Microbiology - Whole Genome Sequencing Analytical Track

提高能力和能力 微生物和化学测试，以支持 综合食品防御和食品安全系统 微生物学学科，全基因组测序分析轨道 项目概要/摘要 本项目提案是俄亥俄州教育部多项目申请的一部分。 农业（ODA）响应资助机会公告（FOA）# PAR-20-105 来自美国食品药品监督管理局（FDA）法规事务办公室（ORA）， 兽医（CVM）和食品安全和应用营养中心（CFSAN）。这 ODA的动物疾病诊断实验室（ADDL）提出了一项关键项目， 微生物学分析轨道4（全基因组测序（WGS）） 的FOA。制备ADDL以达到至少400株分离株的“高”样本上样量 1年内完成测序。ADDL利用Illumina MiSeq和iSeq平台，确保实验室 能够对人类或动物食品以及李斯特菌的环境分离株进行测序 产单核细胞增多症、沙门氏菌和产滋贺毒素E. coli（STEC）。重要的是，在2019年， ADDL获得了ISO 17025认证，其WGS方法用于革兰氏阴性和革兰氏阴性 阳性细菌分离物。我们相信，我们能够实现这一项目的目标， 我期待着与FDA合作的机会。

Increasing the Capability and Capacity of Microbiological and Chemical Testing to Support an Integrated Food Defense and Food Safety System Microbiology Discipline, Whole Genome Sequencing Analytical Track Project Summary / Abstract This Project proposal is part of a Multi Project Application from the Ohio Department of Agriculture (ODA) in response to Funding Opportunity Announcement (FOA) # PAR-20-105 from the Food and Drug Administration’s (FDA) Office of Regulatory Affairs (ORA), Center for Veterinary Medicine (CVM) and Center for Food Safety and Applied Nutrition (CFSAN). This proposal from ODA’s Animal Disease Diagnostic Laboratory (ADDL) addresses the key project areas as described in Microbiology Analytical Track 4 (Whole Genome Sequencing (WGS)) of the FOA. The ADDL is prepared to achieve the ‘High’ Sample Load of at least 400 isolates sequenced in 1 year. The ADDL utilizes the Illumina MiSeq and iSeq platforms, ensuring the lab has the ability to sequence human or animal food, and environmental isolates for Listeria monocytogenes, Salmonella and Shiga toxin-producing E. coli (STEC). Importantly, in 2019 the ADDL achieved ISO 17025 accreditation of its WGS method for gram-negative and gram- positive bacteria isolates. We are confident that we can meet the objectives of this project and look forward to the opportunity to work with the FDA.