Tobacco Constituent and Biomarker Assessment Core

烟草成分和生物标志物评估核心

• 批准号: 8310412
• 负责人: STEPHEN S HECHT
• 金额: $ 27.51万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8310412
• 关键词: 1,3-Butadiene; 1-Butanol; 2-butenal; Acetaldehyde; Acetylcysteine; Acids; Acrolein; Alkaloids; Benzene; Biological Markers; Carbolines; Carcinogens; Chemicals; Cotinine; DNA; DNA Adducts; Ethylene Oxide; Evaluation; Formaldehyde; Glucuronides; Human; Inflammation; Laboratories; Leukocytes; Malignant Neoplasms; Measures; Methods; Modeling; N' -nitrosonornicotine; Nicotine; Public Health; Research; Research Personnel; Resources; Tobacco; Tobacco smoke; Tobacco use; hydroxycotinine; oxidative damage; prostaglandin M; tool; tumor; urinary

The Tobacco Constituent and Biomarker Assessment Core will provide a unique resource for Projects 1, 2 and 4. Investigators in this Core comprise one of the leading academic laboratories in the world for chemical analysis of tobacco constituents and related metabolites and biomarkers. For Project 1, the Core will quantify several important constituents of tobacco, namely tobacco alkaloids, (3-carbolines, and acetaldehyde and their biomarkers. For Projects 2 and 4, the Core will quantify certain important urinary metabolites of tobacco and tobacco smoke constituents as well as urinary metabolites related to inflammation and oxidative damage. The following urinary metabolites will be quantified: nicotine equivalents (the total of nicotine, cotinine, and trans-3'-hydroxycotinine and their glucuronides), total NNAL (the total of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its glucuronides), total NNN (the total of free and glucuronidated N'-nitrosonornicotine), mercapturic acids of the volatile carcinogens 1,3-butadiene, benzene, acrolein, crotonaldehyde, and ethylene oxide, and the inflammation and oxidative damage biomarkers 11-a-hydroxy-9,15-dioxo-2,3,4,5-tetranorprostane-1,20-dioic acid (PGE-M) and 9,11,15-trihydroxyprosta-5,13-dien-1-oic acid. We will also quantify the formaldehyde-DNA adduct, N6-hydroxymethyldeoxyadenosine in leukocyte DNA, for Project 4. These biomarkers will provide crucial information for the research being carried out in Projects 1, 2 and 4.

烟草成分和生物标记评估核心将为项目1、2和4提供独特的资源。该核心的研究人员包括世界领先的烟草成分和相关代谢物和生物标记化学分析的学术实验室之一。对于项目1，核心项目将量化烟草的几种重要成分，即烟草生物碱、3-碳氢化合物和乙醛及其生物标志物。对于项目2和4，核心将对烟草和烟草烟雾成分的某些重要尿代谢物以及与炎症和氧化损伤有关的尿代谢物进行量化。以下尿代谢物将被量化：尼古丁当量(尼古丁、可替宁和反式-3‘-羟基可替宁及其葡萄糖醛酸化物的总量)、总NNAL(4-(甲基亚硝氨基)-1-(3-吡啶)-1-丁醇及其葡萄糖醛酸化物的总量)、总NNN(游离态和葡萄糖醛酸化的N’-亚硝基或尼古丁的总量)、挥发性致癌物质1，3-丁二烯、苯、丙烯醛、巴豆醛和环氧乙烷的硫代尿酸，以及炎症和氧化损伤生物标志物11-a-羟基-9，15-二氧代-2，3，34，5-四氢前列腺素-1，20-二酸(PGE-M)和9，11，15-三羟基前列腺素-5，13-二烯-1-酸。我们还将对项目4中的甲醛-DNA加合物N6-羟甲基脱氧腺苷进行量化。这些生物标志物将为项目1、2和4中的研究提供关键信息。