权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The male-determining locus of Culex quinquefasciatus

致倦库蚊的雄性决定基因座

基本信息

批准号：
10176411
负责人：
Zhijian Jake Tu
金额：
$ 19.53万
依托单位：
VIRGINIA POLYTECHNIC INST AND ST UNIV
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10176411
关键词：
Aedes Anopheles gambiae Basic Science Behavior Behavioral Biology Birds Characteristics Chromosome 1 Chromosome Pairing Complex Control Locus Culex (Genus)Culex pipiens Culicidae Data Data Set Development Ectopic Expression Elements Embryonic Development Encephalitis Viruses Evolution Exhibits Filarial Elephantiases Foundations Genes Genetic Genetic Annotation Genetic study Genome Genomics Glean Goals Homologous Gene Human Inherited Investigation Knock-out Link Mammals Messenger RNA Minor Lymphocyte Stimulatory Loci Molecular Molecular Genetics Mosquito Control Mosquito-borne infectious disease Names Pathway interactions Physiological Population Price Proteins RNA Splicing Reporting Research Resources Sex Bias Sex Chromosomes Sex Ratio Sexual Reproduction Taxon Vectorial capacity West Nile virus base climate change disorder control genetic manipulation genomic locus indexing innovation insight interest male member novel novel strategies scaffold sex sex determination transcriptome sequencing transmission process vector

项目摘要

Mosquitoes in the Culex genus are principal vectors of encephalitis viruses and lymphatic filariasis worms. Culex pipiens and C. quinquefasciatus are major vectors of West Nile virus, which is now the leading cause of mosquito-borne disease in the continental US (https://www.cdc.gov/westnile/index.html). There are concerns that climate change may cause further increases in West Nile cases. Members of the C. pipiens species complex, which includes C. quinquefasciatus, C. pipiens, C. molestus and a few other species, show different modes of evolutionary divergence and exhibit behavioral and physiological differences relevant to their vectorial capacity. Therefore, the C. pipiens complex are compelling to study from both biomedical and basic science perspectives. We have a long-standing interest in deciphering the molecular mechanisms by which the male-determining locus (M locus) controls sex-determination and other aspects of male biology in mosquitoes. We focus on C. quinquefasciatus in this proposal for both fundamental and translational considerations. As the male-determining factor (M factor) in C. quinquefasciatus is different from any known M factors in mosquitoes, new insights will be gleaned into sex-determination governed by a novel master regulator in C. quinquefasciatus. The proposed research will also lay the foundation for studies into the evolution of homomorphic sex chromosomes and provide paternally-inherited markers to facilitate investigations into the intriguing evolutionary dynamics of the C. pipiens complex. From an applied perspective, the proposed research will enable innovative applications to control C. quinquefasciatus populations through the introduction of a sex bias towards the non-biting males. By analyzing various RNAseq datasets followed by experimental verifications, we have recently identified two genes in the M locus including a strong candidate for a novel M factor we named cMf. We have also obtained a high quality male C. quinquefasciatus genome assembly by separating paternal and maternal long-read sequences and by Bionano scaffolding. We will pursue two specific aims: 1) systematically characterize the genetic contents of the M and m loci in C. quinquefasciatus; 2) investigate the function of selected M locus genes including cMf. This exploratory R21 will produce a systematic annotation of the genetic contents of the M and m loci and discover the function of the most important M locus genes in C. quinquefasciatus. It will lay the foundation for future research into 1) the mechanism of action of a novel M factor, 2) the evolution of the homomorphic sex chromosomes, 3) the functional elements of the m locus, and 4) applications based on sex ratio manipulation or linkage to the M locus.

库蚊属的蚊子是脑炎病毒和淋巴丝虫的主要媒介。库蚊 pipiens和C.致倦犬是西尼罗河病毒的主要传播媒介，西尼罗河病毒现在是导致蚊子传播的疾病在美国大陆（https：//www.cdc.gov/westnile/index.html）。有人担心气候变化可能导致西尼罗河病例进一步增加。C. pipiens物种复合体，其中包括C. quinquefasciatus、五带弯角藻C. pipiens，C.骚扰者和其他一些物种，表现出不同的模式，进化的分歧，并表现出行为和生理差异相关的向量的能力。因此，C. pipiens复杂的是令人信服的研究从生物医学和基础科学的角度。我们长期以来一直有兴趣破译男性决定基因座的分子机制，（M基因座）控制蚊子的性别决定和雄性生物学的其他方面。我们专注于C。 quinquefasciatus在这一建议的基本和翻译的考虑。作为男性决定者 C中的因子（M因子）。quinquefasciatus不同于蚊子中任何已知的M因子，新的见解将是收集到的性别决定由一个新的主调节C。致倦肌拟议研究还将为研究同型性染色体的进化奠定基础，并提供父系遗传标记，以促进调查的有趣的进化动力学的C。淡色复杂.从应用的角度来看，拟议的研究将使创新的应用，以控制C。通过引入对不咬人的雄性的性别偏见来控制致倦犬种群。通过分析各种RNAseq数据集，然后进行实验验证，我们最近确定了两个在M基因座的基因，包括一个强有力的候选人的一种新的M因子，我们命名为cMf。我们还获得了优质雄性C.通过分离父本和母本长读段进行致倦犬基因组组装序列和Bionano支架。我们将追求两个具体目标：1）系统地描述 M和m基因座的遗传含量。2）研究M位点基因的功能包括CMF。这一探索性的R21将产生一个系统的注释的遗传内容的M和m 基因座，并揭示了C.致倦肌它将奠定基础未来的研究1）一种新的M因子的作用机制，2）同形性别的进化染色体，3）m基因座的功能元件，和4）基于性比操纵的应用，或与M基因座连锁。