Minnesota CHEAR Exposure Assessment Hub

明尼苏达州 CEAR 暴露评估中心

• 批准号: 9563479
• 负责人: STEPHEN S HECHT
• 金额: $ 98.01万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2019-09-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9563479
• 关键词: Affect; Analytical Chemistry; Area; Biological; Biological Markers; Carcinogens; Chemicals; Child; Child health care; Childhood; Clinical Research; DNA Adducts; DNA analysis; Data; Development; Diet; Disease; Environmental Carcinogens; Epigenetic Process; Exposure to; Goals; Hair; Health; Herbicides; Human; Inflammation; Insecticides; International; Life Style; Link; Mass Spectrum Analysis; Methods; Minnesota; Modification; Monitor; Organophosphates; Pesticides; Procedures; Quality Control; Research Infrastructure; Research Personnel; Resolution; Resource Development; Resources; Sampling; Scientist; Serum; Serum Albumin; Talents; Time; Tobacco; Toxic Environmental Substances; United States; Universities; Urine; Variant; adduct; base; epidemiology study; experience; metabolomics; oxidative damage; prevent; public health relevance; response; toxicant

 DESCRIPTION (provided by applicant): The Minnesota CHEAR Exposure Assessment Hub brings together highly talented investigators from the University of Minnesota and the Minnesota Department of Health with broad and internationally recognized expertise in the identification and analysis of agents that are potentially harmful to human health, including children's health. Our team is unique because our scientists have years of experience in the development and application of state-of-the-art high throughput analytical chemistry methods based mainly on mass spectrometry for the assessment of trace amounts of targeted toxicant or carcinogen metabolites in large clinical and epidemiological studies, with all appropriate sample tracking and quality control methods in place. They have also developed and applied methods for the untargeted analysis of human serum, urine, or fecal samples as well as the analysis of DNA adducts and other biological response parameters in samples from humans. Therefore, we can provide children's health researchers with access to state-of-the-art infrastructure for both targeted and untargeted analysis of biological samples as well as characterization of biological responses associated with those exposures. This goal is firmly embedded within the concept of the exposome and its relationship to children's health. The resources and cores within this Hub are as follows: The Targeted Analysis Resource will use predominantly high throughput mass spectrometric methods with well-established quality control procedures to provide timely and reliable quantitative data relevant to 3 major areas of potential importance with respect to children's health: exposure to general environmental toxicants and carcinogens; exposure to tobacco-specific compounds; and variations in levels of endogenous and dietary compounds. The Untargeted Analysis Resource will conduct high resolution mass spectrometry-based untargeted metabolomics to examine the chemical composition of diverse biological samples relevant to children's health. The Biological Response Indicators Resource will conduct state-of-the-art analyses of carcinogen-DNA adducts, biomarkers of oxidative damage including DNA adducts, biomarkers of inflammation, and parameters of epigenetic modification. The Developmental Core will develop new high throughput methods to quantify organophosphate pesticides, herbicides, and insecticides in urine as well as their adducts to serum albumin, and will establish a multi- residue approach to bio-monitor relevant chemicals in hair. The Administrative Core will coordinate all activities within the Minnesota CHEAR Exposure Assessment Hub and serve as the link between our Hub and the CHEAR Coordinating Center.

 描述（由申请人提供）：明尼苏达州CHEMICAL暴露评估中心汇集了来自明尼苏达大学和明尼苏达州卫生部的才华横溢的研究人员，他们在识别和分析可能对人类健康（包括儿童健康）有害的物质方面具有广泛的国际公认的专业知识。我们的团队是独一无二的，因为我们的科学家在开发和应用最先进的高通量分析化学方法方面拥有多年的经验，这些方法主要基于质谱法，用于在大型临床和流行病学研究中评估痕量的目标毒物或致癌物代谢物，并采用所有适当的样品跟踪和质量控制方法。他们还开发和应用了用于人血清，尿液或粪便样本的非靶向分析以及人类样本中DNA加合物和其他生物反应参数的分析方法。因此，我们可以为儿童健康研究人员提供最先进的基础设施，用于对生物样本进行有针对性和无针对性的分析，以及表征与这些暴露相关的生物反应。这一目标深深植根于“麻烦家庭”概念及其与儿童健康的关系之中。该中心的资源和核心如下：目标分析资源将主要使用高通量质谱方法和完善的质量控制程序，提供与儿童健康潜在重要的三个主要领域相关的及时和可靠的定量数据：暴露于一般环境毒物和致癌物;暴露于烟草特有化合物;以及内源性和膳食化合物水平的变化。非靶向分析资源将进行基于高分辨率质谱的非靶向代谢组学，以检查与儿童健康相关的各种生物样本的化学成分。生物反应指标资源将对致癌物-DNA加合物、氧化损伤生物标志物（包括DNA加合物）、炎症生物标志物和表观遗传修饰参数进行最先进的分析。Developmental Core将开发新的高通量方法来定量尿液中的有机磷农药、除草剂和杀虫剂及其与血清白蛋白的加合物，并将建立一种多残留方法来生物监测头发中的相关化学物质。行政核心将协调明尼苏达州CHEMICAL暴露评估中心内的所有活动，并作为我们的中心和CHEMICAL协调中心之间的联系。