COVID19 Slide-seq

COVID19 幻灯片序列

• 批准号: 10173485
• 负责人: Fei Chen
• 金额: $ 17.37万
• 依托单位: BROAD INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-08 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10173485
• 关键词: 2019-nCoV; Adopted; Adoption; Affect; Age; Aging; Archives; Atlases; Autopsy; Bar Codes; COVID-19; COVID-19 pandemic; Cell Nucleus; Cells; Collection; Communities; DNA; Data; Data Collection; Data Set; Development; Disease; Disease Outbreaks; Ensure; Environmental Wind; Freezing; Future; Gene Expression; Genes; Grant; Group Processes; Health; Heart; Hospitals; Human; Image; Infection; Inflammatory; Lead; Libraries; Location; Lung; Molecular; Organ; Organ failure; Pathogenesis; Pathologic; Pathology; Patients; Pattern; Process; Proteins; Publishing; RNA; Regulatory Element; Research; Resolution; Risk; Sampling; Severity of illness; Slide; Smoking Status; Structure; Structure of parenchyma of lung; Technology; Tissues; Update; Virus; Work; biosafety level 3 facility; cell type; disease transmission; experience; gene discovery; genomic platform; genomic tools; global health; human tissue; image processing; in vivo; mortality; multimodality; new technology; novel; oligo (dT); open source; prenatal; programs; response; sex; targeted treatment; tissue resource; tool; transcriptome sequencing; transcriptomics; two-dimensional

Project Summary Cells are the unit building blocks of tissues and organs, thus, to understand how tissues function, it is important to understand where different types of cells reside in health and disease. In this proposal, we propose to develop a novel spatial genomics platform that will enable multi-modal characterization of tissue structure at the single cell level. This will be revolutionary for the study of tissue organization in development and pathological changes in aging and disease. Our tools will enable us to look at RNA, proteins and regulatory elements in DNA all within the tissue context. We will ensure these novel genomic tools are easy to use and adopt, which will be especially powerful for pathological settings, where tissue resources are precious, and adoption of tools is limited by ease of use. In addition, we are applying this technology to rapidly develop a deep molecular and spatial understanding of COVID19 infection and its impacts in organs throughout the body. The successful completion of this work will lead to a comprehensive toolset for understanding tissue structure from the perspective of single cell organization.

项目摘要 细胞是组织和器官的基本单位，因此，要了解组织是如何 功能，重要的是要了解不同类型的细胞居住在健康和 疾病在这个提议中，我们建议开发一个新的空间基因组学平台， 能够在单细胞水平上对组织结构进行多模态表征。这将 对于发育和病理学中的组织组织研究是革命性的， 衰老和疾病的变化。我们的工具将使我们能够观察RNA、蛋白质和调控蛋白质。 DNA中的所有元素都在组织环境中。我们将确保这些新的基因组工具 易于使用和采用，这将是特别强大的病理设置，其中组织 资源是宝贵的，工具的采用受到易用性的限制。此外，我们在 应用这项技术，快速发展深入的分子和空间理解， COVID 19感染及其对全身器官的影响。成功 这项工作的完成将导致一个全面的工具集，了解组织 从单细胞组织的角度来看结构。