Microbiology: Analytical Track 5: Microbiology Capability/Capacity Development 2020-2021

夏威夷州卫生部 国家实验室司 实验室准备和反应分支 生物反应科 学科A -分析轨道5：微生物学能力/能力发展-环孢菌 项目摘要： 环孢菌病又称旅行者腹泻，是由一种原生寄生虫环孢菌引起的 cayatanensis。感染是通过从粪便污染的食物中摄入感染性卵囊获得的， 水在美国，环孢霉经常与进口新鲜食品的消费联系在一起。 农产品包括绿叶蔬菜和浆果。自20世纪90年代中期以来，在美国已经报告了病例。 各国通常与进口新鲜农产品的消费联系在一起。在2013-2015年，疾病中心 美国疾病控制和预防中心（CDC）报告了1400多例多州爆发的C。 cayatanensis。这次多州爆发与墨西哥普埃布拉种植的新鲜香菜有关。从2016年开始- 2019年，美国统计数据显示，与多个州爆发和集群有关的环孢菌素急剧增加 感染 根据夏威夷大学希洛分校为夏威夷大学教育部编写的2016年报告， 农业，夏威夷百分之八十五的食品供应是进口的，国内和国际。这使得 夏威夷易受食源性疾病的影响。在夏威夷居民中没有发现环孢菌素病例 在过去的十年里。夏威夷没有病例，部分原因可能是缺乏敏感的测试， 检查粪便样本中是否有C。卡亚塔演化 本提案的目的是在HI-DOH州建立环孢子虫食品检测能力 实验室分部（SLD）FERN微生物学实验室。希望通过发展这种能力， 测试可以在州内完成，而不需要送到最近的FDA办公室，这是五个小时的空中路程。 在州内测试食品样品的能力将缩短周转时间（达特），结果可以支持 为干预和缓解目的进行调查。此外，测试食品的能力， 环孢子虫可以帮助对可能的病例进行流行病学调查。既有能力测试临床 粪便样本和测试可能受污染的食品将有助于建立流行病学联系 并有助于更有效地追踪案件。

Hawaii State Department of Health State Laboratories Division Laboratory Preparedness and Response Branch Biological Response Section Discipline A – Analytical Track 5: Microbiology Capability/Capacity Development - Cyclospora Project Summary: Cyclosporiasis otherwise known as "traveler's diarrhea" is caused by a protozoan parasite, Cyclospora cayatanensis. The infection is acquired by the ingestion of infective oocysts from fecal-contaminated food or water. Cyclosporiasis in the United States has been frequently linked to consumption of imported fresh produce including leafy greens and berries. Since the mid-1990's cases have been reported in the United States usually linked to consumption of imported fresh produce. In 2013-2015, the Centers for Disease Control and Prevention (CDC) reported more than 1400 cases in a multi-state outbreak of infection due to C. cayatanensis. This multi-state outbreak was linked to fresh cilantro grown in Puebla, Mexico. From 2016- 2019, US statistics showed a dramatic increase of Cyclosporiasis linked to multi-states outbreaks and clusters of infection. According to a 2016 report prepared by the University of Hawaii – Hilo for the Hawaii Department of Agriculture, eighty five percent of food supply in Hawaii is imported, nationally and internationally. This makes Hawaii vulnerable to food-borne illnesses. No cases of cyclosporiasis have been identified in a Hawaii resident in the past ten years. The absence of cases in Hawaii maybe due in part to absence of sensitive tests to examine stool samples for C. cayatanenesis. The objective of this proposal is to establish Cyclospora food testing capability in the HI-DOH State Laboratories Division (SLD) FERN Microbiology laboratory. It is hoped that by developing this capability, testing can be done in-state without the need to send to the nearest FDA Office which is five hours away by air. The ability to test food samples in-state will shorten Turn-Around-Time (TAT) and results can support investigations for intervention and mitigation purposes. Additionally, the ability to test food products for Cyclospora can aid in epidemiological investigations of possible cases. Having both the ability to test clinical stool samples and test potentially contaminated food products will aid in establishing an epidemiological link and aid in a more efficient tracing of cases.