Understanding Down Syndrome Brain Development Using Human iPSC-Based Mouse Chimeras

使用基于人类 iPSC 的小鼠嵌合体了解唐氏综合症大脑发育

• 批准号: 10179682
• 负责人: Peng Jiang
• 金额: $ 41.76万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-03-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10179682
• 关键词: Affect; Animal Behavior; Animals; Behavior; Behavioral; Biological; Brain; CRISPR/Cas technology; Candidate Disease Gene; Cells; Chimera organism; Chromosome 21; Cognitive deficits; Development; Disease; Down Syndrome; Environment; Exhibits; Gene Expression; Gene Expression Profile; Generations; Genes; Genetic; Genetic Transcription; Hippocampus (Brain); Homeostasis; Human; Human Chromosomes; IFNAR1 gene; Immune; Impairment; In Vitro; Individual; Intellectual functioning disability; Interferons; Learning; Link; Maintenance; Memory; Microglia; Mind; Modeling; Molecular; Mus; Neurons; Organoids; Orthologous Gene; Pathogenesis; Pathogenicity; Pathologic; Pathology; Patients; Performance; Phenotype; Plant Roots; Play; Property; Regulation; Research; Rodent; Role; Specific qualifier value; Synapses; Synaptic plasticity; System; Technology; Testing; Tissues; Transgenic Mice; Trisomy; base; brain abnormalities; brain cell; brain tissue; cell type; developmental disease; disease phenotype; dosage; improved; in vitro Model; in vivo; induced pluripotent stem cell; induced pluripotent stem cell technology; insight; macrophage; memory process; mouse model; nerve stem cell; neural circuit; neural network; new therapeutic target; novel; novel therapeutics; response; single-cell RNA sequencing; stem cells; synaptic function; synaptic pruning; tool; transcriptome

Down syndrome (DS), caused by triplication of human chromosome 21 (HSA21), is the most common genetic origin of intellectual disability. Studying DS disease mechanism is challenging because functional human DS brain tissues are scarcely available and transgenic mouse models of DS demonstrate incomplete/inaccurate expression of HSA21 genes. The advent of human induced pluripotent stem cell (hiPSC) technology has led to the generation of DS patient-derived hiPSCs, which presents an unprecedented opportunity for studying the pathogenesis of DS with unlimited human brain cells in vitro. While using the hiPSC-based in vitro models, basic aspects of the disease phenotypes can be examined, the disruption of neural circuits in the developing brain under disease conditions remains to be studied with hiPSCs. Ultimately, specific developmental and disease mechanisms can only be modeled in live animals to identify links between cellular phenotypes and behavioral performance. Therefore, we propose to employ hiPSC-based chimeric mouse brain models to study the neuropathophysiology of DS in vivo. Microglia play critical roles in brain development and are also an active player in learning and memory processes. Surprisingly, very little information is available on how trisomy of HSA21 alters the development and functions of microglia and what roles microglia play in the abnormal brain development and cognitive deficits in DS. In addition, mounting evidence indicates that rodent microglia are not able to fully mirror the properties of human microglia in normal and disease conditions. In this study, we will use our recently created hiPSC microglial chimeric mouse model to unravel the role of microglia in DS pathogenesis in an in vivo system with intact neural networks. We hypothesize that unlike engrafted normal human microglia, engrafted diseased DS human microglia will show abnormal biological properties and functions, such as synaptic pruning function in vivo. These abnormal properties of DS microglia will result in their negative regulation of the synaptic activity and plasticity of the hippocampal neural network, critically contributing to the cognitive deficits seen in DS. This hypothesis will be tested in three specific aims. Aim 1: we will determine the differences between DS and control hiPSC-derived microglia in vivo in human microglial chimeric mouse brains. Aim 2: Using the microglial chimeric mouse model, we will further examine the impact of integration of DS microglia on synaptic plasticity of the hippocampus and learning and memory behavior of the animals. Aim 3: We will normalize the expression of the HSA21 genes by CRISPR/Cas9 to examine how this will alter the properties of DS microglia. Moreover, single-cell RNA-sequencing analysis of hiPSC microglial chimeric mouse brains will be performed to compare gene expression profiles of control and DS microglia. Findings from our study using a powerful, new hiPSC microglial chimeric mouse model will provide novel insights into the pathological roles of human microglia in DS. Identifying the potential molecules that can be targeted to improve microglial function may provide a new therapeutic avenue for the treatment of DS.

唐氏综合征(DS)是由人类21号染色体三倍体(HSA21)引起的最常见的遗传病 智力残疾的起源。对DS疾病机制的研究具有挑战性，因为功能性人类DS 脑组织几乎不可用，DS转基因小鼠模型显示不完整/不准确 HSA21基因的表达。人类诱导多能干细胞(HiPSC)技术的出现使 DS患者来源的HiPSCs的产生，为研究 体外培养无限人脑细胞的DS发病机制。在使用基于HiPSC的体外模型时，Basic 可以检查疾病表型的各个方面，即发育中的大脑神经回路的中断 在疾病条件下，仍需用HiPSCs进行研究。归根结底，特定的发育和疾病 机制只能在活体动物中建模，以确定细胞表型和行为之间的联系 性能。因此，我们建议使用基于HiPSC的嵌合小鼠脑模型来研究 丹参体内神经病理生理学研究。小胶质细胞在脑发育中起着关键作用，也是一种活跃的 在学习和记忆过程中发挥作用。令人惊讶的是，几乎没有关于三体的信息 HSA21改变小胶质细胞的发育和功能以及小胶质细胞在异常脑中的作用 DS患者的发育和认知缺陷。此外，越来越多的证据表明，啮齿动物的小胶质细胞并不是 能够完全反映正常和疾病条件下人类小胶质细胞的特性。在这项研究中，我们将使用 我们最近建立的HiPSC小胶质细胞嵌合小鼠模型揭示了小胶质细胞在DS发病机制中的作用 在拥有完整神经网络的活体系统中。我们假设，与植入正常人类小胶质细胞不同， 植入患病的DS人小胶质细胞将显示出异常的生物学特性和功能，如突触 体内的修剪功能。DS小胶质细胞的这些异常特性将导致它们对 海马神经网络的突触活性和可塑性，对认知障碍起关键作用 在DS中看到。这一假设将在三个具体目标上得到检验。目标1：我们将确定 DS和对照人小胶质细胞嵌合小鼠脑内hPSC来源的小胶质细胞。目标2：使用 小胶质细胞嵌合小鼠模型，我们将进一步检测整合DS小胶质细胞对突触的影响 海马区的可塑性和动物的学习记忆行为。目标3：我们将实现 在CRISPR/Cas9中表达HSA21基因，以检测这将如何改变DS小胶质细胞的特性。 此外，将对HiPSC嵌合小鼠脑进行单细胞RNA测序分析，以 比较对照组和DS小胶质细胞的基因表达谱。我们的研究发现使用了一种强大的、新的 HiPSC小胶质细胞嵌合小鼠模型将为人类小胶质细胞的病理作用提供新的见解 在DS中。识别可以靶向改善小胶质细胞功能的潜在分子可能提供一种新的 治疗DS的治疗途径。