BIOMARKERS CORE

生物标志物核心

• 批准号: 8153475
• 负责人: STEPHEN S HECHT
• 金额: $ 18.75万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8153475
• 关键词: 1,3-Butadiene; 1-Butanol; 2-butenal; Abbreviations; Acetylcysteine; Acids; Acrolein; Alkaloids; Anabasine; Arachidonic Acids; Benzene; Biological Markers; Butanones; Carcinogens; Chemicals; Cigarette; Cotinine; DNA; DNA Adducts; Dinoprostone; Ethylene Oxide; Evaluation; Formaldehyde; Glucuronides; Human; Inflammation; Instruction; Laboratories; Leukocytes; Malignant Neoplasms; Methods; Minor; N' -nitrosonornicotine; Nicotine; Parents; Principal Investigator; Procedures; Program Research Project Grants; Public Health; Quality Control; Research; Research Personnel; Resources; Series; Services; Sum; Tobacco; Tobacco use; Tobacco-Associated Carcinogen; Urine; hydroxycotinine; nornicotine; oxidative damage; phenanthrene; prostaglandin M; tobacco carcinogenesis; tool; toxicant; tumor; urinary

The Tobacco Constituent and Biomarker Assessment Core will provide a unique resource for Projects 1-3. Investigators in this Core comprise one of the leading academic laboratories in the world for chemical analysis of tobacco constituents and related metabolites and biomarkers. The following urinary metabolites will be quantified: nicotine equivalents (the total of nicotine, cotinine, and trans-3'-hydroxycotinine and their glucuronides), total NNAL (the total of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its glucuronides), total NNN (the total of free and glucuronidated A/'-nitrosonornicotine), mercapturic acids of the volatile carcinogens 1,3-butadiene, benzene, acrolein, crotonaldehyde, and ethylene oxide, and the inflammation and oxidative damage biomarkers 11-a-hydroxy-9,15-dioxo-2,3,4,5-tetranorprostane-1,20-dioic acid (PGE- M) and 9,11,15-trihydroxyprosta-5,13-dien-1-oic acid. We will also quantify the formaldehyde-DNA adduct, /V^-hydroxymethyideoxyadenosine in leukocyte DNA, and minor tobacco alkaloids in urine. These biomarkers will provide crucial information for the research being carried out in Projects 1-3. RELEVANCE (See instructions): Methods for achieving reduction in public health harm from use of tobacco products are critical for decreasing the human cancer toll of tobacco use. The biomarkers being quantified in this core are representative of many of the major carcinogenic and tumor enhancing constituents known to be present in tobacco products. Quantifying these biomarkers will provide an important tool for the evaluation of tobacco products to determine their potential impact on public health.

烟草成分和生物标志物评估核心将为项目1-3提供独特的资源。