Capacity Development - Nanopore MinION

能力发展 - Nanopore MinION

• 批准号: 10173519
• 负责人: Philip Dykema
• 金额: $ 12.74万
• 依托单位: WASHINGTON STATE DEPARTMENT OF HEALTH
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10173519
• 关键词: Capacity; Development; Nanopore; MinION

While the Illumina platform is the most widely used technology in whole genome sequencing (WGS), an emerging technology shows exciting promise. Because the Oxford Nanopore technology produces very long reads, it is possible to ‘close’ genomes, identifying chromosomes from plasmids. Combining bioinformatics tools to close the genome with tools to identify alleles contributing to AR conditions, it will be possible to develop a bioinformatics pipeline to determine the location of the alleles within a genome. WA PHL plans to develop these tools along with a laboratory protocol for routine use of the MinION in AR analyses, and validate these paths for clinical reporting. WAPHL holds several key elements making this project feasible, including two microbiologists already trained in the technology, a resident bioinformaticist, an AWS account as an environment to develop analysis tools, and several microbiologists already trained in the concepts of WGS.

虽然Illumina平台是全基因组测序中使用最广泛的技术， (WGS)一项新兴技术显示出令人兴奋的前景。因为牛津纳米孔 技术产生非常长的读数，有可能“关闭”基因组，识别染色体， from plasmid质粒.将关闭基因组的生物信息学工具与识别等位基因的工具相结合 有助于AR条件，将有可能开发一个生物信息学管道，以确定 等位基因在基因组中的位置。WA PHL计划沿着开发这些工具， 在AR分析中常规使用MinION的实验室方案，并验证这些路径， 临床报告WAPHL拥有使该项目可行的几个关键因素，包括两个 已经接受过这项技术培训的微生物学家、常驻生物信息学家、AWS账户 作为开发分析工具的环境，几位微生物学家已经接受了 WGS的概念