Targeted Analysis Resource

项目摘要/摘要：有针对性的分析资源 明尼苏达州HHEAR目标分析资源将结合 明尼苏达大学和明尼苏达州公共卫生实验室的研究人员 健康。这一有针对性的分析资源将通过提供最先进的 包括母体在内的直接指示环境化学暴露的生物标志物的定量分析 化合物、代谢物和DNA或蛋白质加合物。此目标分析资源构建在 已在明尼苏达州目标分析中成功应用的既定方法和专业知识 CHEAR计划的资源。有针对性的分析资源将提供及时可靠的量化 与人类健康高度相关的环境和生活方式暴露的三个主要领域的数据： 1.接触烟草特有的化合物； 2.暴露在环境、生活方式和营养毒物和致癌物中； 3.内源化合物和膳食化合物水平的变化。 烟草特有的化合物对于确定接触烟草产品或电子烟至关重要。 它们包括全套尿尼古丁代谢物(尼古丁、可替宁、3‘-羟基可替宁和它们的 血清、干血斑和趾甲中的可替宁和3‘-羟基可替宁； 烟草特有的致癌代谢产物4-(甲基亚硝基)-1-(3-吡啶)-1-丁醇(NNAL)及其 尿液、血液和脚趾甲中的葡萄糖醛酸苷。环境、生活方式和营养毒物以及 致癌物和代谢物包括挥发性有毒物质的硫代谢物，如 丙烯腈(CEMA)、苯(SPMA)、丙烯醛(3-HPMA)、巴豆醛(HMPMA)和环氧乙烷 (HEMA)；呋喃的尿代谢物；多环芳烃(PAH)的尿代谢物，如 1-羟基吡喃(1-HOP)和菲四醇(Phet)；尿金属；全氟烷基和多氟烷基 物质；类阿片、大麻素和特制药物；甲醛的白细胞DNA加合物， 乙醛、丙烯醛和杂环芳胺在福尔马林固定石蜡中的DNA加合物 嵌入的组织。内源性和饮食化合物包括致癌的尿液杂环 芳胺类化合物2-氨基-3，8-二甲基咪唑并[4，5-f]喹恶啉(MeIQx)和2-氨基-1-甲基-6- 苯并[4，5-b]吡啶(PhIP)及其代谢物；头发中的PhIP；组织中的8-羟基脱氧鸟苷； 血浆胡萝卜素、番茄红素、生育酚、抗坏血酸、维生素D衍生物、异前列腺素和尿液 多酚。

PROJECT SUMMARY/ABSTRACT: TARGETED ANALYSIS RESOURCE The Minnesota HHEAR Targeted Analysis Resource will combine the vast analytical chemistry expertise of investigators at the University of Minnesota and the Public Health Laboratory, Minnesota Department of Health. This Targeted Analysis Resource will serve the research community by providing state-of-the art quantitative analyses of biomarkers directly indicative of environmental chemical exposures including parent compounds, metabolites, and DNA or protein adducts. This Targeted Analysis Resource builds on the established methods and expertise that have been applied successfully in the Minnesota Targeted Analysis Resource of the CHEAR program. The Targeted Analysis Resource will provide timely and reliable quantitative data in three major areas of environmental and lifestyle exposures highly relevant to human health: 1. Exposure to tobacco-specific compounds; 2. Exposure to environmental, lifestyle, and nutritional toxicants and cacinogens; 3. Variations in levels of endogenous and dietary compounds. The tobacco-specific compounds are critical for establishing exposure to tobacco products or e-cigarettes. They include the full suite of urinary nicotine metabolites (nicotine, cotinine, 3′-hydroxycotinine and their glucuronides, and nicotine-N-oxide); cotinine and 3′-hydroxycotinine in serum, dried blood spots, and toenails; and the tobacco-specific carcinogen metabolites 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and its glucuronides in urine, blood, and toenails. The environmental, lifestyle, and nutritional toxicants and carcinogens and metabolites include mercapturic acid (MA) metabolites of volatile toxicants such as acrylonitrile (CEMA), benzene (SPMA), acrolein (3-HPMA), crotonaldehyde (HMPMA), and ethylene oxide (HEMA); urinary metabolites of furan; urinary metabolites of polycyclic aromatic hydrocarbons (PAH) such as 1-hydroxypyrene (1-HOP) and phenanthrene tetraol (PheT); urinary metals; per- and polyfluoroalkyl substances; opioids, cannabinoids, and designer drugs; leukocyte DNA adducts of formaldehyde, acetaldehyde, and acrolein; and DNA adducts of heterocyclic aromatic amines in formalin fixed paraffin embedded tissues. The endogenous and dietary compounds include the carcinogenic urinary heterocyclic aromatic amines 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) and 2-amino-1-methyl-6- pheynylimidazo[4,5-b]pyridine (PhIP) and their metabolites; PhIP in hair; tissue 8-hydroxydeoxyguanosine; plasma carotenes, lycopenes, tocopherols, ascorbic acid, vitamin D derivatives, isoprostanes, and urinary polyphenols.