Animal, Behavior and Tissue Core

动物、行为和组织核心

• 批准号: 10496282
• 负责人: EBENEZER N YAMOAH
• 金额: $ 48.63万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10496282
• 关键词: Action Potentials; Address; Affect; Age Distribution; Aging; Animal Model; Animals; Auditory; Auditory area; Auditory system; Backcrossings; Behavior; Binaural; Biological Markers; Brain Stem; Breeding; Clinical; Cochlear nucleus; Code; Complex; Computer Analysis; Cryopreservation; Demyelinations; Detection; Development; Disasters; Disease; Disease Outbreaks; Exhibits; FDA approved; Functional disorder; Gene Targeting; Generations; Genes; Genetic; Genetically Engineered Mouse; Genetically Modified Animals; Genotype; Goals; Human; Impaired cognition; Individual; Inhibitory Synapse; Joints; Knock-in Mouse; Laboratories; Left; Link; Maps; Mental Depression; Modeling; Molecular; Mus; Nerve Degeneration; Neural Pathways; Neurons; Outcome; Persons; Pharmaceutical Preparations; Phase; Pitch Perception; Presbycusis; Program Research Project Grants; Property; Proteomics; Public Health; Quality of life; Research; Resources; Rodent; Role; Sensorineural Hearing Loss; Services; Social isolation; Speech Intelligibility; Speed; Statistical Data Interpretation; Stimulus; Stretching; Synapses; Synaptic plasticity; Testing; Therapeutic; Tissues; Transgenic Mice; United States National Institutes of Health; age related; aged; aging auditory system; aging population; animal facility; auditory pathway; calbindin; calretinin; combat; cost effective; design; diphtheria toxin receptor; excitotoxicity; experimental study; genetic approach; handbook; inducible Cre; innovation; interest; mouse model; neural; neural hearing loss; neural network; neuron loss; neurosensory; novel; optogenetics; otoacoustic emission; programs; progressive hearing loss; response; senescence; sound; tool

Abstract Development and utilization of aged and genetically engineered mouse models are crucial to understanding the roles of specific genes in age-related hearing loss (ARHL). We will require strain-specific aged (senescent) mice that are not available commercially or are produced at the NIA-supported facility at the NIH, http://www.nia.nih.gov/research/dab/aged-rodent-colonies-handbook. All projects, Core C and Core D within the proposed PPG makes extensive use of these aged mice, and utilize a variety of genetic approaches to study the structural and functional remodeling in the auditory neural pathways. The proposed PPG will require the use of gene-targeted mouse models to study the mechanistic underpinning in the aging auditory system. The purpose of the mouse core is to provide services for the establishment of a strain-specific aged mouse colony and the generation and management of gene-targeted and transgenic mice. Founder mice will be identified, bred, and maintained in an animal facility at UNR. Additionally, several mouse lines will be bred and aged and supplied for experiments proposed in all three projects, Core C and Core D. The Core will also be responsible for maintaining and distributing mouse lines to each individual project. This will provide the most cost effective sharing and utilization of the mouse models. In addition, the Core will also cryopreserve critical lines in case of an inadvertent loss. Finally, the Core will be a resource for other laboratories interested in mouse models of ARHL.

摘要 衰老和基因工程小鼠模型的开发和利用对理解 特定基因在年龄相关性耳聋(ARHL)中的作用我们将要求特定品系的老化(衰老) 不能在商业上买到或在NIA支持的NIH设施中生产的老鼠， Http://www.nia.nih.gov/research/dab/aged-rodent-colonies-handbook.所有项目，核心C和核心D 建议的PPG广泛利用这些衰老的小鼠，并利用各种遗传方法来 研究听神经通路的结构和功能重塑。拟议的PPG将需要 使用基因靶向的小鼠模型来研究衰老听觉系统的机制。 小鼠核心的目的是为建立品系特异性衰老小鼠提供服务 克隆以及基因靶向和转基因小鼠的产生和管理。方正老鼠将会成为 在UNR的动物设施中进行鉴定、繁殖和维护。此外，还将培育几个老鼠品系，并 为所有三个项目中提议的实验提供老化和供应，核心C和核心D。核心也将是 负责维护和分发鼠标线到每个单独的项目。这将提供最大的 具有成本效益的鼠标模型共享和利用。此外，堆芯还将进行关键的冷冻保存 线路，以防意外丢失。最后，核心将成为感兴趣的其他实验室的资源 急性淋巴细胞性白血病小鼠模型。