BIOMARKERS CORE

生物标志物核心

• 批准号: 8153475
• 负责人: STEPHEN S HECHT
• 金额: $ 18.75万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8153475
• 关键词: 1,3-Butadiene; 1-Butanol; 2-butenal; Abbreviations; Acetylcysteine; Acids; Acrolein; Alkaloids; Anabasine; Arachidonic Acids; Benzene; Biological Markers; Butanones; Carcinogens; Chemicals; Cigarette; Cotinine; DNA; DNA Adducts; Dinoprostone; Ethylene Oxide; Evaluation; Formaldehyde; Glucuronides; Human; Inflammation; Instruction; Laboratories; Leukocytes; Malignant Neoplasms; Methods; Minor; N' -nitrosonornicotine; Nicotine; Parents; Principal Investigator; Procedures; Program Research Project Grants; Public Health; Quality Control; Research; Research Personnel; Resources; Series; Services; Sum; Tobacco; Tobacco use; Tobacco-Associated Carcinogen; Urine; hydroxycotinine; nornicotine; oxidative damage; phenanthrene; prostaglandin M; tobacco carcinogenesis; tool; toxicant; tumor; urinary

The Tobacco Constituent and Biomarker Assessment Core will provide a unique resource for Projects 1-3. Investigators in this Core comprise one of the leading academic laboratories in the world for chemical analysis of tobacco constituents and related metabolites and biomarkers. The following urinary metabolites will be quantified: nicotine equivalents (the total of nicotine, cotinine, and trans-3'-hydroxycotinine and their glucuronides), total NNAL (the total of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its glucuronides), total NNN (the total of free and glucuronidated A/'-nitrosonornicotine), mercapturic acids of the volatile carcinogens 1,3-butadiene, benzene, acrolein, crotonaldehyde, and ethylene oxide, and the inflammation and oxidative damage biomarkers 11-a-hydroxy-9,15-dioxo-2,3,4,5-tetranorprostane-1,20-dioic acid (PGE- M) and 9,11,15-trihydroxyprosta-5,13-dien-1-oic acid. We will also quantify the formaldehyde-DNA adduct, /V^-hydroxymethyideoxyadenosine in leukocyte DNA, and minor tobacco alkaloids in urine. These biomarkers will provide crucial information for the research being carried out in Projects 1-3. RELEVANCE (See instructions): Methods for achieving reduction in public health harm from use of tobacco products are critical for decreasing the human cancer toll of tobacco use. The biomarkers being quantified in this core are representative of many of the major carcinogenic and tumor enhancing constituents known to be present in tobacco products. Quantifying these biomarkers will provide an important tool for the evaluation of tobacco products to determine their potential impact on public health.

烟草成分和生物标志物评估核心将为项目1-3提供独特的资源。 该核心的研究人员组成了世界上领先的化学学术实验室之一， 烟草成分和相关代谢物及生物标志物的分析。以下尿液代谢产物 将定量：尼古丁当量（尼古丁、可替宁和反式-3 '-羟基可替宁及其 总NNAL（4-（甲基亚硝胺基）-1-（3-吡啶基）-1-丁醇及其葡糖苷酸的总和）， 总NNN（游离的和葡萄糖醛酸化的亚硝基去甲烟碱的总量）、挥发物的巯基尿酸 致癌物1，3-丁二烯、苯、丙烯醛、巴豆醛和环氧乙烷，以及炎症 和氧化损伤生物标志物11-α-羟基-9，15-二氧代-2，3，4，5-四去甲前列烷-1，20-二酸（PGE-A）。 M）和9，11，15-三羟基前列腺-5，13-二烯-1-酸。我们还将对甲醛-DNA加合物进行定量， 白细胞DNA中的N-羟甲氧腺苷和尿中的少量烟草生物碱。这些 生物标志物将为项目1-3中正在进行的研究提供关键信息。 相关性（参见说明）： 实现减少烟草制品使用对公共健康危害的方法对于以下方面至关重要： 减少人类因吸烟而患癌症的人数。在这个核心中量化的生物标志物是 代表已知存在于体内的许多主要致癌和肿瘤增强成分 烟草制品.量化这些生物标志物将为烟草的评价提供重要工具 以确定其对公众健康的潜在影响。