BIOMARKERS CORE

生物标志物核心

• 批准号: 8153475
• 负责人: STEPHEN S HECHT
• 金额: $ 18.75万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8153475
• 关键词: 1,3-Butadiene; 1-Butanol; 2-butenal; Abbreviations; Acetylcysteine; Acids; Acrolein; Alkaloids; Anabasine; Arachidonic Acids; Benzene; Biological Markers; Butanones; Carcinogens; Chemicals; Cigarette; Cotinine; DNA; DNA Adducts; Dinoprostone; Ethylene Oxide; Evaluation; Formaldehyde; Glucuronides; Human; Inflammation; Instruction; Laboratories; Leukocytes; Malignant Neoplasms; Methods; Minor; N' -nitrosonornicotine; Nicotine; Parents; Principal Investigator; Procedures; Program Research Project Grants; Public Health; Quality Control; Research; Research Personnel; Resources; Series; Services; Sum; Tobacco; Tobacco use; Tobacco-Associated Carcinogen; Urine; hydroxycotinine; nornicotine; oxidative damage; phenanthrene; prostaglandin M; tobacco carcinogenesis; tool; toxicant; tumor; urinary

The Tobacco Constituent and Biomarker Assessment Core will provide a unique resource for Projects 1-3. Investigators in this Core comprise one of the leading academic laboratories in the world for chemical analysis of tobacco constituents and related metabolites and biomarkers. The following urinary metabolites will be quantified: nicotine equivalents (the total of nicotine, cotinine, and trans-3'-hydroxycotinine and their glucuronides), total NNAL (the total of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its glucuronides), total NNN (the total of free and glucuronidated A/'-nitrosonornicotine), mercapturic acids of the volatile carcinogens 1,3-butadiene, benzene, acrolein, crotonaldehyde, and ethylene oxide, and the inflammation and oxidative damage biomarkers 11-a-hydroxy-9,15-dioxo-2,3,4,5-tetranorprostane-1,20-dioic acid (PGE- M) and 9,11,15-trihydroxyprosta-5,13-dien-1-oic acid. We will also quantify the formaldehyde-DNA adduct, /V^-hydroxymethyideoxyadenosine in leukocyte DNA, and minor tobacco alkaloids in urine. These biomarkers will provide crucial information for the research being carried out in Projects 1-3. RELEVANCE (See instructions): Methods for achieving reduction in public health harm from use of tobacco products are critical for decreasing the human cancer toll of tobacco use. The biomarkers being quantified in this core are representative of many of the major carcinogenic and tumor enhancing constituents known to be present in tobacco products. Quantifying these biomarkers will provide an important tool for the evaluation of tobacco products to determine their potential impact on public health.

烟草组成和生物标志物评估核心将为1-3的项目提供独特的资源。 该核心的研究人员包括世界上领先的学术实验室之一 分析烟草成分以及相关代谢物和生物标志物。以下尿代谢物 将被量化：尼古丁等效物（尼古丁，可替宁和反式3'-羟基霉素及其总计 葡萄糖醛酸苷），总NNAN（总4-（甲基硝基氨基氨基氨基）-1-（3-吡啶基）-1-丁醇及其葡萄糖醛酸苷）， 总NNN（游离和葡萄糖醛酸糖苷的总氮 - 硝基核苷），挥发性的苏联酸 致癌物1,3-丁二烯，苯，丙烯醛，克罗替醛和氧化乙烷以及炎症 和氧化损伤生物标志物11-A-Hydroxy-9,15-Dioxo-2,3,4,5-四甲烷丙烷1,20-二酸（PGE-- m）和9,11,15-三羟基蛋白质-5,13-Dien-1- oic酸。我们还将量化甲醛-DNA加合物， /v^ - 白细胞DNA中的羟基乙酰氧添合核苷，尿液中的少量烟草生物碱。这些 生物标志物将为在项目1-3中进行的研究提供至关重要的信息。 相关性（请参阅说明）： 通过使用烟草产品减少公共卫生危害的方法对 减少烟草使用的人类癌症损失。在此核心中量化的生物标志物是 代表许多已知存在的主要致癌和肿瘤增强成分的代表 烟草产品。量化这些生物标志物将为评估烟草提供重要的工具 确定其潜在影响公共卫生的产品。