Microbiology, Track 4: Whole Genome Sequencing

项目摘要 微生物学，轨道4：全基因组测序 NMSU食品安全实验室(FSL)将努力实现蕨类目标，确保 训练有素的工作人员将参与蕨类活动，包括对细菌分离株进行测序，参与 熟练程度测试，并按要求执行其他分析。 NMSU FSL建议对400种或更多病原体(单核细胞增多性李斯特菌， 沙门氏菌、志贺毒素产生性大肠埃希氏菌(STEC)及其他食源性致病菌 将结果(和元数据)实时共享给国家生物技术信息中心 研究人员和公共卫生人员可以访问的国家肠道病原体基因组数据库 对官员进行实时比较和分析。 我们预计大多数分离株将是肠道沙门氏菌和弯曲杆菌。就像我们 已经与俄勒冈州立大学建立了工作关系，并获得了 测序。作为NMDA专业作物研究的一部分的当前环境监测活动 NMSU FSL的活动也在产生李斯特菌和沙门氏菌分离株，我们将对其进行测序。 NMSU FSL致力于GenomeTrakr努力创造更强大的国际快速 可有效利用的病原菌聚集性相关食品的监测网络 确定导致有机体的疾病来源，以进一步履行FDA保护公众的使命 健康。

Project Summary Microbiology, Track 4: Whole Genome Sequencing The NMSU Food Safety Laboratory (FSL) will work towards achieving FERN objectives by ensuring that trained staff will participate in FERN activities including sequencing bacterial isolates, participating in proficiency tests, and performing other analyses as requested. The NMSU FSL proposes to sequence a mixture of 400 or more pathogens (Listeria monocytogenes, Salmonella, Shiga-toxin producing Escherichia coli (STEC) and other foodborne related pathogens and share the results (and metadata) in real-time to the National Center for Biotechnology Information national database for enteric pathogen genomes that can be accessed by researchers and public health officials for real time comparison and analysis. We anticipate that the majority of the isolates will be Salmonella enterica and Campylobacter spp. as we have developed a working relationship with Oregon State University and to obtain isolates for sequencing. Current environmental monitoring activities as a part of NMDA Specialty Crop research activities at the NMSU FSL are also generating Listeria and Salmonella isolates that we will sequence. The NMSU FSL is committed to the GenomeTrakr efforts to create a stronger international rapid surveillance network for clustering of pathogen isolates associated foods that can be effectively used for identifying the sources of disease causing organisms to further FDA’s mission of protecting the public health.