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Animal, Behavior and Tissue Core

动物、行为和组织核心

基本信息

批准号：
10496282
负责人：
EBENEZER N YAMOAH
金额：
$ 48.63万
依托单位：
UNIVERSITY OF NEVADA RENO
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2028-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10496282
关键词：
Action Potentials Address Affect Age Distribution Aging Animal Model Animals Auditory Auditory area Auditory system Backcrossings Behavior Binaural Biological Markers Brain Stem Breeding Clinical Cochlear nucleus Code Complex Computer Analysis Cryopreservation Demyelinations Detection Development Disasters Disease Disease Outbreaks Exhibits FDA approved Functional disorder Gene Targeting Generations Genes Genetic Genetically Engineered Mouse Genetically Modified Animals Genotype Goals Human Impaired cognition Individual Inhibitory Synapse Joints Knock-in Mouse Laboratories Left Link Maps Mental Depression Modeling Molecular Mus Nerve Degeneration Neural Pathways Neurons Outcome Persons Pharmaceutical Preparations Phase Pitch Perception Presbycusis Program Research Project Grants Property Proteomics Public Health Quality of life Research Resources Rodent Role Sensorineural Hearing Loss Services Social isolation Speech Intelligibility Speed Statistical Data Interpretation Stimulus Stretching Synapses Synaptic plasticity Testing Therapeutic Tissues Transgenic Mice United States National Institutes of Health age related aged aging auditory system aging population animal facility auditory pathway calbindin calretinin combat cost effective design diphtheria toxin receptor excitotoxicity experimental study genetic approach handbook inducible Cre innovation interest mouse model neural neural hearing loss neural network neuron loss neurosensory novel optogenetics otoacoustic emission programs progressive hearing loss response senescence sound tool

项目摘要

Abstract Development and utilization of aged and genetically engineered mouse models are crucial to understanding the roles of specific genes in age-related hearing loss (ARHL). We will require strain-specific aged (senescent) mice that are not available commercially or are produced at the NIA-supported facility at the NIH, http://www.nia.nih.gov/research/dab/aged-rodent-colonies-handbook. All projects, Core C and Core D within the proposed PPG makes extensive use of these aged mice, and utilize a variety of genetic approaches to study the structural and functional remodeling in the auditory neural pathways. The proposed PPG will require the use of gene-targeted mouse models to study the mechanistic underpinning in the aging auditory system. The purpose of the mouse core is to provide services for the establishment of a strain-specific aged mouse colony and the generation and management of gene-targeted and transgenic mice. Founder mice will be identified, bred, and maintained in an animal facility at UNR. Additionally, several mouse lines will be bred and aged and supplied for experiments proposed in all three projects, Core C and Core D. The Core will also be responsible for maintaining and distributing mouse lines to each individual project. This will provide the most cost effective sharing and utilization of the mouse models. In addition, the Core will also cryopreserve critical lines in case of an inadvertent loss. Finally, the Core will be a resource for other laboratories interested in mouse models of ARHL.

摘要开发和利用老年和基因工程小鼠模型对于理解特定基因在年龄相关性听力损失（ARHL）中的作用。我们将需要特定菌株的老化（衰老）没有市售的小鼠或在NIH的NIA支持的设施中生产的小鼠， http://www.nia.nih.gov/research/dab/aged-rodent-colonies-handbook.所有项目，核心C和核心D 所提出的PPG广泛使用这些老年小鼠，并利用各种遗传方法，研究听觉神经通路的结构和功能重构。拟议的PPG将需要使用基因靶向小鼠模型研究衰老听觉系统的机制基础。小鼠核心的目的是为建立品系特异性老龄小鼠提供服务克隆以及基因靶向和转基因小鼠的产生和管理。创始人老鼠将是在UNR的动物设施中鉴定、繁殖和饲养。此外，将培育几种小鼠品系，老化并提供给所有三个项目（核心C和核心D）中提议的实验。核心也将是负责维护和分配鼠标线到每个单独的项目。这将提供最成本有效地共享和利用小鼠模型。此外，核心还将冷冻保存关键的线，以防意外损失。最后，该核心将成为其他感兴趣的实验室的资源， ARHL小鼠模型。