Monitoring antimicrobial resistance (AMR) in enteric pathogens isolated from retail meats and seafoods in North Carolina

项目摘要 食用动物，包括零售猪肉、鸡肉、碎火鸡和碎牛肉 与沙门氏菌和弯曲杆菌的来源有关，是细菌的主要原因 食源性疾病。无论是野生捕捞的还是养殖的鲑鱼、虾和罗非鱼都 被认为是弧菌和气单胞菌的来源，是海源性食品的主要原因 生病了。指示病原体，包括大肠埃希氏菌和肠球菌，也是 经常从这些肉制品中分离出来。情况进一步复杂化，因为 菌株对不同的抗菌剂也有抗药性。因此，重要的是我们要监测 零售业食源性耐药(AMR)菌株的流行及趋势 肉类和海鲜产品最终可以通过从农场到餐桌的方式传递给消费者 造成重大公共卫生影响的链条。国家抗菌素耐药性 监测系统(NARMS)在AMR细菌病原菌监测中发挥着重要作用 在人类、食用动物和零售肉类中。北卡罗来纳州是一个重要的粮食生产州。 位于美国东部海滨，是猪肉和家禽的顶级州之一 制作。作为现有的NARMS实验室，我们的主要目标是继续加强 年零售肉类和海产品中AMR病原菌和指示菌的监测 并生成关键数据，为NARMS任务做出贡献。我们建议 与FDA GenomeTrakr计划合作进行这项研究。少年派(塔库尔博士) 实验室是GenomeTrakr实验室，它将提供产生 细菌菌株的全基因组序列(WGS)，从而大大帮助我们 跟踪潜在的新AMR细菌的出现，并允许公共卫生机构 采取适当的措施。 我们建议的具体目标是： 1)监测沙门氏菌、弯曲杆菌、 大肠埃希氏菌、弧菌、气单胞菌、乳糖发酵菌和肠球菌。来自零售肉类 以及在北卡罗来纳州地区采集的海鲜样本。 2)生成并比较沙门氏菌、弯曲杆菌和大肠杆菌的WGS图谱， 从北卡罗来纳州零售肉类中分离出来，以检测新的AMR菌株的出现。 我们建议的长远目标，是透过加强 加强对耐甲氧西林沙门氏菌、弯曲杆菌、大肠杆菌、弧菌、 气单胞菌、乳糖发酵剂和肠球菌。在美国的零售食品样本中。

Project Summary Food animals, including retail pork, chicken, ground turkey and ground beef have been implicated as sources of Salmonella and Campylobacter and are the leading causes of bacterial foodborne illness. Both wild-caught and farm-raised salmon, shrimp, and tilapia have been implicated as sources of Vibrio and Aeromonas and are leading causes of sea-foodborne illness. Indicator pathogens, including Escherichia coli and Enterococcus spp., are also frequently isolated from these meat products. The situation is further complicated when the strains are also resistant to different antimicrobials. It is therefore important that we monitor the prevalence and trends of antimicrobial resistant (AMR) foodborne bacterial strains in the retail meat and seafood products that can eventually pass to the consumers along the farm-to-fork chain resulting in significant public health impact. The National Antimicrobial Resistance Monitoring System (NARMS) plays an important role in surveillance of AMR bacterial pathogens in humans, food animals and retail meats. North Carolina is an important food producing state situated along the eastern US seashore and ranks among the top states in pork and poultry production. As an existing NARMS laboratory, our major objective is to continue to enhance the surveillance of AMR pathogenic and indicator bacterial strains in retail meats and seafood in North Carolina and generate critical data to contribute to the NARMS mission. We propose to conduct this study in collaboration with the FDA GenomeTrakr program. The PI (Dr. Thakur) laboratory is a GenomeTrakr laboratory, which will provide the opportunity to generate the whole genome sequences (WGS) of the bacterial strains thereby significantly assisting us in tracking the emergence of potential new AMR bacteria and allowing the public health agencies to take appropriate steps. The specific objectives of our proposal are: 1) Monitor antimicrobial resistance (AMR) trends in Salmonella, Campylobacter, Escherichia coli, Vibrio, Aeromonas, lactose fermenters, and Enterococcus spp. from retail meat and seafood samples collected in North Carolina region. 2) Generate and compare the WGS profiles of Salmonella, Campylobacter and E. coli, isolated from retail meat in North Carolina to detect the emergence of new AMR strains. The long-term objective of our proposal is to protect and promote public health by enhancing and strengthening the surveillance of AMR Salmonella, Campylobacter, E. coli, Vibrio, Aeromonas, lactose fermenters, and Enterococcus spp. in retail food specimens in the US.