Mount Sinai HHEAR Network Untargeted Lab Hub

总体项目总结/摘要 西奈山伊坎医学院将利用我们在环境方面的研究专长， 流行病学，分析化学和临床实践，以更新我们的人类健康暴露分析 资源（HHEAR）实验室网络中心（在赠款周期1中以前称为“CHEARS”）。我们将使用一个 一套“omic”技术，用于测量环境暴露及其在所有生命阶段对 帮助NIH资助的研究人员确定环境如何影响人类健康，发展和风险 疾病在整个生命周期。在过去的3年里，我们扩大了我们的实验室资源，包括液体 处理人员自动化样品制备/等分，并与数据科学家合作自动化数据处理， 加快我们工作的步伐。此外，我们将质谱仪的数量从7台增加到14台， 所有这些都是为了准备这次更新申请。我们将利用我们的实质性机构 投资，包括我们新的3000万美元的暴露组学研究所，以服务于HHEAR实验室网络及其 NIH研究员客户。我们的非目标资源将使用分子环境组学和代谢组学来测量 暴露于环境化学品及其代谢物以及对这些化学品的内部反应 暴露。我们将用金属组学、蛋白质组学和脂质组学来补充这些措施。但我们 认识到健康研究的不断变化，并包括微生物组学和病毒组学， 美国国立卫生研究院资助的研究人员有兴趣将这些措施纳入现有的研究。我们有... 提供给CHEMICAL用户的最先进的分析方法和仪器。虽然我们将 继续为HHEAR客户提供这些行之有效的方法，我们也致力于倾听 HHEAR用户的需求，并开发新的生物标志物，以满足这些需求。我们的发展核心将建立 该公司在创造新方法以测量当前和过去的化学品暴露方面取得了非常成功的工作， 新的生物基质（如牙齿、毛发、胎盘、新生儿干血斑），并开发新的检测方法， 来自HHEAR的目标和环境资源。我们的行政核心将协调规划 以及在所有中心组件之间的内部通信和与HHEAR协调中心的外部通信， 数据中心和其他HHEAR网络集线器。在内部，行政核心将精简和 优先考虑HHEAR工作，评估检测需求，在开发新检测时推广和传播新检测， 协调协议和QA/QC程序，并协调日常运营。我们的中心会建议 申请人对样品要求、样品质量、结果解释、样品收集、储存方案的意见 和样本运输规格，并引导他们最创新的环境健康科学。

PROJECT SUMMARY/ABSTRACT FOR OVERALL The Icahn School of Medicine at Mount Sinai will leverage our research expertise in environmental epidemiology, analytical chemistry and clinical practice to renew our Human Health Exposure Analysis Resource (HHEAR) Laboratory Network Hub (formerly known as “CHEAR” in grant cycle 1). We will use a suite of `omic' technologies to measure environmental exposures and their response across all life stages to help NIH funded researchers determine how the environment affects human health, development and risk of disease across the life span. In the last 3 years we have expanded our laboratory resources to include liquid handlers to automate sample prep/aliquoting and worked with data scientist to automate data processing to speed the pace of our jobs. In addition we doubled the number of mass spectrometers from 7 to 14 and hired additional faculty all in preparation for this renewal application. We will leverage our substantial institutional investments, including our new $30 million Institute for Exposomics, to serve the HHEAR Lab network and its NIH researcher clients. Our Untargeted Resource will use molecular enviromics and metabolomics to measure exposure to environmental chemicals and their metabolites as well as the internal response to those exposures. We will supplement those measures with metallomics, proteomics and lipidomics. However, we are cognizant of the ever-changing landscape of health research and have included Microbiome and Viromics to prempt the NIH funded researchers' interest in including these measures to existing studies. We have state-of- the-art analytical methodologies and instrumentation that were made available to CHEAR users. While we will continue to offer these well-established methods to HHEAR clients, we are also committed to listening to the needs of HHEAR users and develop new biomarkers to meet those needs. Our Developmental core will build upon its highly successful work in creating novel methods to measure current and past chemical exposures in novel biological matrices (e.g. teeth, hair, placenta, neonatal dry blood spots) and develop new assays that arise from HHEAR's targeted and environmental resources. Our Administrative Core will coordinate planning and communication internally among all Hub components and externally with the HHEAR Coordinating Center, Data Center and the other HHEAR Network Hubs. Internally, the Administrative Core will streamline and prioritize HHEAR jobs, assess assay needs, promote and disseminate new assays as they are developed, harmonize protocols and QA/QC procedures and coordinate day-to-day operations. Our Hub will advise applicants on sample requirements, sample quality, results interpretation, sample collection, storage protocols and sample shipping specifications and guide them to the most innovative environmental health science.