Microbiology: Analytical Track 5: Microbiology Capability/Capacity Development 2020-2021

夏威夷州立卫生局 国家实验室分部 实验室准备和反应处 生物反应科 学科A-分析轨道5：微生物学能力/能力发展--环孢菌科 项目总结： 环孢子虫病又称“旅行者腹泻”，是由原生动物寄生虫--环孢子虫引起的 卡亚坦人。感染是通过摄入粪便污染的食物或食物中的感染卵囊而获得的 水。美国的环孢子虫病经常与进口新鲜食品的消费有关 包括绿叶蔬菜和浆果在内的农产品。自20世纪90年代中期以来，美国陆续报告了S病例 国家通常与进口新鲜农产品的消费挂钩。2013-2015年，美国疾病控制与预防中心 美国疾病控制和预防中心(CDC)报告称，在多个州爆发的C。 卡亚坦人。这场多个州的疫情与墨西哥普埃布拉种植的新鲜香菜有关。从2016年开始- 2019年，美国统计数据显示，与多个州的爆发和聚集性有关的环孢子虫病急剧增加 感染的可能性。 根据夏威夷大学希洛分校为夏威夷分部编制的2016年报告 在农业方面，夏威夷85%的食品供应是国内和国际进口的。这使得 夏威夷容易受到食源性疾病的影响。夏威夷一名居民未发现环孢子虫病病例 在过去的十年里。夏威夷没有病例可能部分是因为没有进行敏感的检测 检查粪便样本中是否有卡氏梭菌。 这项提议的目标是在HI-DOH州建立环孢子菌食品检测能力 实验室科(SLD)蕨类微生物学实验室。希望通过发展这一能力， 检测可以在州内进行，而不需要送到最近的FDA办公室，那里有五个小时的飞机路程。 在状态下测试食品样品的能力将缩短周转时间(TAT)，结果可以支持 为干预和缓解目的进行的调查。此外，对食品进行检测的能力 环孢子虫可帮助对可能病例进行流行病学调查。既有能力测试临床 粪便样本和检测可能受污染的食品将有助于建立流行病学联系 并有助于更有效地追踪案件。

Hawaii State Department of Health State Laboratories Division Laboratory Preparedness and Response Branch Biological Response Section Discipline A – Analytical Track 5: Microbiology Capability/Capacity Development - Cyclospora Project Summary: Cyclosporiasis otherwise known as "traveler's diarrhea" is caused by a protozoan parasite, Cyclospora cayatanensis. The infection is acquired by the ingestion of infective oocysts from fecal-contaminated food or water. Cyclosporiasis in the United States has been frequently linked to consumption of imported fresh produce including leafy greens and berries. Since the mid-1990's cases have been reported in the United States usually linked to consumption of imported fresh produce. In 2013-2015, the Centers for Disease Control and Prevention (CDC) reported more than 1400 cases in a multi-state outbreak of infection due to C. cayatanensis. This multi-state outbreak was linked to fresh cilantro grown in Puebla, Mexico. From 2016- 2019, US statistics showed a dramatic increase of Cyclosporiasis linked to multi-states outbreaks and clusters of infection. According to a 2016 report prepared by the University of Hawaii – Hilo for the Hawaii Department of Agriculture, eighty five percent of food supply in Hawaii is imported, nationally and internationally. This makes Hawaii vulnerable to food-borne illnesses. No cases of cyclosporiasis have been identified in a Hawaii resident in the past ten years. The absence of cases in Hawaii maybe due in part to absence of sensitive tests to examine stool samples for C. cayatanenesis. The objective of this proposal is to establish Cyclospora food testing capability in the HI-DOH State Laboratories Division (SLD) FERN Microbiology laboratory. It is hoped that by developing this capability, testing can be done in-state without the need to send to the nearest FDA Office which is five hours away by air. The ability to test food samples in-state will shorten Turn-Around-Time (TAT) and results can support investigations for intervention and mitigation purposes. Additionally, the ability to test food products for Cyclospora can aid in epidemiological investigations of possible cases. Having both the ability to test clinical stool samples and test potentially contaminated food products will aid in establishing an epidemiological link and aid in a more efficient tracing of cases.