Capacity Development - Nanopore MinION

而Illumina平台是全基因组测序中应用最广泛的技术 新兴技术(WGS)展现出令人振奋的前景。因为牛津纳米孔 技术产生了非常长的读数，有可能‘关闭’基因组，识别染色体 从质粒中分离出来。将生物信息学工具与识别等位基因的工具结合起来关闭基因组 对AR条件的贡献，将有可能开发一条生物信息学管道来确定 等位基因在基因组中的位置。西澳PHL计划开发这些工具，以及一个 在AR分析中常规使用附属物的实验室协议，并验证这些路径 临床报告。WAPHL认为使该项目可行的几个关键因素包括两个 已经接受过技术培训的微生物学家、常驻生物信息学家、AWS帐户 作为开发分析工具的环境，几位微生物学家已经在 WGS的概念。

While the Illumina platform is the most widely used technology in whole genome sequencing (WGS), an emerging technology shows exciting promise. Because the Oxford Nanopore technology produces very long reads, it is possible to ‘close’ genomes, identifying chromosomes from plasmids. Combining bioinformatics tools to close the genome with tools to identify alleles contributing to AR conditions, it will be possible to develop a bioinformatics pipeline to determine the location of the alleles within a genome. WA PHL plans to develop these tools along with a laboratory protocol for routine use of the MinION in AR analyses, and validate these paths for clinical reporting. WAPHL holds several key elements making this project feasible, including two microbiologists already trained in the technology, a resident bioinformaticist, an AWS account as an environment to develop analysis tools, and several microbiologists already trained in the concepts of WGS.