Monitoring antimicrobial resistance (AMR) in enteric pathogens isolated from retail meats and seafoods in North Carolina

项目摘要 食用动物，包括零售猪肉、鸡肉、火鸡肉和牛肉， 它们被认为是沙门氏菌和弯曲杆菌的来源，并且是细菌感染的主要原因。 食源性疾病无论是野生捕捞还是养殖的鲑鱼、虾和罗非鱼， 被认为是弧菌和气单胞菌的来源，是海洋食源性疾病的主要原因。 病指示病原体，包括大肠杆菌和肠球菌属，也 经常从这些肉类产品中分离出来。情况变得更加复杂， 菌株还对不同的抗微生物剂具有抗性。因此，我们必须监察 零售业中抗生素耐药（AMR）食源性细菌菌株的流行和趋势 肉类和海鲜产品，最终可以通过沿着农场到餐桌的消费者 链造成重大公共卫生影响。国家抗菌药物耐药性 抗生素耐药性监测系统（NARMS）在抗生素耐药性细菌病原体监测中发挥着重要作用 在人类、食用动物和零售肉类中。北卡罗来纳州是一个重要的粮食生产州 位于美国东部海岸沿着，是猪肉和家禽产量最高的州之一 生产作为现有的NARMS实验室，我们的主要目标是继续提高 香港零售肉类及海产品中抗生素耐药性致病菌及指示菌的监测 北卡罗来纳州，并产生关键数据，以促进NARMS使命。我们建议 与FDA GenomeTrakr项目合作开展本研究。主要研究者（Thakur博士） 实验室是一个GenomeTrakr实验室，它将提供机会，以产生 细菌菌株的全基因组序列（WGS），从而显著帮助我们 跟踪潜在的新AMR细菌的出现，并允许公共卫生机构 采取适当的措施。 我们建议的具体目标是： 1)监测沙门氏菌、弯曲杆菌、 大肠杆菌、弧菌、气单胞菌、乳糖发酵菌和肠球菌属。从零售肉类 和海鲜样本收集在北卡罗来纳州地区。 2)生成并比较沙门氏菌、弯曲杆菌和大肠杆菌的WGS图谱。大肠杆菌， 从北卡罗来纳州的零售肉类中分离，以检测新AMR菌株的出现。 我们建议的长远目标，是透过加强 加强AMR沙门氏菌、弯曲杆菌、大肠杆菌的监测。大肠杆菌，弧菌， 气单胞菌、乳糖发酵菌和肠球菌属。在美国的零售食品样品中。

Project Summary Food animals, including retail pork, chicken, ground turkey and ground beef have been implicated as sources of Salmonella and Campylobacter and are the leading causes of bacterial foodborne illness. Both wild-caught and farm-raised salmon, shrimp, and tilapia have been implicated as sources of Vibrio and Aeromonas and are leading causes of sea-foodborne illness. Indicator pathogens, including Escherichia coli and Enterococcus spp., are also frequently isolated from these meat products. The situation is further complicated when the strains are also resistant to different antimicrobials. It is therefore important that we monitor the prevalence and trends of antimicrobial resistant (AMR) foodborne bacterial strains in the retail meat and seafood products that can eventually pass to the consumers along the farm-to-fork chain resulting in significant public health impact. The National Antimicrobial Resistance Monitoring System (NARMS) plays an important role in surveillance of AMR bacterial pathogens in humans, food animals and retail meats. North Carolina is an important food producing state situated along the eastern US seashore and ranks among the top states in pork and poultry production. As an existing NARMS laboratory, our major objective is to continue to enhance the surveillance of AMR pathogenic and indicator bacterial strains in retail meats and seafood in North Carolina and generate critical data to contribute to the NARMS mission. We propose to conduct this study in collaboration with the FDA GenomeTrakr program. The PI (Dr. Thakur) laboratory is a GenomeTrakr laboratory, which will provide the opportunity to generate the whole genome sequences (WGS) of the bacterial strains thereby significantly assisting us in tracking the emergence of potential new AMR bacteria and allowing the public health agencies to take appropriate steps. The specific objectives of our proposal are: 1) Monitor antimicrobial resistance (AMR) trends in Salmonella, Campylobacter, Escherichia coli, Vibrio, Aeromonas, lactose fermenters, and Enterococcus spp. from retail meat and seafood samples collected in North Carolina region. 2) Generate and compare the WGS profiles of Salmonella, Campylobacter and E. coli, isolated from retail meat in North Carolina to detect the emergence of new AMR strains. The long-term objective of our proposal is to protect and promote public health by enhancing and strengthening the surveillance of AMR Salmonella, Campylobacter, E. coli, Vibrio, Aeromonas, lactose fermenters, and Enterococcus spp. in retail food specimens in the US.