Mount Sinai HHEAR Network Untargeted Lab Hub

总体的项目摘要/摘要 位于西奈山的伊坎医学院将利用我们在环境保护方面的研究专长 流行病学、分析化学和临床实践更新我们的人类健康暴露分析 资源(HHEAR)实验室网络中心(在赠款周期1中以前称为“CHEAR”)。我们将使用 一套用于测量环境暴露及其在所有生命阶段对 帮助美国国立卫生研究院资助的研究人员确定环境如何影响人类健康、发育和糖尿病风险 疾病贯穿整个生命周期。在过去的三年里，我们扩大了我们的实验室资源，将液体包括在内 处理人员自动化样品准备/等分，并与数据科学家合作自动化数据处理，以 加快我们工作的步伐。此外，我们将质谱仪的数量增加了一倍，从7台增加到14台，并聘请了 更多的教员都在为这次续签申请做准备。我们将利用我们雄厚的体制力量 投资，包括我们新成立的3000万美元的展览组学研究所，为HHEAR实验室网络及其 美国国立卫生研究院研究员客户。我们的非靶向资源将使用分子环境和代谢组学来测量 暴露于环境化学品及其代谢物以及对这些物质的内部反应 曝光。我们将用金属组学、蛋白质组学和脂类组学来补充这些措施。然而，我们正在 认识到不断变化的健康研究格局，并已将微生物组和病毒学纳入 美国国立卫生研究院资助研究人员将这些措施纳入现有研究的兴趣。我们有状态- 向CHEAR用户提供的最先进的分析方法和仪器。虽然我们会 继续向HHEAR客户提供这些成熟的方法，我们也致力于听取 HHEAR用户的需求，并开发新的生物标志物来满足这些需求。我们的发展核心将建立 在创造新的方法来测量当前和过去的化学暴露方面取得了非常成功的工作 新的生物基质(例如牙齿、头发、胎盘、新生儿干血点)，并开发新的检测方法 产生于HHEAR的目标资源和环境资源。我们的行政核心将协调规划 以及所有枢纽组件之间的内部通信和与HHEAR协调中心的外部通信， 数据中心和其他HHEAR网络枢纽。在内部，行政核心将精简和 优先安排HHEAR工作的优先顺序，评估分析需求，在开发新的分析方法时推广和传播它们， 协调协议和QA/QC程序，协调日常运作。我们的中心将提供建议 申请人关于样品要求、样品质量、结果解释、样品采集、存储协议 和样品运输规范，并引导他们走向最具创新性的环境健康科学。

PROJECT SUMMARY/ABSTRACT FOR OVERALL The Icahn School of Medicine at Mount Sinai will leverage our research expertise in environmental epidemiology, analytical chemistry and clinical practice to renew our Human Health Exposure Analysis Resource (HHEAR) Laboratory Network Hub (formerly known as “CHEAR” in grant cycle 1). We will use a suite of `omic' technologies to measure environmental exposures and their response across all life stages to help NIH funded researchers determine how the environment affects human health, development and risk of disease across the life span. In the last 3 years we have expanded our laboratory resources to include liquid handlers to automate sample prep/aliquoting and worked with data scientist to automate data processing to speed the pace of our jobs. In addition we doubled the number of mass spectrometers from 7 to 14 and hired additional faculty all in preparation for this renewal application. We will leverage our substantial institutional investments, including our new $30 million Institute for Exposomics, to serve the HHEAR Lab network and its NIH researcher clients. Our Untargeted Resource will use molecular enviromics and metabolomics to measure exposure to environmental chemicals and their metabolites as well as the internal response to those exposures. We will supplement those measures with metallomics, proteomics and lipidomics. However, we are cognizant of the ever-changing landscape of health research and have included Microbiome and Viromics to prempt the NIH funded researchers' interest in including these measures to existing studies. We have state-of- the-art analytical methodologies and instrumentation that were made available to CHEAR users. While we will continue to offer these well-established methods to HHEAR clients, we are also committed to listening to the needs of HHEAR users and develop new biomarkers to meet those needs. Our Developmental core will build upon its highly successful work in creating novel methods to measure current and past chemical exposures in novel biological matrices (e.g. teeth, hair, placenta, neonatal dry blood spots) and develop new assays that arise from HHEAR's targeted and environmental resources. Our Administrative Core will coordinate planning and communication internally among all Hub components and externally with the HHEAR Coordinating Center, Data Center and the other HHEAR Network Hubs. Internally, the Administrative Core will streamline and prioritize HHEAR jobs, assess assay needs, promote and disseminate new assays as they are developed, harmonize protocols and QA/QC procedures and coordinate day-to-day operations. Our Hub will advise applicants on sample requirements, sample quality, results interpretation, sample collection, storage protocols and sample shipping specifications and guide them to the most innovative environmental health science.