Ultra-sensitive multi-mode laser-scanning imaging system

总结 该提案要求获得资金，以获得一个超灵敏的高定量成像平台， 凝胶，组织学载玻片， 孔板和用于放射性的磷光体成像盒。该应用程序将直接支持12 NIH- 资助研究人员和支持，并大大扩大了由24个NIH赠款支持的研究。中的每 这些研究人员有一些具体的项目，这些项目受益于“赛蒂瓦台风”的一个或多个独特方面 5激光扫描成像仪。在某些情况下，实验涉及超灵敏定量，使蛋白质 从异常低的输入样本中检测。在其他情况下，台风5使增加 提高了通量，扩大了筛选和发现的能力。在其他情况下，实验使 放射性信号的定量，这在Weill Cornell成像核心设施中不再可用。的 Typhoon 5是用来替换一台不能用的荧光成像仪的。台风5号将提供能力， 威尔康奈尔医学院和邻近的机构，并将由一个咨询委员会监督，监督 药理学核心，并因此将积极影响基础和转化NIH资助的研究， 扩大化学生物学、化学生物学和药理学研究。NIH资助的研究 该仪器支持的项目与癌症、免疫学、代谢、药物滥用有关 包括酒精、神经科学、神经变性、骨病和其他途径。

SUMMARY This proposal request funds to acquire an ultrasensitive highly quantitative imaging platform suitable for quantification of fluorescent, chemiluminescent, chromatic, and radioactive signals on gels, histologic slides, well plates, and phosphor imaging cassettes for radioactivity. This application will directly support 12 NIH- funded investigators and support and substantially expand the research supported by 24 NIH grants. Each of these investigators has specific projects that benefits from one or more unique aspects of the Cytiva Typhoon 5 laser-scanning imager. In some cases, the experiments involve ultrasensitive quantification enabling protein detection from exceptionally low input samples. In other instances, the Typhoon 5 enables increased throughput and broadens the capacity for screening and discovery. In other cases, the experiments enable quantification of radioactive signals, which is no longer available at the Weill Cornell Imaging Core facility. The Typhoon 5 is intended to replace a nonfunctional phosphor imager. The Typhoon 5 will provide capabilities to Weill Cornell Medicine and neighboring institutions and will be supervised by an advisory committee, overseen by the Pharmacology Core, and will thus positively impact basic and translational NIH-funded research, expanding chemical biology, chemical biology, and pharmacology research. The NIH-funded research programs supported by this instrumentation has relevance to cancer, immunology, metabolism, drugs of abuse including alcohol, neuroscience, neurodegeneration, bone disease, and other pathways.