Enhance the surveillance of foodborne bacterial pathogens isolated from food and environmental samples using whole genome sequencing (WGS) to prevent foodborne outbreaks and promote public health

摘要：总体 自2016年以来，北卡罗来纳州立大学(NCSU)帮助扩大了GenomeTrakr计划在全国 并在全球范围内针对世界范围内食源性疾病的一些主要原因。监视的必要性 当务之急是控制食源性病原体，因为尽管在卫生、食品和水质以及病原体方面取得了进展 尽管如此，食源性疾病仍然是公共卫生的巨大负担。环境与食品 如零售肉类被牵连为食源性病原体的来源，并建立标准化 对这些来源的监测可以帮助监测关键病原体并追踪污染途径，以防止和 控制疫情。到目前为止，NCSU GenomeTrakr实验室已经产生了3328个全基因组 从多个来源分离的细菌病原体的测序(WGS)图谱。这包括配置文件 在过去三年中，来自美国以外9个国家的1,127个细菌分离株。他们还培训了23名 来自10个国家的学生和专业人士，这导致了GenomeTrakr的11种原创出版物 由研究人员及其美国和国际研究人员共同撰写的同行评议期刊中的项目数据 实习生。自2016年以来，调查人员一直在努力扩大他们的全球网络，现在包括13个国家 横跨三大洲。因此，该项目的总体目标是继续加强WGS-- 在国家和全球一级监测与食源性疾病有关的细菌病原体 一个健康的方法。这将通过监测活动、建立伙伴关系和培训来实现。 学生和专业人士。调查人员将利用他们在监视方面的丰富领导经验 方案、先进分子技术和数据分析及其作为世卫组织合作中心的地位 AMR和One Health通过为学生和 专业人士。为了实现这一目标，他们将追求以下具体目标。目标1(项目)：扩展现有 从食品和食品中分离出的与食源性疾病有关的细菌病原体监测系统 在美国和国际地点的环境来源。目标2(项目)：比较细菌的WGS图谱 从国家和国际样本中分离出病原体，以推进疫情调查和跟踪 新出现的对公共卫生具有重要意义的菌株。这项工作将得到行政核心的两个目标的支持： 目标1(行政)：与国家和国际合作，领导完成拟议的目标 国际机构。目标2(行政)：提供一个框架，以教育和装备未来的监测 拥有WGS分析所需知识和技能的员工队伍。预期结果：调查人员是 致力于完成GenomeTrakr的使命并将标准化监控系统扩展到整个 环球网。通过从新的和现有的合作者那里鉴定来自美国和国际来源的400个新的分离物， 通过在全球范围内建设能力，这项工作将帮助公共卫生机构追踪新菌株的出现， 遏制食源性病原体的传播，减轻美国和全球食源性疾病的负担。

Summary: Overall Since 2016, North Carolina State University (NCSU) has helped expand the GenomeTrakr program on a national and global scale to target some of the leading causes of foodborne illnesses worldwide. The need for surveillance of foodborne pathogens is urgent because despite advances in hygiene, food and water quality and pathogen detection, foodborne illnesses remain a tremendous burden for public health. The environment and food products such as retail meats are implicated as sources of foodborne pathogens, and establishing standardized surveillance of these sources can help monitor key pathogens and trace contamination routes to prevent and control outbreaks. To date, NCSU GenomeTrakr laboratories have generated 3,328 whole genome sequencing (WGS) profiles of bacterial pathogens isolated from multiple sources. This includes profiles of 1,127 bacterial isolates from 9 countries outside the US over the last three years. They have also trained 23 students and professionals from 10 countries, which has led to 11 original publications of GenomeTrakr program data in peer-reviewed journals coauthored by the investigators and their US and international trainees. Since 2016, the investigators have worked to expand their global network to now include 13 countries spanning three continents. Consequently, the overarching aim of this project is to continue enhancing WGS- based surveillance of bacterial pathogens associated with foodborne illness at the national and global level using a One Health approach. This will be achieved through surveillance activities, partnership building and training students and professionals. The investigators will leverage their extensive leadership experience in surveillance protocols, advanced molecular techniques and data analysis, and their status as a WHO Collaborating Center for AMR and One Health to build capacity in the US and globally through world-class training for students and professionals. To achieve this, they will pursue the following specific aims. Aim 1 (Project): Expand the existing surveillance system of bacterial pathogens related to foodborne illness that have been isolated from food and environmental sources in the US and international locations. Aim 2 (Project): Compare WGS profiles of bacterial pathogens isolated from national and international samples to advance outbreak investigations and track emerging strains of public health importance. This work will be supported by two aims of the Administrative Core: Aim 1 (Admin): Provide leadership to complete the proposed aims in collaboration with national and international institutions. Aim 2 (Admin): Provide a framework to educate and equip the future surveillance workforce with the knowledge and skills needed for WGS analysis. Expected Outcomes: The investigators are committed to fulfilling the GenomeTrakr mission and expanding standardized surveillance systems across the globe. By characterizing 400 new isolates from US and international sources from new and existing collaborators, and by building capacity worldwide, this work will help public health agencies track the emergence of new strains, curb the distribution of foodborne pathogens and reduce the burden of foodborne illnesses in the US and globally.