In response to Center member needs, the Histology Core expanded services and instrumentation. This includes expanded offerings for immunohistochemistry and special stains, and investigator training for independent sectioning. The Core also acquired new digital slide scanning capabilities and quantification software, and made upgrades to existing key instrumentation. Thus, this Shared Resource is essential to the success of the Koch Institute mission. In the upcoming period, The Histology Core is committed to offering a wide range of state-of-the-art histological services to support the research programs of Center Members. Planned initiatives include: working with the Integrated Genomics & Bioinformatics Core to establish methodologies for mapping gene expression at the single cell level within tissues; developing expertise and providing guidance in use of automated AI-driven analysis packages (such as QuPath) for analysis of histological sections; evaluating instrumentation to determine community benefit for fluorescent digital slide scanning; and expanding histology training offerings to include more hands-on workshop opportunities. This shared Core is of exceptional value to the CCSG because Koch Institute Members account for 95% of the Core services usage. Notably, the requested CCSG budget for Year 49 is 6.8% less than the budget in the current period (Year 48).