A single cell spatial genomics platform for multi-modal characterization of tissue organization

用于组织组织多模式表征的单细胞空间基因组学平台

• 批准号: 10408000
• 负责人: Fei Chen
• 金额: $ 94.64万
• 依托单位: BROAD INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-08 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10408000
• 关键词: ATAC-seq; Adopted; Adoption; Aging; Algorithms; Bar Codes; Biological Assay; Brain; Cells; Chromatin; Chromosomes; Complex; DNA; Data; Data Analyses; Data Collection; Data Set; Development; Disease; Ensure; Epigenetic Process; Gene Expression Profile; Generations; Genes; Genomic approach; Health; Individual; Joints; Libraries; Location; Measurement; Measures; Messenger RNA; Miniaturization; Molecular; Molecular Biology; Organ; Pathologic; Process; Proteins; Protocols documentation; RNA; Regulatory Element; Research Personnel; Resolution; Slide; Structure; Technology; Testing; Tissues; Transposase; Validation; Work; antibody libraries; cell type; computerized tools; data integration; developmental disease; experimental study; genomic platform; genomic tools; high throughput technology; indexing; multimodal data; multimodality; new technology; next generation; novel; oligo (dT); protein expression; single cell sequencing; single-cell RNA sequencing; tissue resource; tool; transcriptome; transcriptome sequencing; transcriptomics

Project Summary/Abstract Cells are the unit building blocks of tissues and organs, thus, to understand how tissues function, it is important to understand where different types of cells reside in health and disease. In this proposal, we propose to develop a novel spatial genomics platform that will enable multi-modal characterization of tissue structure at the single cell level. This will be revolutionary for the study of tissue organization in development and pathological changes in aging and disease. Our tools will enable us to look at RNA, proteins and regulatory elements in DNA all within the tissue context. We will ensure these novel genomic tools are easy to use and adopt, which will be especially powerful for pathological settings, where tissue resources are precious, and adoption of tools is limited by ease of use. The successful completion of this work will lead to a comprehensive toolset for understanding tissue structure from the perspective of single cell organization.

项目总结/摘要 细胞是组织和器官的基本单位，因此，要了解组织是如何 功能，重要的是要了解不同类型的细胞在健康和疾病中的位置。 在这个提议中，我们建议开发一个新的空间基因组学平台， 在单细胞水平上对组织结构进行多模态表征。这将是 组织发育和病理变化研究的革命性进展 衰老和疾病我们的工具将使我们能够观察RNA、蛋白质和调控元件 在组织环境中的DNA中。我们将确保这些新的基因组工具易于使用 并采用，这将是特别强大的病理设置，其中组织资源 是宝贵的，工具的采用受到易用性的限制。圆满完成 这项工作将导致一个全面的工具集，用于了解组织结构， 单细胞组织的观点。