Enhance the surveillance of foodborne bacterial pathogens isolated from food and environmental samples using whole genome sequencing (WGS) to prevent foodborne outbreaks and promote public health

利用全基因组测序（WGS）加强对从食品和环境样本中分离出的食源性细菌病原体的监测，以预防食源性疫情并促进公共卫生

• 批准号: 10256644
• 负责人: Siddhartha Thakur
• 金额: $ 17.77万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10256644
• 关键词: Enhance; surveillance; foodborne; bacterial; pathogens

Summary: Overall Since 2016, North Carolina State University (NCSU) has helped expand the GenomeTrakr program on a national and global scale to target some of the leading causes of foodborne illnesses worldwide. The need for surveillance of foodborne pathogens is urgent because despite advances in hygiene, food and water quality and pathogen detection, foodborne illnesses remain a tremendous burden for public health. The environment and food products such as retail meats are implicated as sources of foodborne pathogens, and establishing standardized surveillance of these sources can help monitor key pathogens and trace contamination routes to prevent and control outbreaks. To date, NCSU GenomeTrakr laboratories have generated 3,328 whole genome sequencing (WGS) profiles of bacterial pathogens isolated from multiple sources. This includes profiles of 1,127 bacterial isolates from 9 countries outside the US over the last three years. They have also trained 23 students and professionals from 10 countries, which has led to 11 original publications of GenomeTrakr program data in peer-reviewed journals coauthored by the investigators and their US and international trainees. Since 2016, the investigators have worked to expand their global network to now include 13 countries spanning three continents. Consequently, the overarching aim of this project is to continue enhancing WGS- based surveillance of bacterial pathogens associated with foodborne illness at the national and global level using a One Health approach. This will be achieved through surveillance activities, partnership building and training students and professionals. The investigators will leverage their extensive leadership experience in surveillance protocols, advanced molecular techniques and data analysis, and their status as a WHO Collaborating Center for AMR and One Health to build capacity in the US and globally through world-class training for students and professionals. To achieve this, they will pursue the following specific aims. Aim 1 (Project): Expand the existing surveillance system of bacterial pathogens related to foodborne illness that have been isolated from food and environmental sources in the US and international locations. Aim 2 (Project): Compare WGS profiles of bacterial pathogens isolated from national and international samples to advance outbreak investigations and track emerging strains of public health importance. This work will be supported by two aims of the Administrative Core: Aim 1 (Admin): Provide leadership to complete the proposed aims in collaboration with national and international institutions. Aim 2 (Admin): Provide a framework to educate and equip the future surveillance workforce with the knowledge and skills needed for WGS analysis. Expected Outcomes: The investigators are committed to fulfilling the GenomeTrakr mission and expanding standardized surveillance systems across the globe. By characterizing 400 new isolates from US and international sources from new and existing collaborators, and by building capacity worldwide, this work will help public health agencies track the emergence of new strains, curb the distribution of foodborne pathogens and reduce the burden of foodborne illnesses in the US and globally.

摘要：总体 自2016年以来，北卡罗来纳州州立大学（NCSU）帮助扩大了全国范围内的GenomeTrakr计划。 在全球范围内，针对全球食源性疾病的一些主要原因。监视的必要性 食源性病原体是紧迫的，因为尽管在卫生，食品和水的质量和病原体的进步 尽管食源性疾病没有得到检测，但它仍然是公共卫生的一个巨大负担。环境与食品 例如零售肉类是食源性病原体的来源， 对这些来源的监测有助于监测关键病原体和追踪污染途径， 控制疫情。迄今为止，NCSU GenomeTrakr实验室已生成3，328个全基因组 从多个来源分离的细菌病原体的WGS谱。这包括配置文件 在过去三年中，来自美国以外的9个国家的1，127个细菌分离株。他们还训练了23名 来自10个国家的学生和专业人士，这导致了GenomeTrakr的11个原始出版物 研究人员及其美国和国际同行评审期刊上的项目数据 实习生自2016年以来，调查人员一直致力于扩大其全球网络，目前已包括13个国家 横跨三大洲因此，本项目的总体目标是继续加强WGS- 在国家和全球一级监测与食源性疾病相关的细菌病原体， a“一个健康”办法。这将通过监测活动、建立伙伴关系和培训来实现 学生和专业人士。调查人员将利用他们在监视方面的丰富领导经验 协议、先进的分子技术和数据分析，及其作为世卫组织合作中心的地位 AMR和One Health通过为学生提供世界一流的培训， 专业人士为实现这一目标，他们将努力实现以下具体目标。目标1（项目）：扩大现有的 从食物中分离出的与食源性疾病有关的细菌病原体的监测系统， 美国和国际地区的环境污染源。目的2（项目）：比较细菌的WGS谱 从国家和国际样本中分离出的病原体，以推进疫情调查和跟踪 新出现的对公共卫生具有重要意义的菌株。这项工作将得到行政核心的两个目标的支持： 目标1（行政管理）：与国家和国际组织合作， 国际机构。目标2（管理）：提供一个框架，以教育和装备未来的监测 具备WGS分析所需知识和技能的员工队伍。预期结果：研究者 致力于完成GenomeTrakr的使命，并在全球范围内扩展标准化监测系统。 地球仪。通过表征来自新的和现有的合作者的来自美国和国际来源的400个新分离株， 通过在全球范围内建立能力，这项工作将有助于公共卫生机构跟踪新菌株的出现， 遏制食源性病原体的传播，减轻美国和全球食源性疾病的负担。