Minnesota CHEAR Exposure Assessment Hub

 描述(由申请人提供)：明尼苏达州CHEAR暴露评估中心汇集了来自明尼苏达大学和明尼苏达州卫生部的才华横溢的调查人员，他们在识别和分析潜在危害人类健康(包括儿童健康)的制剂方面拥有广泛和国际公认的专业知识。我们的团队是独一无二的，因为我们的科学家在开发和应用最先进的高通量分析化学方法方面拥有多年的经验，这些方法主要基于质谱学，用于评估大型临床和流行病学研究中的痕量目标毒物或致癌代谢物，并建立了所有适当的样品跟踪和质量控制方法。他们还开发和应用了对人类血清、尿液或粪便样本进行非靶向分析的方法，以及分析人类样本中DNA加合物和其他生物反应参数的方法。因此，我们可以为儿童健康研究人员提供最先进的基础设施，用于有针对性和无针对性地分析生物样本，以及与这些暴露相关的生物反应的特征。这一目标牢牢植根于曝光组的概念及其与儿童健康的关系。该中心的资源和核心如下：目标分析资源将主要使用高通量质谱学方法和完善的质量控制程序，以提供与儿童健康潜在重要的3个主要领域相关的及时和可靠的定量数据：暴露于一般环境毒物和致癌物；暴露于烟草特有化合物；以及内源化合物和膳食化合物水平的变化。非靶向分析资源将进行基于高分辨率质谱学的非靶向代谢组学，以检查与儿童健康相关的各种生物样本的化学成分。生物反应指标资源将对致癌物-DNA加合物、氧化损伤的生物标记物(包括DNA加合物)、炎症的生物标记物和表观遗传修饰参数进行最先进的分析。开发核心将开发新的高通量方法来量化尿液中的有机磷杀虫剂、除草剂和杀虫剂及其与血清白蛋白的加合物，并将建立一种多残留方法来生物监测头发中的相关化学物质。行政核心将协调明尼苏达州CHEAR暴露评估中心内的所有活动，并作为我们的中心和CHEAR协调中心之间的纽带。

 DESCRIPTION (provided by applicant): The Minnesota CHEAR Exposure Assessment Hub brings together highly talented investigators from the University of Minnesota and the Minnesota Department of Health with broad and internationally recognized expertise in the identification and analysis of agents that are potentially harmful to human health, including children's health. Our team is unique because our scientists have years of experience in the development and application of state-of-the-art high throughput analytical chemistry methods based mainly on mass spectrometry for the assessment of trace amounts of targeted toxicant or carcinogen metabolites in large clinical and epidemiological studies, with all appropriate sample tracking and quality control methods in place. They have also developed and applied methods for the untargeted analysis of human serum, urine, or fecal samples as well as the analysis of DNA adducts and other biological response parameters in samples from humans. Therefore, we can provide children's health researchers with access to state-of-the-art infrastructure for both targeted and untargeted analysis of biological samples as well as characterization of biological responses associated with those exposures. This goal is firmly embedded within the concept of the exposome and its relationship to children's health. The resources and cores within this Hub are as follows: The Targeted Analysis Resource will use predominantly high throughput mass spectrometric methods with well-established quality control procedures to provide timely and reliable quantitative data relevant to 3 major areas of potential importance with respect to children's health: exposure to general environmental toxicants and carcinogens; exposure to tobacco-specific compounds; and variations in levels of endogenous and dietary compounds. The Untargeted Analysis Resource will conduct high resolution mass spectrometry-based untargeted metabolomics to examine the chemical composition of diverse biological samples relevant to children's health. The Biological Response Indicators Resource will conduct state-of-the-art analyses of carcinogen-DNA adducts, biomarkers of oxidative damage including DNA adducts, biomarkers of inflammation, and parameters of epigenetic modification. The Developmental Core will develop new high throughput methods to quantify organophosphate pesticides, herbicides, and insecticides in urine as well as their adducts to serum albumin, and will establish a multi- residue approach to bio-monitor relevant chemicals in hair. The Administrative Core will coordinate all activities within the Minnesota CHEAR Exposure Assessment Hub and serve as the link between our Hub and the CHEAR Coordinating Center.