Bioanalytical and Statistics Core

摘要：核心B 生物分析和统计核心B是一个由8名调查人员组成的中央资源， 在了解危险影响方面的互补专业知识和丰富的协作经验 环境物质对人体健康的影响。与核心A(分析)一起，核心B的目标是 检测人体和环境中接触危险化学品及其影响的生物标志物。这个 CORE的宗旨是为加州大学戴维斯分校提供综合、高质量的生物分析和统计服务- NIEHS超级基金研究计划。 生物分析组件提供了一套广泛的分析方法，从过去25年的SRP发展而来 支持，以评估危险化学品的存在和/或这些材料对选定的 生物途径。核心B继承了项目3(免疫分析)的成熟生物分析方法，以及 以前的项目5(基于细胞的分析)。目前有超过25种免疫检测方法可用于检测各种有害物质 化学制品。基于细胞的分析评估与各种受体的化学相互作用作为效应的生物标记物。 这些检测中的大多数已经是EPA Tox21筛查计划的一部分。特异性的酶分析 路径也由核心提供。这些生物分析方法是项目1的组成部分 (生物修复)，因为他们的目标是识别与核心C有关的人类或生态系统健康问题的有毒样本 (社区参与)。项目1和核心A(分析)将确定样本测试中的有毒成分 生物分析方法呈阳性，那么免疫分析将在这个核心转移到社区来建立 他们有能力评估已识别的毒物的发生情况，以帮助他们确定其接触情况。 项目3(免疫分析)、项目4(MOS与心脏毒性)和项目5(暴露诱导的内质网应激)如下 为核心B开发方法，并因此受益于核心人员提供的经验，这将导致 用于暴露评估的有效、可靠的工具。 核心B包括四名博士级别的统计学家，他们在协作方面拥有丰富的相关经验 多学科研究。这些调查人员与该中心的项目和核心合作，以完善他们的 建议，并确定需要专门统计专业知识的研究和教育活动。至 促进有效、经济和持续的支持，主要和次要统计顾问 已分配到每个中心项目或核心。指定任务是为了促进有效的协调 具备专门的统计技能，满足个别项目和核心的特殊需要。 核心的质量保证和管理计划使其能够识别和应对新出现的 中心面临的机遇或挑战，最大限度地提高UCD的可靠性、实用性和影响力 SRC。

ABSTRACT: CORE B The Bioanalytical and Statistics Core B is a centralized resource comprised of eight investigators with complementary expertise and substantial collaborative experience in understanding the impact of hazardous environmental substances on human health. Along with Core A (Analytical), Core B shares the goal of detecting biomarkers of exposure to and effect of hazardous chemicals in humans and the environment. The Core's purpose is to provide integrated, high-quality bioanalytical and statistical services to the UC Davis – NIEHS Superfund Research Program. The bioanalysis component provides a broad suite of assays, developed from the past 25 years of SRP support, to assess the presence of hazardous chemicals and/or effects of these materials on selected biological pathways. Core B inherits mature bioanalytical methods from Project 3 (Immunoassays) and the previous Project 5 (Cell-Based Assays). More than 25 immunoassays are available for various hazardous chemicals. Cell-based assays assess chemical interaction with a variety of receptors as biomarkers of effect. Most of these assays are already part of the EPA Tox21 screening program. Enzyme assays for specific pathways are also be provided by the Core. These bioanalytical methods are integral to Project 1 (Bioremediation) as they aim to identify toxic samples of human or ecosystem health concern to Core C (Community Engagement). Project 1 and Core A (Analytical) will identify toxic components of samples testing positive in bioanalysis methods, then immunoassays in this Core will be transferred to the community to build their capacity to assess the occurrence of the identified toxicant to help them determine their exposures. Projects 3 (Immunoassays), 4 (MOS and Cardiac Toxicity) and 5 (Exposure-induced ER Stress) are developing methods for Core B and as such benefit from experience provided by Core personnel that will result in valid, robust tools for exposure assessment. Core B includes four PhD-level statisticians with substantial relevant experience in collaborative multidisciplinary research. These investigators have worked with the Center's Projects and Cores to refine their proposals and to identify the research and educational activities requiring specialized statistical expertise. To facilitate effective, economical and continuous support, primary and secondary statistical consultants have been assigned to each Center Project or Core. Assignments were specified to promote an effective alignment of specialized statistical skills with the particular needs of individual Projects and Cores. The Core's quality assurance and management plans allow it to identify and respond to emerging opportunities or challenges confronting the Center, maximizing the reliability, utility and impact of the UCD SRC.