Microbiology - Whole Genome Sequencing Analytical Track

微生物学 - 全基因组测序分析轨道

• 批准号: 10173193
• 负责人: YAN ZHANG
• 金额: $ 10.67万
• 依托单位: OHIO STATE DEPARTMENT OF AGRICULTURE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10173193
• 关键词: Microbiology; Whole; Genome; Sequencing; Analytical

Increasing the Capability and Capacity of Microbiological and Chemical Testing to Support an Integrated Food Defense and Food Safety System Microbiology Discipline, Whole Genome Sequencing Analytical Track Project Summary / Abstract This Project proposal is part of a Multi Project Application from the Ohio Department of Agriculture (ODA) in response to Funding Opportunity Announcement (FOA) # PAR-20-105 from the Food and Drug Administration’s (FDA) Office of Regulatory Affairs (ORA), Center for Veterinary Medicine (CVM) and Center for Food Safety and Applied Nutrition (CFSAN). This proposal from ODA’s Animal Disease Diagnostic Laboratory (ADDL) addresses the key project areas as described in Microbiology Analytical Track 4 (Whole Genome Sequencing (WGS)) of the FOA. The ADDL is prepared to achieve the ‘High’ Sample Load of at least 400 isolates sequenced in 1 year. The ADDL utilizes the Illumina MiSeq and iSeq platforms, ensuring the lab has the ability to sequence human or animal food, and environmental isolates for Listeria monocytogenes, Salmonella and Shiga toxin-producing E. coli (STEC). Importantly, in 2019 the ADDL achieved ISO 17025 accreditation of its WGS method for gram-negative and gram- positive bacteria isolates. We are confident that we can meet the objectives of this project and look forward to the opportunity to work with the FDA.

提高能力和能力 微生物和化学测试以支持 综合食品防御和食品安全系统 微生物学学科，整个基因组测序分析轨道 项目摘要 /摘要 该项目提案是俄亥俄州俄亥俄州部的多个项目申请的一部分 农业（ODA）回应资金机会公告（FOA）＃PAR-20-105 来自美国食品药品监督管理局（FDA）监管事务办公室（ORA），中心 兽医医学（CVM）和食品安全与应用营养中心（CFSAN）。这 ODA动物疾病诊断实验室（ADDL）的提案解决了关键项目 微生物学分析轨道4（全基因组测序（WGS））中所述的区域 foa。该ADDL准备达到至少400个分离株的“高”样品负载 在1年内测序。 ADDL利用Illumina Miseq和ISEQ平台，确保实验室 具有对人类或动物食物进行测序的能力，以及李斯特菌的环境分离株 单核细胞增生，沙门氏菌和志贺毒素产生的大肠杆菌（STEC）。重要的是，2019年 ADDL获得了ISO 17025的WGS方法的认证 阳性细菌分离株。我们相信我们可以符合该项目的目标，并且 期待有机会与FDA合作。