Monitoring the development of resistance to the novel insecticide clothianidin in Anopheles gambiae

项目摘要/摘要 尽管仍在努力通过新的 使用转基因蚊子来抑制或取代 野生媒介种群，杀虫剂仍然是旨在 在减轻这种疾病的全球负担方面。尤其是在撒哈拉以南非洲， 大规模部署长效杀虫蚊帐和室内残留物 仅仅在两年内，喷洒就大大降低了疟疾的发病率。 几十年。为此，目前抗药性的发展 在媒介种群中观察到的情况被认为是一种非常严重的线索，可以 迅速破坏最近在疟疾控制方面取得的成果。为了保持有效性 用于疟疾病媒控制的杀虫剂，目标干预措施由 充分了解当地人口的易感情况 而不是至关重要的。我们的项目旨在研究抗药性的发展。 新近批准用于室内滞留喷洒的新型杀虫剂--氯硫醚。至 为了实现我们的目标，我们将利用 英国利物浦专科医学院传染病研究中心 喀麦隆的疾病以及概念上和国际上的直接伙伴 参与了喀麦隆防治疟疾的斗争。这一合作将促进 能力建设和技术转让，以促进使用国家-- 最先进的基因组和功能基因组工具，以解决基础 流行国家疟疾病媒的抗药性。具体目标 我们项目的主要内容是：1)监测AN的易感性。冈比亚亚纲对衣藻毒素的影响 喀麦隆，2)评估#年抵抗力的发展和建立 Clothianidin-选定的按蚊种群。冈比亚，以及3)确定遗传 对氯硫菌素产生抗性的基因座。 好了！

Project Summary/Abstract Despite ongoing efforts to diversify malaria control interventions with new technologies such as the use of transgenic mosquitoes to suppress or replace wild vector populations, insecticides remain the cornerstone of strategies aimed at reducing the global burden of this disease. In sub-Saharan Africa in particular, the massive deployment of long-lasting insecticidal nets and indoor residual spraying has contributed to a significant reduction in malaria incidence in just two decades. For this reason, the development of insecticide resistance currently observed among vector populations is regarded as a very serious thread that can quickly undermine recent gains in malaria control. To preserve the effectiveness of insecticides used for malaria vector control, target interventions informed by sufficient knowledge of the susceptibility profile of local populations are more than crucial. Our project aims to study the development of resistance to clothianidin, a new insecticide recently approved for indoor residual spraying. To achieve our goal, we will capitalize on an international collaboration between Liverpool School of Topical Medicine in the UK, Centre for Research in Infectious Diseases in Cameroon as well as notional and international partners directly involved in the fight against malaria in Cameroon. This collaboration will foster capacity building and transfer of technologies in order to promote the use state- of-the-art genomic and functional genomic tools to address the underpinnings of insecticide resistance in malaria vectors in endemic countries. The specific aims of our project are: 1) to monitor the susceptibility of An. gambiae to clothianidin in Cameroon, 2) to evaluate the development and establishment of resistance in clothianidin-selected populations of An. gambiae, and 3) to determine the genetic loci underlying resistance to clothianidin. !