Microbiology, Track 4: Whole Genome Sequencing

项目摘要 微生物学，轨道4：全基因组测序 NMSU食品安全实验室（FSL）将努力实现FERN的目标，确保 经过培训的工作人员将参加FERN的活动，包括对细菌分离株进行测序， 能力测试，并根据要求进行其他分析。 NMSU FSL提出对400种或更多种病原体（单核细胞增生李斯特菌， 沙门氏菌、产志贺毒素大肠杆菌（STEC）和其他食源性相关病原体， 将结果（和元数据）实时分享给国家生物技术信息中心 可供研究人员和公共卫生部门访问的肠道病原体基因组国家数据库 官员进行真实的时间比较和分析。 我们预计大多数分离株将是肠道沙门氏菌和弯曲杆菌属。正如我们 已经与俄勒冈州州立大学建立了工作关系，并获得分离物， 测序作为NMDA特种作物研究一部分的当前环境监测活动 NMSU FSL的活动也产生了我们将测序的李斯特菌和沙门氏菌分离株。 NMSU FSL致力于GenomeTrakr努力创造一个更强大的国际快速 监测网络，用于与食品相关的病原体分离物聚类， 确定致病微生物的来源，以进一步履行FDA保护公众的使命 健康

Project Summary Microbiology, Track 4: Whole Genome Sequencing The NMSU Food Safety Laboratory (FSL) will work towards achieving FERN objectives by ensuring that trained staff will participate in FERN activities including sequencing bacterial isolates, participating in proficiency tests, and performing other analyses as requested. The NMSU FSL proposes to sequence a mixture of 400 or more pathogens (Listeria monocytogenes, Salmonella, Shiga-toxin producing Escherichia coli (STEC) and other foodborne related pathogens and share the results (and metadata) in real-time to the National Center for Biotechnology Information national database for enteric pathogen genomes that can be accessed by researchers and public health officials for real time comparison and analysis. We anticipate that the majority of the isolates will be Salmonella enterica and Campylobacter spp. as we have developed a working relationship with Oregon State University and to obtain isolates for sequencing. Current environmental monitoring activities as a part of NMDA Specialty Crop research activities at the NMSU FSL are also generating Listeria and Salmonella isolates that we will sequence. The NMSU FSL is committed to the GenomeTrakr efforts to create a stronger international rapid surveillance network for clustering of pathogen isolates associated foods that can be effectively used for identifying the sources of disease causing organisms to further FDA’s mission of protecting the public health.