Microbiology Capability and Capacity Development

罗德岛州PAR-20-105实验室灵活资助模式项目总结 (LFFM)(U19)申请。学科A：微生物学，轨道5：微生物学 能力/能力开发 项目摘要 罗德岛州卫生实验室(RISHL)将扩大其ISO/IEC的范围 17025：通过开发方法能力来测试2017年认可的食品测试 根据FDA BAM，人类食品和环境样品中的环孢子菌 方法19b。RISHL将依靠该项目的支持来获得设备和 供应品包括均质机、平台摇杆、摆动铲斗转子和带 锥形管的插入物和其他设备是准备样品以供分析所必需的 FDA BAM方法19b：生鲜农产品中卡氏环孢霉的分子检测 使用实时荧光定量聚合酶链式反应。该项目的支持还将用于购买Primer和 探索两个目标(环孢菌素和IAC)，以培训FDA BAM方法19b的人员 并制定实验室的标准操作程序。RISHL将参加 成功完成由合适的熟练程度提供者提供的熟练程度学习或在 实验室制定了证明能力的练习。 1

Project Summary for State of Rhode Island PAR-20-105 Laboratory Flexible Funding Model (LFFM) (U19) Application. Discipline A: Microbiology, Track 5: Microbiology Capability/Capacity Development Project Summary The Rhode Island State Health Laboratories (RISHL) will expand its scope of ISO/IEC 17025:2017 accredited food testing by developing the method capability to test for Cyclospora in human food product and environmental samples according to FDA BAM method 19b. The RISHL will rely on the support of this project to acquire the equipment and supplies including a homogenizer, platform rocker, swinging bucket rotor and buckets with inserts for conical tubes, and other equipment an necessary to prepare samples for analysis by FDA BAM method 19b: Molecular Detection of Cyclospora cayetanensis in Fresh Produce Using Real-Time PCR. Support from this project will also be used to acquire Primer and Probes for two targets (Cyclospora and IAC), to train personnel on FDA BAM method 19b and to develop Standard Operating Procedures for the laboratory. The RISHL will participate successfully in a proficiency study offered by a suitable proficiency provider or in a laboratory developed exercise to demonstrate competency. 1