Microbiology - Whole Genome Sequencing Analytical Track

提高能力和容量 微生物和化学检测支持 食品防务与食品安全一体化体系 微生物学学科，全基因组测序分析跟踪 项目摘要/摘要 本项目建议书是俄亥俄州政府部门多项目申请的一部分。 回应供资机会公告的农业(官方发展援助)#PAR-20-105 来自食品和药物管理局(FDA)监管事务办公室(ORA)， 兽医(CVM)和食品安全与应用营养中心(CFSAN)。这 联合国开发计划署动物疾病诊断实验室(ADTL)的提案解决了这一关键项目 微生物分析轨道4(全基因组测序(WGS))中描述的区域 美国之音。ADDL准备达到至少400个分离物的高样本负荷 在1年内测序。ADDL利用Illumina MiSeq和ISEQ平台，确保实验室 有能力对人类或动物性食物以及环境中的李斯特氏菌分离株进行排序 单核细胞增多症、沙门氏菌和志贺氏菌毒素产生菌(STEC)。重要的是，2019年 ADL的革兰氏阴性杆菌和革兰氏阴性杆菌的WGS方法通过了国际标准化组织17025认证。 阳性细菌分离株。我们有信心能够实现这个项目的目标，并 期待着有机会与FDA合作。

Increasing the Capability and Capacity of Microbiological and Chemical Testing to Support an Integrated Food Defense and Food Safety System Microbiology Discipline, Whole Genome Sequencing Analytical Track Project Summary / Abstract This Project proposal is part of a Multi Project Application from the Ohio Department of Agriculture (ODA) in response to Funding Opportunity Announcement (FOA) # PAR-20-105 from the Food and Drug Administration’s (FDA) Office of Regulatory Affairs (ORA), Center for Veterinary Medicine (CVM) and Center for Food Safety and Applied Nutrition (CFSAN). This proposal from ODA’s Animal Disease Diagnostic Laboratory (ADDL) addresses the key project areas as described in Microbiology Analytical Track 4 (Whole Genome Sequencing (WGS)) of the FOA. The ADDL is prepared to achieve the ‘High’ Sample Load of at least 400 isolates sequenced in 1 year. The ADDL utilizes the Illumina MiSeq and iSeq platforms, ensuring the lab has the ability to sequence human or animal food, and environmental isolates for Listeria monocytogenes, Salmonella and Shiga toxin-producing E. coli (STEC). Importantly, in 2019 the ADDL achieved ISO 17025 accreditation of its WGS method for gram-negative and gram- positive bacteria isolates. We are confident that we can meet the objectives of this project and look forward to the opportunity to work with the FDA.