Sex ratio distortion in Aedes aegypti
埃及伊蚊性别比例扭曲
基本信息
- 批准号:10681401
- 负责人:
- 金额:$ 56.66万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-09-16 至 2025-08-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAedesAnopheles gambiaeAreaBiologicalCRISPR/Cas technologyCellsChikungunya virusChromatinChromosome 1Chromosome BreakageChromosome PairingChromosomesCulex (Genus)CulicidaeDengueDengue VaccineDengue VirusDengvaxiaDisease OutbreaksEconomicsEquilibriumFemaleFutureGene TargetingGenesGenetic TranscriptionGeographyHaplotypesIncidenceInsecticide ResistanceLaboratoriesLinkMale SterilityMale SterilizationsMeasuresMeiosisMethodsMinor Lymphocyte Stimulatory LociMitosisModelingMolecularMosquito ControlMosquito-borne infectious diseaseMyeloma ProteinsNamesPopulationPopulation GrowthPopulation ReplacementsPreventionProbabilityPropertyRNA-Binding ProteinsRecommendationResearchResistanceSex BiasSex ChromosomesSex DifferencesSex RatioSiteSpecificitySplit GenesSurveysSystemTestingTimeTransgenesTransgenic OrganismsWolbachiaWood materialX ChromosomeY ChromosomeZIKAZika Virusactive controlautosomechikungunyacostdisease transmissiondisorder controlfitnessgenetic approachgenetic manipulationimprovedinnovationinnovative technologiesinsightmalenovelprogramspromoterprototypesexsperm celltooltransgene expressiontransmission processvectorvector controlvector management strategies
项目摘要
Project Summary
Aedes aegypti is a major vector of dengue, chikungunya, and Zika viruses. The global incidence of
dengue has increased dramatically in recent decades. The first dengue vaccine, Dengvaxia®, is
recommended for a very limited population. Current prevention depends mainly on effective vector control,
which is hindered by increasing insecticide-resistance. Novel genetic strategies for vector control are
actively explored, including both population suppression and population replacement. Recent field
releases of either transgenic and Wolbachia-infected sterile male mosquitoes show promising results for
suppressing Aedes populations in areas of modest size. Given the biological, geographical, economic
and cultural complexity facing global mosquito-borne infectious disease control programs, diverse
measures that can be integrated to address various challenges are urgently needed. Female to male sex
ratio distortion is an attractive concept for developing new control measures, as females are responsible
for disease transmission and population growth. Aedes and Culex mosquitoes contain homomorphic sex-
determining chromosomes and maleness is conferred by a dominant male-determining factor (M factor)
within an M locus that is on one of a pair of chromosome 1. Male-biased sex-ratio distortion occurs in
natural populations in Ae. aegypti, which is associated with m-chromosome breakage during meiosis at
a few sites. The Distorter locus is linked to the M-locus on the M-chromosome, conferring a meiotic drive
as it increases the probability of its own inheritance. We recently discovered the M factor, an RNA-binding
protein named Nix and identified the M and m locus in Ae. aegypti. By analysing male and female Illumina
reads across a few strains, we found a number of m-specific repeats that can be targeted for breakage
by CRISPR/cas9 during male meiosis, potentially leading to a male-biased sex ratio distortion. We will
pursue the following specific aims: 1) Systematically characterize components to develop m-shredders.
Research performed here will also improve our understanding of the male germline and the homomorphic
sex chromosomes and facilitate any future genetic manipulations involving the male germline. 2) Develop
m-shredder lines with various degrees of M-linkage to optimize effective population suppression. 3)
Model various applications of m-shredders in population suppression and transgene recall. In summary,
innovative technologies will be used to gain fundamental insights into male germline expression and the
differentiation of the homomorphic sex chromosomes of Ae. aegypti. m-shredders with various levels of
efficiency and transmissibility will be developed and modelled for population suppression and transgene
recall, expanding the tool box for integrated vector management.
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项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Zhijian Jake Tu其他文献
Joint FAO/IAEA Coordinated Research Project on “Exploring genetic, molecular, mechanical and behavioural methods of sex separation in mosquitoes” – an introduction
- DOI:
10.1186/s13071-018-3206-9 - 发表时间:
2018-12-01 - 期刊:
- 影响因子:3.500
- 作者:
Kostas Bourtzis;Zhijian Jake Tu - 通讯作者:
Zhijian Jake Tu
Evaluation of ebony as a potential selectable marker for genetic sexing in Aedes aegypti
- DOI:
10.1186/s13071-025-06709-y - 发表时间:
2025-02-25 - 期刊:
- 影响因子:3.500
- 作者:
Katerina Nikolouli;Austin Compton;Zhijian Jake Tu;Kostas Bourtzis - 通讯作者:
Kostas Bourtzis
Zhijian Jake Tu的其他文献
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{{ truncateString('Zhijian Jake Tu', 18)}}的其他基金
Sex determination and the sex-determining locus in aedine mosquitoes
伊蚊的性别决定和性别决定基因座
- 批准号:
10753608 - 财政年份:2023
- 资助金额:
$ 56.66万 - 项目类别:
The male-determining locus of Culex quinquefasciatus
致倦库蚊的雄性决定基因座
- 批准号:
10176411 - 财政年份:2020
- 资助金额:
$ 56.66万 - 项目类别:
Guy1-conferred female lethality: mechanism and application for mosquito control
Guy1赋予女性致死性:蚊子控制机制及应用
- 批准号:
9221238 - 财政年份:2016
- 资助金额:
$ 56.66万 - 项目类别:
Mechanism and application of male determination in aedine mosquitoes
伊蚊雄性判定机制及应用
- 批准号:
9081697 - 财政年份:2016
- 资助金额:
$ 56.66万 - 项目类别:
Guy1-conferred female lethality: mechanism and application for mosquito control
Guy1赋予女性致死性:蚊子控制机制及应用
- 批准号:
9008131 - 财政年份:2016
- 资助金额:
$ 56.66万 - 项目类别:
Function of a novel male-specific gene in Aedes aegypti
埃及伊蚊中一种新的雄性特异性基因的功能
- 批准号:
8771524 - 财政年份:2014
- 资助金额:
$ 56.66万 - 项目类别:
Function of a novel male-specific gene in Aedes aegypti
埃及伊蚊中一种新的雄性特异性基因的功能
- 批准号:
8856494 - 财政年份:2014
- 资助金额:
$ 56.66万 - 项目类别:
Function of Guy1, a Y-specific gene of Anopheles stephensi
史氏按蚊 Y 特异性基因 Guy1 的功能
- 批准号:
8492308 - 财政年份:2013
- 资助金额:
$ 56.66万 - 项目类别:
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